Investigation The Associativity In Xenotropic Murine Leukemia Virus-Related Virus(XMRV)and Prostate Cancer In Chinese Patients

ObjectiveTo establish a pathology tissue library of prostate disease of Chinese people the target in1000cases; To survey the epidemiological situation of XMRV infection in Chinese prostate cancer patients, To analyze the relationship between XMRV infection and pathogenesy of prostate cancer. Analyze the correlation of the XMRV and prostate disease.MethodsTo collect patients' blood specimens, to collect the patient's blood DNA, RNA specimens. To establish the blood specimen bank of Prostate cancer and benign prostatic hyperplasia patients. Collect patients' prostate tissues with transperineal prostate biopsy system and establish prostate tissue library. Detection124cases of prostate tissue specimens with Nested PCR and immunohistochemical for XMRV DNA and viral antigens in tissue. Analyze the relationship of XMRV and prostate cancer in clinical stage and pathological grade by statistical methods.ResultsWe had established initially the prostate disease pathology tissue library in Chinese people. Prostate pathologic specimens were more than300cases. The results of Nested PCR:68.5%patients had infected XMRV in total,73.4%prostate cancer patients had infected XMRV, and60.0%benign prostatic hyperplasia patients had infected XMRV. We detected XMRV antibody expression in prostate tissue by immunohistochemical methods, and the total positive response rate was32.35%in total; the prostate patients group's infection rate was43.04%; benign prostatic hyperplasia group's infection rate was13.33%.Conclusions We established the Chinese people prostate disease pathology tissue library system successfully and the number of samples has increased steadily. This laied the foundation for the futher studies prostate disease. Approximately43.04%Prostate cancer patients infected XMRV and about13.33%benign prostatic hyperplasia patients infected XMRV. There is some correlation between the XMRV infection and prostate cancer's pathogenesy. ObjectiveTo investigate the characteristics of transurethral partial cystectomy with a2μm laser in diagnosis and treatment for the bladder submucosal lesions in adults; To analyze the clinical characteristics of this method; To observe the efficiency of2μm continuous wave laser on the edge of the surgery field; To summarize and analyze the clinic characteristics of bladder tumor patients in follow-up periods after the surgery.MethodsFrom August2009to December2011, according to the established standard, we chose patients with bladder submucosal lesions treated by2μm laser via transurethral under sacral block. We overview these following variables: operation characteristics, clinic curative effect, pathologic diagnosis;From Apirl2009to Apirl2011, according to the established standard, we chose107patients with bladder carcinoma treated by2μm laser and summarize and analyze the clinic characteristics of bladder tumor patients in follow-up periods after the surgery for3years.ResultsAll operations were successful. Mean operative time was36.4minutes (range25to47), Perioperative blood loss was minimal. There was no obturator nerve reflection and no hemorrhage was detected after surgery. Postoperative pathological diagnosis:leiomyoma in3cases, pheochromocytoma in3cases, endometriosis in1case and metastatic bladder cancer in2cases. When the entire tumor and the full-thickness bladder wall at the base were freed, blood pressure of the pheochromocytoma patients reverted to stability.The follow-up periods of bladder carcinoma patients were between6to49months, and the median period was35months. There was no recurrence in situ. The survival rate is100%.Conclusions The2μm laser not only can vaporize and cut the wall of bladder finely, but also can dissect the muscular layers from connective tissue efficiently. So it can be partial cystectomy in the treatment of bladder tumor. It can be used in diagnose and treat bladder submucosal lesions. Patients could get accurate pathological diagnosis without further painful and some bladder tumors can be treated by minimally invasive surgery.For three yeas follow up periods, minireal influences on the patient's quality of life. It accords with the principle of evidence-based medicine.