| Objective: In this study, SD rats were committed to swim exhaust + hunger + injection of adrenaline caused QDBS model was classic recipe Dahuang zhechong pill its P- selectin(GMP-140, P-selectin), tissue fibrinolysis, plasminogen activator inhibitor(PAI), 6- keto plasminogen activator(t-PA)- prostaglandin F1α(6-Keto PGF1α), thromboxane(TXB2) and blood rheology(Hemorheological) impact. Discussion Dahuang zhechong pill bug experimental platelet activation and mechanisms related factors and blood stasis rats provide a theoretical basis for clinical application.Method:1 Choice of body weight 230 ± 10 g healthy male SD rats were 40, one week after adaptive feeding randomly divided into five groups of eight, namely: Dahuang zhechong pill low dose group(A group), Dahuang zhechong pill high dose group(group B), the control group(group C), Salvia positive control group(group D) and the model group(M group).2 Model Preparation: 40 male SD rats, in addition to the control group, the other rats eat during modeling are limited to the usual 2/3. Daily Weigh weight, were fed. Dahuang zhechong pill daily low-dose group Dahuang zhechong pill(0.7g / kg.d) suspension gavage, Dahuang zhechong pill daily high-dose group Dahuang zhechong pill(1.4g / kg.d) suspension gavage daily with compound Dan SM group(1.0g / kg.d) suspension gavage, the control group and the untreated group daily with normal saline(10ml / kg.d), continuous irrigation stomach 7 days. In addition to the control group were not swimming, the rest of the group a day at room temperature 22 ℃, 50 cm × 50 cm deep bucket swim to exhaustion.3 Experimental observation: Above operation for seven consecutive days, fasted water, femoral artery blood, measured P- selectin(GMP-140), t-PA(tissue plasminogen activator), PAI(plasminogen activator inhibitor), 6-keto-prostaglandin F1α(6-Keto PGF1α), thromboxane(TXB2) and blood rheology.Results:1 Dahuang zhechong pill on experimental rats with blood stasis PselectinThe results showed that the model group compared with the control group, P- select rat hormone increased, statistically significant differences(P<0.01), indicating a successful replication of blood stasis rats; Dahuang zhechong pill low and high dose compared with model group, rats P- selectin significantly lower statistically significant difference(P<0.01); Dahuang zhechong pill low, compared to, P-choose between high-dose group and high dose group Su reduce more obvious advantage than the low-dose group, but not statistically significant(P>0.05); SM group compared with the control group, there are statistically significant(P<0.01); Dahuang zhechong pill low, high-dose group compared with the control group, a statistically significant difference(P <0.01); Dahuang zhechong pill low and high dose groups compared with salvia, Dahuang zhechong pill low and high dose group than the SM group, statistics significance was significantly(P<0.01). Show Dahuang zhechong pill can reduce insect P- selectin levels, improve blood stasis, and better than the SM group, and the high-dose group than the low dose group trend.2 Dahuang zhechong pill on experimental rats with blood stasis t-PA and PAIThe results showed that the model group compared with the control group, the rats reduced plasma t-PA, PAI increased, statistically significant differences(P<0.01), while blood stasis showed lower plasma t-PA, PAI increased; Dahuang zhechong pill low and high dose group compared with the model group, the rats increased t-PA, PAI decreased statistically significant difference(P<0.01); Dahuang zhechong pill low phase between the high-dose group than the high dose group no significant increasing trend was not statistically significant(P>0.05); Salvia group compared with the control group, t-PA was not statistically significant(P>0.05), PAI increased significantly statistics significance(P<0.01); Dahuang zhechong pill low dose group compared with the control group, PAI increased, a significant difference(P<0.05), t-PA decreased, there was statistically significant(P<0.01); Dahuang zhechong pill high dose group compared with the control group, PAI increased, t-PA decreased, there was statistically significant(P<0.01); Dahuang zhechong pill low and high dose groups compared with the SM group, no statistically significant(P> 0.05). Show Dahuang zhechong pill can be increased t-PA, PAI lower levels, improve blood stasis.3 Dahuang zhechong pill affects on blood stasis rats and TXB2 of 6-Ke to PGF1αThe results showed that the model group compared with the control group, rats were elevated plasma TXB2, 6-Keto PGF1α reduced. 6-Keto PGF1α reduced statistical differences(P<0.05), TXB2 increasing trend but no statistical significance(P>0.05), showed that reducing blood stasis occurs when the body’s 6-Keto PGF1α expression; Dahuang zhechong pill with low-dose group model group rats significantly increased plasma 6-Keto PGF1α Statistical differences(P<0.05), TXB2 no significant difference(P>0.05); Dahuang zhechong pill high dose group compared with the model group, 6-Keto PGF1α increased more significantly, there is a statistically significant difference(P<0.01), TXB2 tended to decrease, but not statistically significant; Dahuang zhechong pill low, compared to the high-dose group liter 6-Keto PGF1α between the high-dose group Gauguin was significantly better than the low-dose group, and there was statistically significant(P<0.05), TXB2 tended to decrease, but not statistically significant(P>0.05); Dahuang zhechong pill low dose group compared with the control group, 6-Keto PGF1α, no significant difference(P>0.05), TXB2 there is an increasing tendency but not statistically significant(P>0.05); high-dose group compared with the control group, 6-Keto PGF1α increased statistically significant(P<0.05), TXB2 was no significant difference(P>0.05); SM group and blank control group, 6-Keto PGF1α not statistically significant(P> 0.05), TXB2 elevated but not statistically significant trend; Dahuang zhechong pill low dose group compared with the SM group, TXB2 and 6-Keto PGF1α no significant change was not statistically significant(P>0.05); high-dose group compared with the SM group, TXB2 tended to decrease, but not statistically significant(P>0.05), 6-Keto PGF1α increased more significantly, there are statistically significant(P<0.05). Dahuang zhechong pill show high-dose group had lower TXB2 trend can be elevated 6-Keto PGF1α expression, Adjustable 6-Keto PGF1α / TXB2 balance and high-dose group than in the SM group increased more significantly.4 Dahuang zhechong pill affects on blood rheology of blood stasis ratsThe results showed that the model group compared with the control group, blood rheology in whole blood viscosity low shear(Low Cut), cut(Cut), high-cut(High Cut), red blood cell deformability index(Erythrocyte deformation index EDI), erythrocyte aggregation index(Erythrocyte aggregation index EAI), erythrocyte rigidity index(Erythrocyte rigidity index ERI) and erythrocyte electrophoresis index(Erythrocyte electrophoresis index EEI) has an increasing trend but no significant difference(P>0.05); Dahuang zhechong pill low dose group compared with the model group, the above indicators are elevated but not statistically significant(P>0.05); Dahuang zhechong pill high-cut, red blood cell deformability index and erythrocyte rigidity index had a decreasing trend, cut, erythrocyte aggregation index and erythrocyte electrophoresis index has tended to increase, there was no statistical learn the difference(P>0.05); Dahuang zhechong pill low dose group compared with the high-dose group and high dose groups in the index of blood rheology reduced even more significantly, there is a trend better than low-dose group, but no significant difference(P>0.05); Dahuang zhechong pill low and high dose group and the control group compared to the above indicators are rising trend, but no significant difference(P>0.05) was statistically; Dahuang zhechong pill low and high dose group compared with the SM group, the above indicators were increasing trend, but no significant difference(P>0.05) statistically, SM group and blank group, no significant difference(P>0.05). Dahuang zhechong pill show high-dose group had lower blood flow change in low-cut, high-cut, red blood cell deformability index, erythrocyte rigidity index trend.Conclusion:1 Dahuang zhechong pill can reduce the P- selectin levels, the degree of inhibition of platelet activation, leukocyte delay to thrombus aggregation, preventing the occurrence of blood stasis.2 Dahuang zhechong pill by increasing levels of t-PA and reducing the level of PAI, the degradation of fibrin(original) and some coagulation factors, coagulation and fibrinolysis system maintain homeostasis, to achieve therapeutic blood stasis purposes.3 Dahuang zhechong pill 6-Keto PGF1α by elevated levels of inhibition of platelet aggregation, regulate 6-Keto PGF1α / TXB2 balance, preventing the generation of blood stasis.4 Dahuang zhechong pill high dose group had lower blood flow change in the index of low-cut, high-cut, red blood cell deformability index, erythrocyte rigidity index trend, preventing red blood cell aggregation, reduces blood viscosity, thus preventing the formation of blood stasis. |
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