Expressions Of Usp22 And Their Clinical Signi Ficance In Transitional Cell Carcinoma Of Hunman Bladder

Objective:To investigate ubiquitin specific peptide enzyme 22 in bladder transitional cell carcinoma of the gene and tissue and cell protein expression, and analysis of bladder cancer in different malignant degrees in tissues and cells, and in combination with the express differences clinical information, discuss the possible causality, and a conclusion that come from the correlation significanceMethods:Analysis collected from December 2008 to 2009 May huazhong university of science and technology affiliated xiehe hospital uropoiesis surgical resection of the tumor specimen bladder surgery, the 43 cases were malignant degrees and pathologic stage through pathologic reports prove correct, normal bladder tissue, based on cancer in 31 cases of distant normal mucosa tissue by 30 people, including male patients, women were 13 people, around the age between 41-77 years old, average age 57.6 years, draw materials are obviously the naked eye can identify the cancerous tissue, and stay away from the tumor, prevent the cut edge caused by high temperature electrotome surrounding tissue degeneration, patients undergoing surgery were not accepting radiotherapy before or chemotherapy. According to its malignant degrees grading divided into four groups; And central lab save nays EJ and bladder BIU eighty-seven lines, extraction its RNA and protein, press extraction source different classification, then after a retrovirus and real-time quantitatie polymerase chain reaction testing different type specimens and cells usp22 expression; Through the test specimen immunohistochemistry methods were used usp22 protein expression; Observe different categories of data with differences and specimens, combined with clinical data relevant analysis.Results:ubiquitin specific peptide enzyme22 in normal bladder tissue mRNA expressions is very low, quantity expressed in transitional cell carcinoma increased significantly, has the remarkable difference (p<0.01), in the organization of low-grade malignant lower than its expression in the organization highly malignant express (p<0.05). Immunohistochemistry display the positive rate in BTCC samples was 87.5%for 22 (36/43), higher than normal bladder mucous membrane tissues 35.4%(11/31), p<0.05, and with the increase of malignant degrees, positive elevated. But has nothing to do with factors such as age and gender (p>0.05). In the live bladder cancer cell lines, it have similar expression, EJ cells mRNA has higher expression biu-87 cellsConclusion:ubiquitin specific peptide enzyme 22 as a potential cancer stem cells marker, may raise the expression of some bladder tissue gene, resulting in the malignant transformation, normal cells in the bladder cancer occurrence and development process may play an important role; The expression in bladder tissue with malignant degrees or classification, is expected to be a positive correlation judgment illness development, while useful indexes are most likely to become the early diagnosis of tumors, and become an important index of a developed antitumor drugs available targets. But further studies are needed to explain its mechanism...