Correlation Of The Polymorphism Of HLA Alleles With The Susceptibility Of Damp-heat Syndrome In Guangdong Han's Population

ObjectiveTo explore the correlation between HLA alleles polymorphism and the susceptibility of damp-heat syndrom,and to assess the related influencing factors of damp-heat constitution in Guangdong Ham' s population.Study on the genetic basis of syndrome focussed on relatively stable constitution and to specific population,will help to research the essence of syndrome deeeply and provide a new angle to discusse the mechanism of." the same syndrome of different disease".Methods1.By using cross-sectional study method,148 Ham' s people(over 18 years old,65 in damp-heat constitution group and 83 in normal group) in Guangdong province were investigated with questionaire to emphasize on the damp-heat constitution people' s diet and lifestyle and the general demography.We drew up the diagnostic standards of damp-heat constitution and the damp-heat constitution in Chinese Medicine Questionaire,by which the scores of the damp-heat constitution were measured.Single factor analysis and multiple stepwise regression were applied to analyze major influencing factors.2.The genotypes of HLA-A,B and DRB1 alleles were determined in the damp-heat constitution and the normal constitution group come from Guangdong province and non-Guangdong province respectively(15 cases in each group) by DNA chips.The phenotype frequencies of HLA alleles in each group were calculated by direct counting method.Fisher' s exact test was applied to analyze the diffrences of the phenotype frequencies of HLA alleles between two groups.Results1.As P<0.10,12 influencing factors entered into the regression equation by mutiple stepwise regression analysis,they were as follows in decreasing order:age,barbecue,cold drink,tobacco addiction,sweet food,body mass index(BMI),meat,sleeping early and getting up early,salted fish,spicy food, sour food,and irregular sleep.The scores of the damp-heat constitution were relatively higher in the individual prefered to cold drinking,barbecue,sweet food,meat,salted fish and smoking,elder,and the individual who having irregular sleep,while the scores were relatively lower in the individual who sleeping early and getting up early,and preferring to sour or spicy food.2.The phenotype frequency of HLA-A*02 in the damp-heat constitution group was lower than that in normal constitution group in Guangdong Han population(16.7%vs 50.0%,P=0.006).3.The phenotype frequencies of HLA-B*15,B*46 alleles in the damp-heat constitution group of the Han people in Guangdong were significantly lower than those in damp-heat constitution group in non-Guangdong Hans(3.3%vs 23.3%, P=0.035;3.3%vs 23.3%,P=0.035);the frequency of B*40 allele in the damp-heat constitution group of the Han people in Guangdong was higher than that of non-Guangdong Hans(30.0%vs 16.7%).4.Compared with the normal constitution group in Guangdong Han population,the phenotype frequencies of HLA-B*40 had tended upwards(30.0%vs 16.7%),while the phenotype frequencies of B*46,B*15,DRB1*09 had tended downwards(3.3%vs 20.0%;3.3%vs 16.7%;6.7%vs 16.7%) in the damp-heat constitution group of the Han people in Guangdong.Compared with the normal constitution group in non-Guangdong Han population,the phenotype frequencies of HLA-A*11,B*46,DRB1*08 had tended upwards(33.3%vs 16.7%;23.3%vs 13.3%; 20.0%vs 3.3%),while the phenotype frequencies of A*24,DRB1*09 had tended downwards(13.3%vs 30.0%;10.0%vs 23.3%)in the damp-heat constitution group of the Han people in non- Guangdong.Conclusion1.The eating habits of preferring to sweet food,meat,cold drinking, barbecue,salted fish and smoking,the living habits of irregular sleep,and obesity were the important factors in the development of damp-heat constitution in Guangdong Han population,lt is expectable to prevent the disease and promote the health via correting damp-heat constitution towards high-risk population by changing lifestyle and behavior.2.It suggests that HLA-A*02 might be the protective gene of damp-heat constitution in Guangdong Han population.3.The results indicate that there might be the different immunogenetics basis in the damp-heat constitution of Guangdong and non- Guangdong Han population.