Test On The Hypothesis Of Blood Stasis Causing Wind Syndrome

Objective: Research the relationship between focal cerebral infarction and blood stasis, and study the effect and its mechanism on the focal cerebral infarction rats by promoting blood flow and stopping endogenous wind.Method: The model rats of focal cerebral infarction was established based on the principle of photochemistry initiation of thrombosis. Through observing the antagonistic effect and influence in different level on model rats by treat with Huoxue Xifeng Decoction (HXD) which can promote blood flow and stop endogenous wind. Observe the neurological symptoms through scoring, measure the cerebral infarction volume use TTC staining method, measure the water content of brain tissue with weighting method and Laser Doppler flowmetry was used to detect the regional cerebral blood flow(rCBF) in the ischemic core. Radio-immunity method was used to detect the contents of IL-1β,TNF-αand ET. Spectrophotometric method was used to detect the activities of SOD,GSH-PX, ATPase, LDH and the contents of MDA and NO. Pathomorphological changes was observed by HE staining method. Apoptosis of nerve cells was studied by TUNEL. The protein express of apoptosis related gene Bax,Bcl-2,Caspase-3 was observed by immunohistochemical method, the express of Caspase-3mRNA and VEGFmRNA was studied by RT-PCR. Result: Contrast to the model, HXD could obviously promote neurological deficit, decrease the water content of brain tissue and the volume of cerebral infarction zone,increase the rCBF, decrease the contents of IL-1βand TNF-α, regulate the ratio of ET/NO, promote the activities of SOD, GSH-PX, Na⁺-K⁺-ATPase, Ca²⁺-ATPase and LDH, decrease MDA content, decrease numbers of TUNEL masculine cells, up-regulation the express of Bcl-2 protein which can restrain apoptosis, down-regulation the express of Bax,Caspase-3 protein and Caspase-3mRNA which can hasten apoptosis and up-regulation the express of VEGFmRNA.Conclusion: The model rats of focal cerebral infarction which induced by photochemistry had stagnated blood signs, and the treatment of promoting blood flow and stopping endogenous wind has significant brain protective effect, the mechanism may be related to that it can increase the rCBF,restrain inflammatory reaction , regulate the blood vessel relax-contract condition ,improve energy metabolism and antagonize lesion of free radicals,regulate the express of apoptosis related gene and promote the blood vessel born newly.