Experimental Study In Effects Of Xia Xi Oral Liquid On Neuron-endocrine-immune Network In Spontaneously Hypertensive Rats

In the present, hypertension has become one of the most common disease in China andhas often led to serious cardio-cerebral vascular complication. It has obtained great advancesin essential hypertension's treatment by Chinese medicine. While the function mechanism ofChinese medicine need to be further investigated. Xia xi Oral Liquid is a proved recipe forhypertension, which is worked out by the author's tutor Prof Huang Yong Sheng, deducingfrom the theory that the main mechanism of EH is the maladjustment of liver and kidney anddamage of superficial venues and lymph vessels. Clinical application has indicated that thisrecipe is quite effective in reducing blood pressure and improve clinical symptom. The furtherstudy indicates that the recipe could improve blood flowing deformation and ameliorateendothelium function of vascular in Spontaneously hypertensive rats (SHR).Based on furtherstudy, this article been performed from the aspects of reference review, theory study as well asexperiment on SHR to observe its effects on neuron-endocrine-immune network.Reference reviewThe essay reviewed from the advances of such fields as followed: the ill-name, aetiologyand pathogenesis for EH, differential syndrome diagnosis, the substantial bases of thedifferential syndrome diagnosis, the treatment of the Chinese medicine, which is in ancientand modern times,moreover, included the evaluations and prospects on the criterions ofdiagnosis and curative effect as well as the problems lying in the selection of treatment basedon the differential syndrome diagnosis or scientific study. This essay summarized theexperimental study results and advances on the principle of neuron-endocrine-immunenetwdrk in EH according to such aspects as nervous intermediary, cell molecules, endocrinesystem and mutual regulation during the pathology process in EH. It also included evaluationsand prospects on these above.Theory studyBy inducing and sorting out the reference, we put forward the theory that the main bases of EH is the maladjustment of liver and kidney and the crux is the damage of superficialvenues and lymph vessels by poison, we expound and prove the inner link of adjustment ofliver and kidney and removing toxicity and obstruction, then put forward the theory that andremoving the toxicity and the obstruction is a valid path of treat Eli. Xia Xi Oral Liquid isoriginated from this theory which precipitating the point of adjustment of liver and kidney andthe characteristics of removing the toxicity and the obstruction, which provides theexperimental and theoretical bases for both its clinical application and its drug development.Experimental study40 12-week-old male SHR were divided randomly into SHR placebo group, Xia Xi OralLiquid control group, captopril control group and Irbesartanl control group, and 10 weightmatched male normotensive WKY rats were set as normal control(WKY group).Observe suchaspects as followed after administration 8 weeks.1 The influence of Xia Xi Oral Liquid to the function and structure to cardiovascularsystem1.1 behavior and general condition effect: Xia Xi Oral Liquid can improve the behavior andgeneral condition in SHR. That indicates that Xia Xi Oral Liquid has regulation function tocenter nervous system.1.2 Decreasing blood effect: Xia Xi Oral Liquid can restrain the tendency that the blood willrise along with aging in SHR. It has a significant statistic sense between the Xia Xi OralLiquid treated group and the SHR placebo group after administration 4 weeks(P＜0.01). XiaXi Oral Liquid can maintain drug effect smooth and steady.1.3 Reversing left ventricular hypertrophy(LVH) effect: Xia Xi Oral Liquid can reduce theratio of LVW to BW by 24.05%(P＜0.01), decrease the left ventricular mass index.. It has asignificant statistic sense between the Xia Xi Oral Liquid treated group and the SHR placebogroup (P＜0.01).Which indicates Xia Xi Oral Liquid can reverse LVH to a degree.1.4 Morphology observation on target organ damages: Xia Xi Oral Liquid obviouslyalleviates the hypertrophy of cardiac muscle, protect blood vessel endothelium and reduce theproliferation of smooth muscle in tunica media after administration 8 weeks. The resultsshowed Xia Xi Oral Liquid has the function of protecting to cardiovascular system in SHR.2 The influence of Xia Xi Oral Liquid to neuron-endocrine-immune network. 2.1 The influence of Xia Xi Oral Liquid to the density of catecholamine(CA) in plasmaStudying the density of CA in plasma in SHR with Enzyme-Linked ImmunosorbnentAssay (ELISA). The results showed that the density of plasma CA in SHR is obviously higherthan that in WKY rats(P＜0.01). Xia Xi Oral Liquid can reduce the lever of plasma CA inSHR, which had a significant statistic sense in comparing with placebo group afteradministration 20 days(P＜0.01),and can reduce the lever to normal standard after 40 days(P＜0.01), the tendency lasting for 60 days. The result showed that Xia Xi Oral Liquid has thefunction of reduce the density of CA in plasma in SHR. and increasing catecholaminestandard may be the inner basis for excitement, hyperactive liver-yang ascending andhypertension in SHR. Xia Xi Oral Liquid can depress the activity of sympathetic nerve, lowerblood pressure and improve behavior and general condition through reduce the density ofserum CA in SHR.2.2 The influence of Xia Xi Oral Liquid to Tumor necrosis factor-α(TNF-α) density inplasma and intercellular adhesion molecule-1 (ICAM-1) expression in kidney arteriole in SHRStudying the density of TNF-αin serum with radio-immunity(RIA)and ICAM-1expression in arteriole with immunohistochemistery (ICH). The results showed that Xia XiOral Liquid can reduce TNF-αdensity in serum and ICAM-1 expression in arteriole in SHR.The results revealed that the injury of endothelial cell induced by higher expression in TNF-αand ICAM-1 may be one of the pathology basis about damage of superficial venues andlymph vessels by poison. And XiaXi Oral Liquid can alleviate the injury of endothelial cellthrough reduce ICAM-1 expression in SHR so that educe the function of removing toxicityand obstruction.2.3 The effect ofXia Xi Oral Liquid to rennin-angiotensin system (RAS)Studying the plasma rennin activity(PRA) and plasma angiotensinâ…¡(Angâ…¡) ontent withradio-immunity, serum Angi0tensin-Converting enzyme (ACE)activity withspectrophotometer, angiotensinâ…¡receptor typeâ… (AT₁R) expression in cardiac muscle withimmunohistochemistery (ICH) and the gene expressive content of AT₁R in cardiac musclewith Reverse Transcription -polymerasechain reaction(RT-PCR)in rats. The results showedthat Xia Xi Oral Liquid can reduce PRA, Angâ…¡content, ACE activity, AT1R expression andthe gene expressive content of AT₁R in cardiac muscle. It revealed that Xia Xi Oral Liquid has the function of lower blood pressure as Angiotensin-Converting enzyme inhibitor(ACEI)andangiotensin receptor block(ARB).Conclusion: SHR occur the abnormity of neuron-endocrine-immune network. Xia XiOral Liquid could lower blood pressure, reverse left ventricular hypertrophy, and improve targetorgan damages through a multiple composition, multiple pathways, multiple links andmultiple targets. Recover the function of neuron-endocrine-immune network.