The Interventions Of Yang Xin Tang On The Serum MiRNA-1and Urine Metabolomics Of Patients With Ventricular Premature Beats

Objective:Through investigating the clinical efficancy of Yang Xin Tang to the patients with ventricular premature beats in Syndrome of asthenia of Qi and blood and effects to serum miRNA-1and urine metabolomics of patients with ventricular premature beats,preliminary study the pathogenesis of patients with ventricular premature beats and explore its metabolomics markers.Methods:In this study,100patients with ventricular premature beats were randomly divided into treatment and control groups,and four weeks of a course of treatment,observing TCM symptoms before and after treatment,24h ventricular premature beats, and QT dispersion changes. The patients from the first part were randomly selected treatment group and control group10cases,determinating serum miRNA-1before and after treatment. The patients from the first part randomly selected treatment group and control group10cases, and the other10cases were selected from healthy volunteers,observing ventricular premature contraction in patients with medication changes in the composition by using urinary metabolites before and after UPLC-TOF/MS metabolomics techniques, and looking for ventricular premature beats in patients with metabolic group markers by the use of principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal correction partial least squares discriminant analysis (OPLS-DA).Results:1. the treatment group to improve the total effective rate was89.79%, the control group was60.42%, and it was statistically significant compared with control group(P<0.05).2.the treatment group, ventricular premature beats control the total effective rate was79.59%,in the control group of ventricular premature beats treatment rate of62.5%compared with the control group a significant effect (P<0.05).3.the treatment group after treatment, TCM syndrome score decreased significantly (P<0.01), the control group after treatment, TCM syndromes integral is also a downward trend and treatment, P<0.05, treatment and control groups after the treatment of TCM symptom scores decreased more significantly (P<0.01).4.treatment and control groups in the treatment of patients with QT dispersion have decreased significantly, with statistical significance, the treatment group after treatment, QT dispersion decreased compared with the control group is more significant, both statistically significant (P<0.05).5. treatment and control groups before treatment miRNA-1level higher expression, in varying degrees after treatment decreased (P<0.05). The treatment group after treatment of miRNA-1decreased more significantly compared with the control group after treatment(P<0.05).6.Urine metabolomics studies have shown that:the tryptophan in the urine of patients with ventricular premature beats, carboxylic esters, acyl-coenzyme A dehydrogenase, isovaleric acid was significantly reduced than the normal group.after treatment, the treatment group was increased more obviously than control group.Conclusion:1.Yang Xin Tang can significantly improve clinical sympto-ms in patients with ventricular premature beats.2.Yang Xin Tang can significantly reduce the incidence of premature ventricular beats and shorten the QT dispersion in patients with ventricular premature beats.3.Yang Xin Tang can significantly reduce the level of serum of miRNA-1of patients with ventricular premature beats.5. Yang Xin Tang can interfere with some urine metabolomics markers in patients with ventricular premature beats.