The Research On The Flower Of Lonicera Macranthoides Volatile Organic Compounds And The Ecological Health Effects

Lonicera macranthoides Hand.-Mazz is an aromatic medicinal plant. Studies on the Lonicera macranthoides mainly focus on the analysis of its chemical composition, calculation of relative contents and ant-inflammatory. Few scholars emphasize on the influence of environmental factors to the plant volatile organic compounds during the extraction process and the development on the effect of ecological health. The research is based on the extract condition as the entry point to study the supercritical CO2extraction methods and its application in tourism. The results come out as follows:1. Study the extraction rate while pressure, temperature, CO2flow rate, time as test samples. The optimal process to extraction macranthoides flower using supercritical CO2is:pressure,28.5MP; temperature:38℃; time:2h; CO2flow rate:18kg/h. The affect size of extraction rate is:pressure>temperature> time>CO2flow rate.2. A total of45components were detected after GC-MS analysis to the volatile organic compounds of lonicera macranthoides flower. There are10kinds of hydrocarbons(44.03%), eight kinds of acids(24.14%),nine kinds of alcohols(6.34%),nine kinds of esters(8.452%) and six kinds of ketones(8.85%).Of which22showed higher than1%content, and the maximum content compounds-palmitic acid(12.34%), the total content including nonadecane(9.56%), nonacosane(8.95%),32alkyl(6.68%), pentadecane(5.23%) are over35.10%.3. Results indicated that the macranthoides flower volatile organic compounds can significantly inhibit the enhancement of the mouse peritoneal capillary permeability induced by acetic acid, Good resist effects to the xylene-induced mouse ear swelling,the carrageenan induced rat paw edema and rat pleurisy. To the cotton ball granuloma in mice model of chronic inflammation, the macranthoides flower volatile organic compounds can not significantly reduce granulation weight, but has a certain inhibitory trend, and large dose group can obviously reduce granulation index. It has an obviously effect to reduce the PGE2and MDA from the adrenal gland of rat carrageenin paw edema foot.It can significantly reduce the volume of NO and PGE2released from the pleurisy with effusion. 4. Results indicated that the macranthoides flower volatile organic compounds have antibacterial effects to all tested strains. The antibacterial effects are:gram-positive bacteria> gram-negative bacteria>fungi. The lowest antibacterial concentrations are:staphylococcus aureus(0.03125mg/mL),sarcina lutea (0.03125mg/mL),bacillus thuringiensis(0.03125mg/mL),bacillus subtilis (0.0625mg/mL), staphylococcus saprophyticus(0.25mg/mL), e. coli (0.5mg/mL), aspergillus(4mg/mL), candida albicans,pseudomonas aeruginosa,rhizopus oryzae are8mg/mL.Results also indicated that the staphylococcus aureus,sarcina lutea,bacillus thuringiensis can't grow at any culture temperature; The staphylococcus saprophyticus can't grow under22℃,will grow slightly at24-28℃,can't grow over30℃; The aspergillus,rhizopus oryzae,candida albicans will grow at22-26℃,will be inhabited absolutely under28-42℃; The e. Coli,pseudomonas aeruginosa can't grow under22℃,will grow the most productively at30℃,will be inhabited absolutely and can't grow over36℃.All the tested strains can't grow before10h in cultured.5. The lonicera macranthoides flower volatile organic compounds have the best antibacterial effects when concentration is3mg/mL, temperature25℃, time30min, inhibition rate reached67.4%.The bacterial inhibition rate in unmanned condition is63-86%,In manned sate is48-53%;The fungal inhibition rate in unmanned is20-42%, In manned sate is14-39%; The actinomycetes bacteriostatic rate in unmanned condition is50%in the rear, In manned sate is33%in the middle and25%in the back. The antibacterial effects are:gram-positive bacteria> fungi> actinomycetes.6. Results indicated that the lonicera macranthoides flower volatile organic compounds have positive effects to ease the anxiety, improve and adjust the mood of the people by testing changes of blood pressure and heart rate, testing the SCL-90scale, PSQI questionnaire survey,STAI scale questionnaire analysis, verified the effects to human longevity and health care through the social investigation.Conclusion:The Lonicera macranthoides flower volatile organic compounds have good effects to anti-inflammatory and antibacterial, Can be easily used in pharmaceutical, tourism, ecological health and other production areas of life...