The Studies Of Etiopathogenisis And Pathogenesis About Phlegm Obstruct The Uterus (3)

Objective: To explore the constitution of phlegm-damp type infertility patients and the action mechanism of Cang-Fu-Dao-Tan Tang. To detecting the db/db mice glycolipid metabolism and generate function and to searching for the signal conduction of leptin in mouse's endometrium.Method: Adult, female C57BL/6J mice (Jackson Laboratory, Bar Harbor, ME) were used in these studies. Litter mate wide types (B6.Cg-m +/+ Lepr^db), as well as diabetic (db/db) mutant genotypes, were matched for the experiment. All mice were housed one per cage, under controlled environmental conditions (23C). After two weeks, the vaginal smear was done at 8'oclock every morning. Then, all the mice were executed, the uterus were collected for the detection of the expression of JNK2, STAT3, P-STAT3, SOCS3 and OB-Rb. The serum was reserved for the assay of the glucose, insulin, LDL-c, E2, P, FSH and LH. Before that, all mice were injected leptin (two hours before). The weight of each mouse, their ovary and their fat pads were also recorded. We compared the BMI E₂ corporin leptin thickness of endometrium between patient and healthy ones first. Then compared the BMI E₂ corporin leptin thickness of endometrium of before and behind treatment.Results: All the db/db mice showed the same profile of the glucose and fat metabolism which were hyperglycemia, hyperinsulinemia and hyperlipidemia, significantly higher than the groups of wide types. Moreover, they were acyclic (i.e., persistent diestrus) and anovulatory (i.e., lack of ovarian luteinization), uterus depauperated and diminished steroidogenic capacity. It's clinical feature consistent with the phlegm-damp type infertility. The important reason of infertile was that the obstacle of signal conduction of lepin in endometria influence embryo nidation.The expression of JAK2 in endometrium was in conformity with OB-Rb, and OB-Rb was the main proteinum to activating JAK2.The SOCS3 and PIAS3 were not only participating in signal conduction of leptin. The pathogenesis of the phlegm-damp type infertility was phlegm obstruct the uterus, and the modern biology foundation of phlegm obstruct the uterus were the obstacle of signal conduction of lepin in endometria.The phlegm-damp type infertility patients had the characteristic of obesity,high level leptin, thin endometrium. The Cang-Fu-Dao-Tan Tang could reduce weight, could adjust the level of leptin and the thickness of endometrium.Conclusion: The level of leptin influenced the thickness of endometrium.This study demonstrated that the Cang-Fu-Dao-Tan Tang can improve conception by reducing BMI, adjusting the level of leptin and the thickness of endometrium.