Allicin, And Alprostadil Injection Prevention And Treatment Of Acute Myocardial Infarction Reperfusion No-reflow Experimental Research

Objective To evaluate and discuss the effects and mechanism of Garlicin injection and Lipo-PGE1 injection on myocardial no-reflow in a mini-swine model with acute myocardial infarction(AMI) and reperfusion and to research the related mechanism.Methods:The mini-swines were randomized into four study groups:six in sham-operated group,eight in control group,eight in Garlicin injection-treated group and eight in Lipo-PGE1 injection-treated group.The mini-swines in the latter three groups were subjected to the left anterior descending(LAD) coronary occlusion by dilated balloon for two hours and then reperfursion for three hours to make the no-refiow model after percutaneous transluminal coronary angioplasty.Before AMI, at two hours after AMI and at one,two,three hours after reperfusion,the data on hemodynamics was examined by means of the catheter,the blood serum and plasma were examined by the radio-immunity analysis and the area of no-reflow was respectively evaluated with the myocardial contrast echocardiography(MCE) in vivo and pathological staining means.After selecting the myocardial issue in different regions,the change on the adhesion molecules and other molecules related to the effects of endothelial cell was detected by relative quantitive real-time PCR, Western-blotting,immunohistochemistry and HE staining.Results:The areas of no-reflow region were reduced by the Garlicin injection and Lipo-PGE1 injection(P＜0.05) and the left ventricular systolic pressure(LVSP) and left ventricular end-diastolic pressure(LVEDP) were restored.The adhesion and aggregation of polymorphonuclear leucocyte and platelet were lessened to inhibit the ischemia/reperfursion injury and the thrombosis by lowering the mRNA expression of GM-CSF,PECAM-1,Intergrinβ1,VCAM-1,PDGF,E-selectin and P-selectin and the protein synthesis of PECAM-1,PDGF receptor and Intergrinβ1(P＜0.05).In addition, the mRNA expression and protein synthesis of VEGF and eNOS were upregulated (P＜0.05) by Garlicin injection and Lipo-PGE1 injection to ameliorate the function of endothelial cell in coronary capillary and product NO to dilate the capillary.Conclustions:The Garlicin injection and Lipo-PGE1 injection had exerted the certain effect on inhibitting the adhension of plathet and leukocyte and ameliorating the function of minicirculation to reduce the area of no-reflow and effectively provent and treat the no-reflow phenomenon after reperfusion treatment of the acute myocardial infarction.