Evaluation Of Rat Model With Syndrome Of Accumulation Of Cold Fluid In The Lung In Chronic Obstructive Pulmonary Disease

Objective: This thesis expounds systematically the theoretic problems about the syndrome of accumulation of cold fluid in the lung in chronic obstructive pulmonary disease(COPD) to enrich the theory of the syndrome. The rat model with the syndrome of accumulation of cold fluid in the lung in COPD was established by inhalation cigarette,exposure to cold and intratracheal instillation of lipopolysaccharide(LPS) .Based on the pathologic rat models, observed the microcosmic mechanism by the pathologic rat models. Function developing change and promptly effect of the accumulation of cold fluid syndrome in the lung in COPD by stomach irritation with"Yang-Warming and Fluid-Dispersing Formula"were provided the mechanism to provide the thinking and theory to prevent and heal it.Methods: This research based on the rat models with the accumulution of cold fluid syndrom in the lung in COPD was set up with the methods of fleding with ice water with cold property in winter temperature 0℃in halation cigarette and intratracheal instillations of LPS and treatel with"Yang-Warming and Fluid-Dispersing Formula". The observe the relevant indexes induding symptoms and signs pathological section of lung tissues,interleukin-8(IL-8) and interleukin-10(IL-10) in blood serum,MMP-9 and TIMP-1,and also the lung function measurements were carried out.Results: The results of the experiment showed that the development of pathologic rat models with the accumulation of cold fluid syndrome in the lung in COPD which proved to be repetitive is comparatively successful and reliable.The symptoms and signs of pathologic rat models with the accumulation of with clinical symptoms and signs of patients of the accumulation of cold fluid syndrome in the lung in COPD. The changes of microcosmic indexes coincided with those of COPD related to the syndrome. For example, the expressive index of avoirdupois were alleviated, dynamic lung compliance(Cldyn) decreased while inspiratory resistance(Ri) and expiratory resistance(Re) increased, lung tissues were inflammatorily damaged,IL-10 and IL-8 had no significant changes(P>0.05) comparing with the control group's.The expression level of MMP-9,TIMP-1 and the result of MMP-9 than TIMP-1 increase.The symptoms and signs of pathologic rat models didn't recover during the following 30 days by stoping the pathogeny.The remedy in the treated group ameliorated above indexes except IL-10 and IL-8 in blood serum,and the curative effect increased little by little.Conclusion: It is feasible to set up the rat models with the syndrome of accumulation of cold fluid in the lung in COPD which conformed to the case in clinical and can be developed the model standard.The mechanism of the accumulation of cold fluid syndrome in the lung in COPD may be increase the MMP-9 and TIMP-1 and disturb the balance of MMP-9/TIMP-1,especially the consequence of MMP-9 than TIMP-1.The symptoms and signs lung function and inflammatory reaction in airway of pathologic rat models could be a meliorared well by"Yang-Warming and Fluid-Dispersing Formula".It means that we should treat it early and insistently.