Study On The Related Controlling Matter And Signal Path Of ERK With Pathophysiological Methods To Research The Pathological Changes From Lung To Large Intestine

Objective:Observed pulmonary disease (allergic asthma) the impact of the large intestine in rats to explore the "lung and intestinal" mechanisms of pathological changes and related control material and ERK signaling pathways.Methods:Male Wistar rats were used, a blank group, lung (allergic asthma) group 2 group. In addition to the control group, the lung disease group with 4 points abdominal injection with 1% egg albumin, aluminum hydroxide, saline gel preparation of sensitization, after injection of 1% OVA inhalation ultrasound induced asthma. Pulmonary function test animals a week after atomization; collection of bronchoalveolar lavage fluid and lung intestinal microflora stool; collecting animals and femoral artery blood test blood TNF-α, IL-1; light microscopy lung, stomach, duodenum, Jejunum, ileum, colon, rectum pathologic changes; electron microscopy of lung and colon; immunohistochemistry lung, stomach, duodenum, jejunum, ileum, colon, rectum tissue CCK8, CGRP, SP, VIP Content; RT-PCR detection of lung, colon tissue iNOS, ERK mRNA expression.Results:1. PathophysiologyLung functions:lung disease group were significantly increased respiratory frequency, tidal volume, minute ventilation decreased significantly compared with the control group significantly (P＜0.01);Stool, texture, wet and dry weight observed:lung solution will reduce the number of rats, defecation difficult than the control group, the appearance of feces smaller, dry and hard texture, grain number reduction, reducing both wet and dry weight (P＜0.05);Gastric emptying and intestinal propulsion test:compared with the control group, lung rats increased gastric residual rate (P＜0.05), the end of the colon promote the rate of carbon reduction (P＜0.05);Pulmonary intestinal microflora:aerobic pulmonary tuberculosis group, fungi increased significantly, anaerobic bacteria were significantly reduced (P＜0.01); intestinal aerobic bacteria, fungi, E. coli was significantly increased compared with the control group (P＜0.01), And anaerobes, Bacteroides and Bifidobacterium were significantly reduced (P＜0.01);Morphological observations:Light microscopy showed that the modeling rat lung and colon before and after a certain pathological changes, and the duodenum, jejunum, ileum, rectum, no significant changes in gastric tissue; electron microscope showed that the lung and colon Pathological changes of a certain organization.2. The relevant regulatorsSP, VIP, CGRP, CCK8 content:content of lung reduction in lung tissue CCK8, CGRP levels increased, SP content increased, VIP content decreased, compared with the control group were significantly different (P＜0.01), colon CCK8 content increased, Increased CGRP content, SP content decreased, VIP content increased, compared with the control group were significantly different (P＜0.01 or P＜0.05), while the two groups of duodenum, jejunum, ileum, rectum and stomach the expression of VIP No significant difference between the amount (P> 0.05)TNF-α, IL-1:lung serum TNF-α, IL-1 significantly increased compared with the control group significantly (P＜0.01);iNOS mRNA expression:lung tissue of rats lung and colon tissue expression of iNOS mRNA increased compared with the control group significantly (P＜0.01).ERK mRNA expression:pulmonary disease rats lung, colon ERK mRNA increased expression differences compared with the control group (P＜0.01 or P＜0.05).Conclusion:1. Lung under certain pathological conditions and can affect the colon, colonic pathological changes occur.2. Lung under certain pathological conditions and can affect the large intestine, large intestine appears related to changes in regulation of substances.3. Lung under certain pathological conditions and can affect the colon, there ERK mRNA expression in the large intestine.4. CCK8, CGRP, SP, VIP, TNF-α, IL-1, iNOS may be a "lung and intestine, "the material basis.