The Influence Of BTJ About The Expressions Of P53 And C-myc Protein Of Lewis Lung Cancer Mice

ObjectsToday,Lung cancer is the most common malignant tumor in the world. It is the most threatening diseases to human health and life as well as the leading cause of cancer death, the experimental study based on the theory of Traditional Chinese Medicine, combining Medern Traditional Chinese Medicine search results and our clinic experience, Discussion on the TCM mechanism of lung cancer. And The experimental study observed the effect of BTJ rule to quality of life, Hemorheology, histomorphology, c-myc Protein and p53 protein of Lewis lung cancer, exploring it's mechanisms, which is to guide the treatment.Theoretical viewpointsIn this article,throngh discussing on the characteristic and function of the lung and the relations of lung cancer and the other four intenal organs, combining Modern TCM search results and our clinic experience,we think, the emphasis is on the weakness of lung-qi, pathogenic factor invade, lung-qi disorder, phlegm, blood stasis pyretic toxicity obstructed in Lung collaterals, then produced cancer poison, the basic rule is "bufeitongluojiedu"Experiental studyMethodologysTo establish lewis lung-cancer tumor-bearing mice model,we observde innibition ratio of high and low does of BTJ and CP. To analyze effect of BTJ and CP on improve tumor-bearing mice's quality life. We studied the effect of BTJ and CP on hemorheology of tumor-bearing mice by detecting peripheral blood. HE staining was used to observe the effect of BTJ and CP on the morphological and ultrastructural structure, flow cytometry test were used to explore the effect of BTJ and CP on cell apoptosis and the cell cycle block; immunohistochermical techniques were to detect the c-myc and p53 protein of lung cancer cell and analysised the effect of BTJ and CP on related tumor-produced gene protein.Results1. the inbibition ratio of high dose of BTJ is 35%, low is 21 %, CP is 46.9%. It has obvious dose-effect relationship between the BTJ. The inhibition ratio of high dose is obviously (P<0.05); The inhibition ratio of CP is obviously(P<0.01).2.Malignant tumor and CP both could low the food and activity of mice, its hair becam dull in colour and lose, lose weight, but BTJ can relieve the damage of Malignant tumor to body. The weight of mice between the modle group and CHM groups had no differences (P>0.05). but respectively comparing with CP group had obvious defferences (P<0.01). the weight of mice in modle group is obviously lower the normal (P<0.05).3. Blood viscosity (BV), plasma viscosity (PV), hematocrit (Hct),and red cell aggregation (RCA) of the high does of BTJ had obvious defferences with the molde group. (P<0.01).4. Lung cancer tissues of mice correspond with pathological change of cancer cell by light and electron microscopy respectively,which had obvious improvment in BTJ and CP.5. BTJ high dose,low dose were obviously higher than those of model group in the ratio of G0/G1cell (P<0.01), espescially BTJ low dose. meanswhile the experience showed that apoptosis rate of BTJ high dose,low dose and CP were obviously higher than those of model group(P<0.01), BTJ low dose were higher than CP in apoptosis rate(P <0.05), but BTJ high dose were lower than CP n apoptosis rate(P <0.05).6. Expressions of p53 protein in lung cancer tissues of Lweis mice were obviously higher than that in normal group, BTJ can lower the expressions.7. Expressions of c-myc protein in lung cancer tissues of Lweis mice were obviously higher. But the expressions lower of the high dose of BTJ is most obvious by treatment.Conclusions1. Both two doses of BTJ have inbititory action on lung cancer tumor-bearing mice. It has obviously dose-effect relationship. The dirct cytotoxic effect of BTJ on tumor cell inferior to CP.2. BTJH can relieve the damage of Malignant tumor to body. Meanwhile, it also can whet its appetite, restore its active function, improve its quality of life.3. BTJH can suppress amplification and metastsis of tumor by improving index of hemorheology.4. From microscopic angle we confirm the successful regulation of CP and BTJ to apoptosis, lower the proportion in S phase cell, inhibit growth,led to the lewis cell in G1 phase end, improve its apoptosis rate, then realize to inhibit cancer, in which BTJ low dose is most.5. BTJ and CP both have some Anti-tumor Effects, which mechanisms maybe have relations with improving the expressions of p53 and inhibiting c-myc protein in lung cancer tissues.