Tang Xin Ning Treatment Of Diabetic Heart Disease, Clinical Research And Basic Molecular Biology

The paper systematically studied the treatment of diabetic cardiopathy using the traditional Chinese medicine from five aspects: shicheng, review, theoretical, clinical and experimental studies.In the part of shicheng study, this paper summarized tutor professor L iiRenhe's main theoretical thought and experience in clinical diagnosis and treatment of Diabetes Mellitus and complications. In the meantime, the paper summed up tutor professor Wei Zhizhen' s main theoretical thought and clinical experience in treatment of diabetic cardiopathy using traditional medical theory.In the part of review study, the paper reviewed and evaluated all traditional research on diabetic cardiopathy : etiology, pathogenesis, clinical diagnosis criterionet cl. For modern medicine research, the paper summarized the studies on diabetic cardiopathy, including epidemiological data, etiology, pathogenesis, clinical diagnosis, laboratory examination and treatment.In the part of theoretical study, the paper summarized and induced all the ancient theories of traditional Chinese medicine about diabetic cardiopathy, including etiology, pathogenesis and therapeutic principles, et cl.In the part of clinical research, 30 patients with diabetic cardiopathy complicating with CHD treated by Tangxinning (TXN) were observed, another 30 patients with same condition treated by Lunanxinkang were compared randomly as the control group. Two group patients were studied in contrast with the aspects of general data, clinical symptoms, blood glucose, blood lipid, ECG et cl. The results indicated that TXN can obviously relieve the clinical symptoms such as angina pectoris, and enhance the pa t lent s' quality of life, it is superior to the control group. All the data show that TXN has a good treatment for diabetic cardiopathy. Through the analysis of clinical examination, the mechanism of treatment is related to decreasing blood glucose, adjustijng lipid metabolism disorder, improving the blood support of cardiac muscle and enhancing the function of heart.In the part of experimental study, using molecular biologic methods, the paper studied the TXN's mechanism of the prevention foratherosclerosis and the treatment of diabetic cardiopathy. We detected the cell proliferation, the expression level of platelet-derived growth factor (PDGF) , the expression of C-myc and C-fox gene by MTT, radioiramunoassay (RIA) and Northern blotting methods after smooth muscle cell stimulated by high glucose or high glucose and ET-1 in vitro at different time points. Compared with non-drug serum control groups, results demonstrate that TXN has inhibitory effects of the proliferation of vascular smooth muscle cells, and it can suppress the synthesization of PDGF and expression of C-myc, C-fos gene induced by ET-1 and high glucose. These results suggest that TXN is a potent, Chinese medicine with major anti- proliferative properties that may provide protection against atherosclerosis in diabetic cardiopathy. this property is one of main treatment mechanisms of TXN .