The Changes And Pathophysilogical Significance Of Neuropeptides (CGRP,SP) In Skin After Burn

Calcitonin gene-related peptide (CGRP) and substance P (SP) are two putative neuropeptides widely distributed in the central and peripheral nervous systems. CGRP and SP have been demonstrated co-existing in sensory neuron, axons surrounding dermal blood vessels, and unmyelinated nerve endings in skin. A series of evidence indicated that components of the cutaneous nervous system interact with multiple types of cells in the skin to mediate the important actions in local inflammation and wound healing. The changes of CGRP and SP during early period after burn and its influence on edema and wound healing were studied in the present study in order to reveal the mechanism of pathophisiological changes by burn.Study content:1. The distributions of CGRP- and SP-IR positive nerves in skin, adrenal gland and jejunum were observed and measured by immunohistochemical method in rats before and after burn.2. The expressions of CGRP and SP mRNA were observed by in situ hybridization in both normal and burn areas of rat skin.3. The micro-vessel permeability was determined in several internal organs of rat after burn as well as in local skin tissue after injected with CGRP and SP.4. The effects of CGRP and SP on proliferation of keratinocyte, fibroblast and endothelial cell were measured by cell culture in vitro.Main results:1. The SP- and CGRP-IR positive nerves distributed densely in whole skin and more richly surrounding hair follicles, epidermis and blood vessels. Both the densities...