Transfer In Vivo Vascular Suture Experimental Study Of The Feasibility Of Gene Method To Explore And Urokinase Gene In Vascular Anastomosis

The possibility of gene transfer into arteries in vivo by stitching medical suture containing plasmid DNA. and the preventive effect of gene therapy with pro- uk gene transfered by this new method on the formation of anatomotic thrombus and restenosis were studied first time in this experiment.In the research of the possibility of gene transfer into arteries in vivo by stitching medical suture containing plasmid DNA, Wistar rats were randomly divided into control and treatment groups. Plasmid DNA was dissolved in PBS containing 5% sucrose. In control group, medical suture was soaked in the pN₂-plasmid solution for 72 hours before stitched. In treatment group, medical suture was soaked in the pN₂-LacZ plasmid solution. By means of Southern blot analysis. 3 - galactosidase activity assay and histochemical staining, the following results were shown: when isolated DNA from stitched arteries on 2th,7th, 90th day following operation were hybridized with the radiolabeled LacZ probe, bands appeared in theatment group, but didn't appear in control group. β-galactosidase activity couldn't be detected in control group. In treatment group, β-galactosidase activity could be detected on 2th day following operation. It got the maximal level on 7th day and was present for 3 monthes. Histochemical staining of arteries revealed blue( positive) in 100% arteries on both 2th and 7th day following operation, and 80% on 90th in treatment group but all negative in control group. Blue appeared not only in vascular smooth muscle cells but...