Model Establishment Of Acetic Acid Daub Ulcer Combined With Cold And Hot Syndrome And Study Of Cold And Hot Formula On Formula And Syndrome Correlation

Object:To estabilish acetic acid daub ulcer models with cold and hotsyndrome.Reveal the essential of acetic acid daub ulcer models with cold andhot syndrome.Observe the mechanism and regulation of Dahuanhuanglianxiexindecoction and Lizhongpill on Peptic Ulcer models with cold and hotsyndrome.Method:Use cold factor(cold water,0.3mol/L NaOH) and hot factor(8%peper powder,60% ethanol mixed solution) to establish cold and hotsymdrome model conbined with acetic acid daub ulcer model.1.Observe thegeneral state,gastric mucosa,urination and fecal amount.2.Detected theulcer index,the content of TNF-a.IL-8,ET,NO and INOS.3.Detected theEGF,TGF-a,SOD and MDA in the stomach tissue.4.Detected the content of5-HT and NE in the brain tissue.Results:The cold factor and the hot factor could aggravated the damagedegree.The content of SOD of the cold syndrome and the hot syndromedecreased.Compared to the blank group and other group,the content of MDAincreased in the hot syndrome.The content of NO decreased the coldsyndrome group and increased in the hot syndrome,the content of ETincreased in the cold syndrome.The content 5-HT increased in the coldsyndrome and the hot syndrome,but the cold syndrome's is moreobviously.the content NE increased more obviously in the hotsyndrome.Compared to the cold syndrome group,the content IL-8 and TNF-aincreased more obviously in hot syndrome.The content GAS increased in thecold syndrome and the hot syndrome,but the hot syndrome's is moreobviously.Compared to the blank group,the content of EGF and TGF-adecreased in the cold syndrome and the hot syndrome.There was nodifference between the two groups.Conclusion:There are differences among SOD,MDA,NO,ET,5-HT,NE,GAS,EGF and TGF-a many other aspects in Peptic Ulcer models with coldand hot syndrome.The mechanism of the cold formulae Dahuanhuanglianxiexindecoction and the hot formulae Lizhongpill embodied in differentaspects.The acetic acid daub ulcer model with cold and hot syndrome wassuccessful..