The Experimental And Clinical Study On Dahuang Zhe Chong Pill In Sequelae Of The Deep Venous Thrombosis

Objective:To observe the effect of Dahuang Zhechong Pill onthe related indexes and ultrastructure in the venous thrombosis modeland the damaged venous model in ratsand to observe, the thetherapeutic efficacy of Dahuang Zhechong Pill in sequela of the deepvenous thrombosis. Study the therapeutic mechanism of DahuangZhechong Pill on the patients with sequela of the deep venousthrombosis though the indexes.Research technique:â‘ Experimental Research:building thevenous thrombosis model though inferior vena cava ligation (the firstmodel group for short), simultaneously building the damaged venousmodel by the guidewires (the second model group for short). Twomodle groups divide randomly into six groups, including the lowdosage group of Dahuang Zhechong Pill, the medium dosage group ofDahuang Zhechong Pill, the high dosage group of Dahuang ZhechongPill, the control group, the model group and the blank group. Studythe effects of Dahuang Zhechong Pill on Indexes in two model groups,including the tissue plasminogen activator (t-PA), plasminogenactivator inhibitor-1(PAI-1), D-Dimer, endothelin(ET), nitric oxidesynthase (NOS), thromboxane B₂ (TXB₂,), 6-keto-prostaglandin F₁α(6-K-PGF₁α), P-selectin and intrastruction of venous specimen forlight microscope and electron microscopic examination,ect.â‘¡Clinical Research:to study the effects of Dahuang Zhechong Pill onrelated Indexes on the patients with the deep venous postthromboticsyndrome, for example: photoplethysmography , hemagglutination test,P-selectin, tissue plasminogen activator (t-PA), thromboxane B₂(TXB₂),â…§factor antigen (VWF-Ag), 6-keto-prostaglandin F₁α(6-K-PGF₁α),ect.Findings:1,Experimental Study With respects to related indexes and ultrastructure ,the studyshows that there was significant difference between the venousthrombosis model group and the damaged venous model group.â‘ Dahuang Zhechong Pill has regulatory effect in the content oft-PA of the first model group and the second model group. It also candecrease the contents of PAI-1 and D-Dimer of the first model groupand the second model group. There was significant differencebetween the first model groups and the model group(p＜0.05). Therewas significant difference between the second model group and themodel group(p＜0.05).There was no difference between the firstmodel group and the blank group (p＞0.05). There was no differencebetween the second model group and the blank group (p＞0.05).Therewas no difference between the first model group and the secondmodel group(p＞0.05).â‘¡Dahuang Zhechong Pill can increase the content of TNOS of thefirst model group and the second model group, but it can decrease thecontent of iNOS in both the first model group and the second modelgroup. There was significant difference between the first modelgroup and the model group(p＜0.05). There was significant differencebetween the second model group and the model group(p＜0.05). Therewas no difference between the blank group and the first group(p＞0.05). There was no difference between the blank group and thesecond group (p＞0.05). There was no difference between the firstmodel group and the second venous model group(p＜0.05).â‘¢Dahuang Zhechong Pill can increase the content of 6-K-PGF1_aof the first model group and the second group, but it can decrease thecontents of TXB₂, and ET in the first model group and the secondmodel group. There was significant difference between the firstmodel group and the model group(p＜0.05). There was significantdifference between the second model group and the model group(p＜0.05). There was no difference between the blank group andthe first group (p＞0.05). There was no difference between the blankgroup and the second group (p＞0.05). There was no differencebetween the first model group and the second venous modelgroup(p＜0.05).â‘£Dahuang Zhechong Pill has negative effect in the quantity ofP-selectin of the first model group and the second model group. Therewas significant difference between the first model groups and thecontrol group(p＜0.05). There was significant difference between thesecond model group and the control group(p＜0.05). There wassignificant difference between the first model groups and the modelgroup(p＜0.05). There was significant difference between the secondmodel group and the model group(p＜0.05).There was significantdifference between the first model groups and the blankgroup(p＜0.05). There was significant difference between the secondmodel group and the blank group(p＜0.05).⑤To study the intrastructions for light microscope examinationand electron microscopic examination,the study shows that DahuangZhechong Pill has an obvious inhibiting effect on thrombosis,and hasprotective effects on endothelial cells.2,Clinical StudyDahuang Zhechong Pill has positive remarkedly effects onrelated indexes. Though observing 32 example cases,the study showsthat symptoms and physical signs obvious change for the better afterthe treatment. The related indexes, such as PT, APTT, FIB, P-selectin,t-PA, TXB₂, 6-K-PGF₁α, VWF-Ag,ete,had improved. The results havethe significance after statistics processing.Conclusions:1.Experimental study shows that Dahuang Zhechong Pill canimproved the related indexes on the venous thrombosis model group and the damaged venous model group. Dahuang Zhechong Pill has anobvious inhibiting effect on thrombosis,and has protective effects onendothelial cells and vascular repair.2. Clinical study show that Dahuang Zhechong Pill is a kind ofeffective and reliable medicine for treating patients with DVTS. Thestudy is a new application of old herb prescription, and has providedthe science objective basis for Dahuang Zhechong Pill of clinicalapplication in angiopathy field.