The Theoretical And Experimental Study On Relation Between The Weiqi And The Nerve Conduction

Objective: Establish the Weiqi-deficiency rat model, choosethe Yiqigubiao therapy _Yupingfengsan to the rats in treatment orprevent way. Obseve the change on the rats in neuro-immune,neurotrophic, neurotransmitter after making model andadministration. According to experiment results, interpret therelation between Weiqi and the nerve conduction and define theconcrete links, preliminarily investigate the perhaps mechanismthat Weiqi realize its physiological functions based on nerveconduction. Prove the conduction of Weiqi with experimentalapproach, preliminary exploration the theory of Qimai, Enrich theWeiqi theory and collaterals theory of traditional Chinese medicine.Compareing the treat effect and the prevention effect ofYupingfengsan, discuss the influence the nerve conduction onsyndrome of Weiqi-deficiency caused by Yupingfengsan. Provideevidence for extending the clinical application of Yupingfengsan.Methods: theoretical research is base oncollaterals and Weiqitheory of Chinese traditional medicine. Explained the connotationof Weiqi. Analyzed the theory of nerve conduction and comparedbetween Weiqi and nerve conduction from three aspects: generation,operation and function. Demonstrated the correlation of Weiqi andnerve conduction.Experimental research: 72 SD rats, weight range from 200g to250g, were divided into 6 groups by random: normal group, Weixugroup, lower immune group, model prevention group, model treating group and lower immune treating group. Took the cold and hotalternate stimulate on Weixu group, model prevention group andmodel treating group. First the rats were placed into specially madecages, 6 rats in one cage, then placed the cage into the refrigeratorunder the -20℃. 20 minutes later, took out the cage at normaltemperature. During this cold stimulate, observed the rats each10mins. After 4 hours, placed the rats into 40℃incubator for 15mins. Cold and hot alternate stimulate on rats once a day for 6 days.Rats of lower immune group and lower immune treating group wereintraperitoneal injected lower dose cyclophosphamide with 10mg/kgof once a day for 6 days to make lower immune model. Rats took thephysiological saline by gavage for 7 days after making model innormal group, Weixu model group and lower immune group. Rats ofmodel prevention group were taken 1g/kg.d Yupingfengsan for 7 daysby garage from the first day making model. Rats of the model treatinggroup and lower immune treating group were taken 1g/kg, dYupingfengsan for 7 days by gavage from the last day making model.Take blood serum, sciatic nerve, brain issue, ventral skin.Measured IL-2 content in serum and NGF content in serum and sciaticnerve by Enzyme-linked Immunosorbent Assay, measured SP content inskin and brain and apoptosis in brain by immunohistochemicalmethod.Results: The serum IL-2 content of Weixu group is higher thannormal group with extremely significant difference (P＜0.01)through the comparison, and without significant differencecomparing with lower immune group(P＞0.05).It suggests that Weixugroup have changed in immune. The serum IL-2 content of modeltreating group and model prevention group are lower than Weixu groupwith extremely significant difference (P＜0.01). The serum IL-2content of model prevention group is lower than model treating groupwith extremely significant difference (P＜0.01). It suggests thatYupingfengsan has the treat effect on Weixu model and the prevention effect is better than treatment. The NGF content in serum of Weixugroup is higher than normal group, but without significantdifference. The NGF content in serum of model prevention groupexists extremely significant difference (P＜0.01) comparing withWeixu group, lower immune group and lower immune treating group, andexists significant difference(P＜0.05)comparing with normal group.The NGF content in serum of model treating group exists extremelysignificant difference(P＜0.01)comparing with Weixu group and lowerimmune group, and exists significant difference (P＜0.05) comparingwith normal group and lower immune treating group. The NGF contentin serum of model prevention group is lower than model treatinggroup, but without significant difference (P＞0.05). It suggeststhat treatment or prevention with Yupingfengsan has the effect ofdecrease the NGF content in serum of Weixu model. The change on NGFcontent in sciatic nerve does not similar to it in serum. The NGFcontent in sciatic nerve of Weixu group is lower than normal groupwith extremely significant difference (P＜0.01),and existssignificant difference (P＜0.05) comparing with model preventiongroup. It suggests that NGF content in sciatic nerve have deceasedin Weixu group, and this change can be hindered by prevention effectof Yupingfengsan. The SP positive staining area can be seen in clipsof abdominal skin of all groups. But the area of SP positive stainingof Weixu group and lower immune group are smaller than normal groupwith significant difference (P＜0.05). It suggests that Weixu couldcause the decrease of SP in skin and this change may associate withthe low immune. The number of SP positive cell in frontal cortexof Weixu group is smaller than normal group with extremelysignificant difference (P＜0.01). The number of SP positive cellof model prevention group and model treating group has significantdifference (P＜0.05) comparing with Weixu group. It suggests thatWeixu could cause the decrease of SP in frontal cortex, and theprevention effect and treatment effect of Yupingfengsan could increase the contant of SP in skin of Weixu group. The number ofSP positive cell in frontal cortex of lower immune group is smallerthan normal group. It suggests that the change of SP in frontalcortex may associate with the low immune. Apoptosis in frontalcortex positive staining area can be seen in brain clips of allgroups. The number of TUNEL positive cell of Weixu group is biggerthan normal group with extremely significant difference (P＜0.01).And model prevention group is smaller than Weixu group withsignificant difference(P＜0.05). It suggests that making Weixu modelpromote the apoptosis in frontal cortex, and this change can behindered by prevention effect of Yupingfengsan.Conclusion: Weixu model made by the cold and hot alternatestimulate have the character of lower immune, which is accord withthe characteristics of syndrome differentiation in traditionalChinese medicine. Weixu model rats change on nerve conduction;include the decreasing NGF content in sciatic, decreasing SP of skinand frontal cortex and increasing apoptosis in frontal cortex. Itsuggests that the syndrome of Weixu has the characters not only oflower immune, but also on nerve conduction. It proved that Weiqihave the function not only in immune but also in informationconduction. Weiqi is related with nerve conduction.Yupingfengsan has the treat effect on syndrome of Weixu throughincreasing immune, and improve the conduction-hinder in syndromeof Weixu by influencing NGF content in serum, SP content in skinand frontal cortex and apoptosis in frontal cortex. And preventive-useYupingfengsan has better effect than treat use.