| 1. The establishment of isolated rat lung reperfusion modelThe isolated perfused lung model is simple, reliable and inexpensive, commonly used in small animals to investigate lung preservation and reperfusion injury after preservation and cold storage. Whole blood perfusion more closely mimics the in vivo physiologic situation, and is superior to constituted media. We developed a relatively less-invasive isolated perfused rat lung lung model. Methods: Angiocatheters were inserted into femoral artery and femoral vein of the recipient rat to establish the controlled cross-circulatory. The venous blood from femoral vein was pumped into the pulmonary artery of the ex vivo lung block after preservation and cold storage to provide inflow, and pulmonary effluent blood from the lung block was collected and returned to the support rat via the femoral artery for detoxification and deoxygenation. 36 donor rats were divided into 6 groups randomly, and the donor lungs were flushed and preserved with 4℃ EC solution for 8 h. Each support rat was used to sequentially perfuse two double-lung blocks. The inflow sample to each lung block was analyzed for pH, pO2, pCO2, and Hct to follow alterations in recipient rat physiology. Results: There were no statistical differences in the pH, PvO2, PvCO2 or Hct between groups (P>0.05) . Conclusions: The syngeneic support rat can delivers constant systemic venous blood at stable physiologic parameters to the ex vivo lung block.2. The influence of triphosphate-sensitive potassium-channel openers(PCOs) on donr lung preservationPCOs has been shown to protect organ against ischemia-reperfusion injury in a variety of experiments. We used Nicorandil, a nicotinamide nitrate with adenosine triphosphate-sensitive potassium (KATP) channel opener activity, at the induction of...
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