| It has been demonstrated by many clinical studies that acupuncture Neiguan can improve heart function.The previous animal experimental research has indicated that electro-acupuncture can ameliorate energy metabolism dysfunction of rat ischemia-reperfusion injured myocardium,decrease the production of reactive oxygen species,inhibit calcium overload and increase endogenous protective substances.But the regulation mechanism that different meridians point treat injury of myocardial reperfusion across blood vessels neogenesis has not been found.This experiment tries to give contrast a research that pericardium meridian and urinary bladder meridian adjust to myocardial ischemia injure.[Objective]At basis that neiguan needle implant can surely promote the afferent density to increase,observe the whole mRNA express characteristic of related member function of vascular growth factor in ischemic injury myocard. by functioning gene array technique.Moreover select important factor and investigate the mechanism of regulation of blood vessels neogenesis in ischemia injured pig myocardium by needle implant Neiguan in gene and protein level,Points Geshu were chosen as control in order to observe blood vessels neogenesis variability,explore the relative specificity of correlations between Pericardium Channel of Hand-Jueyin(point Neiguan) and the Heart, Meridians and viscera.[Methods]32 Guizhou miniswine were randomly divided into four groups including pseudo-operation group,model group,needle implant on Neiguan group,needle implant on Geshu group.Every group have eight.The animal model of myocardium ischemia was set up by ligation of coronary artery.After needle implant Neiguan,Geshu respectively,changes of ST segmen were observed,changes of myocardium morphology observed by optical and electron microscope,detect heart muscle capillary by immunohistochemistry method,discrepancy genes were searched by functioning gene array technique of correlations blood vessels neogenesis,check and analyze mRNA and protein expressions of VEGF and TGF-β3 by Real time PCR and Western blot methods.[Results]①ST segment degraded after ischemia injury.needle implant Neiguan and Geshu can both promote the recoveried ST segment,showed obvious difference from modle group(P＜0.05),but Neiguan group surpassed Geshu group(P＜0.05).②Needle implant Neiguan can accelerate the repairment of histomorphology and ultrastructure of ischemia injured myocardium,increase myocardium capillary density,showed obvious difference from modle group(P＜0.01). Needle implant Geshu had amelioration effects,but Neiguan group surpassed Geshu group(P＜0.05)③the results of Q Series functioning gene array of correlations blood vessels neogenesis suggested:in the way of genes numbers that discrepancy express exceeded two times in myocardium blood vessels neogenesis,changes in Neiguan group exceed to Geshu group,Specific Promoters and Inhibitors, Vascular Endothelial Growth Factors,Fibroblast Growth Factors & Receptors, Cytokines and Chemokines and transcription Factors are common in each group,VEGF,VEGFR,TGFβ,Angiopoietin 1,FGF,FGFR and PDGF are co-expression,furthermore express are above two times.④Needle implant Neiguan can upregulate the expression of VEGF mRNA and protein,showed obvious difference from modle group(P＜0.01).Needle implant Geshu showed a tendency to upregulate the expression of VEGF mRNA,but had not obvious difference from the modle group(p＞0.05),Geshu can also upregulate the expression of VEGF protein,which were not obviously different from the Neiguan group(p＞0.05).⑤Needle implant Neiguan can upregulate the expression of TGF-β3 mRNA and protein,showed obviously difference from modle group(P＜0.01).Needle implant Geshu showed a tendency to upregulate the expression of TGF-β3 mRNA,but had not obvious difference from the modle group(p＞0.05),effect that Neiguan upregulate the expression of TGF-β3 protein,is better to Geshu group(p＜0.05)[Conclusions]①Needle implant Neiguan may protect myocardium tissue through the way of upregulating the mRNA and protein expression of VEGF and TGF-β3 mRNA, destroying balance system of Angiogenesis,enhancing the ability of angiopoiesis,increasing capillary density,promoting establishment of collateral circulation,increasing blood flow of ischemic myocardial.Such protective effect was manifested as improving the electrophysiology and accelerating the repairment of histomorphology and ultrastructure of pig ischemian injured myocardium.②The strong myocardium-protective effect of Needle implant Neiguan probably is one evidence of the relative specificity of the Meridians and viscera..
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