Effect Of Transcranial High-voltage Electrical Burns On Leukocytes Rheological Behavior And Related Mechanism

Objective: As the generally application of electrical in production and life, the incidence rate of electrical burn is increasing annually, and become one of the key heals that endanger people health. High voltage electrical burn, the main component of electrical burn, has high death rate and maim rate for its complex patholo gical variation. So, electrical burn basis and clinical study is being attached importance in medical area.The damage mechanism of electrical burn involves electric-hot, volume-electric conduct, actual electrical damage,micro-circulation obstruction and so on, among microcirculation plays an important part in further damage after high voltage electrical. At the same time, most microcirculation obstruction is reversible. Microcirculation obstruction could be relieved or abate to protect damaged tissues,organs and save lifes after correct treatment.Leucocytes rheological behavior change is an important cause of microcirculation obstruction. Former high-voltage burns microcirculation research focuses on microvascular form and micro hemodynamics, people found that high voltage electrical affect peripheral tissues and visceral microcirculation in different extent, and Leucocytes multiply in micro-veins. but didn't study Leucocytes rheological behavior and related mechanism deeply and systematically yet. This experiment duplicates rats transcranial high voltage electrical burn models through the big, observes mesenteric micro-vessel, studys Leucocytes rheological behavior in micro-veins when the animals is live, and detects the change of P-selectin,E-selectin,L-selectin,sICAM-1 and CD11b/CD18. With Ulinastatin and PTX,this study researchs the affect and related mechanism of Leucocytes rheological behavior in microcirculation obstruction caused from high voltage electrical burn, and applys experiment evidences for the treatment to microcirculation obstruction caused from high voltage electrical burn.Methods: The study consist of three steps, the first to study rheology characters of leukocytes after high-voltage electricity burns (HEB), 45 SD rats were randomly divided into three groups, consisting of control one group, electricical burns one group and treatment one group, 15 for each. The second step to study the effect of every selectin on rheology characters of leukocytes after HEB, 180 SD rats were randomly divided into three groups, consisting of control two group, electricical burns two group and treatment two group, 60 for each, every group was falled into six phases to study, 10 rats for each phase. The third step to study the effect of CD11b/CD18 and sICAM-1 on rheology characters of leukocytes after HEB, 144 SD rats were randomly divided into three groups, consisting of control three group, electricical burns three group and treatment three group, 48 for each, every group was falled into six phases to study, 8 rats for each phase. Used the system made by TC-30-20KV A type voltage regulator and YDJ-10KV A type transformer to reproduce the 2KV high-voltage electricity burns (HEB) animal model in electricical burns groups and treatment groups. Treatment group 1 and group 3 using Ulinastatin at electrical burn immediate time, the treatment group 2 using Pentoxifylline at electrical burn immediate time, the control group no electricity and medicine. At 15mins before HEB and 5mins,1,2,4,8h after HEB to observe. With WX-9 microscope and its image analytical system to observe leucocyte rheological change in mesentery veinule of anterior three groups. Observing indexes: rolling leucocyte count (a/min), rolling leucocyte speed (m/s), adhesion leukocytes, leucocyte-endothelial contact time (TLECT); by enzyme-linked immunosorbent method (ELISA)to measure selectin-P,selectin-E,selectin-L of center three groups , used the LISCA Laser Doppler Hemoperfusion image instrument(LDPI) to observe skin perfusion and used WX-9 microscope to to observe adhesion leukocytes. Used flow cytometric to detect CD11 / CD expression in neutrophils of aboral three groups, by ELISA to measure sICAM - 1 content, used the LISCA Laser Doppler Hemoperfusion image instrument(LDPI) to observe skin perfusion and used WX-9 microscope to to observe adhesion leukocytes. SPSS statistics software were used in the statistic analysis of the data (P < 0.05 level for examination) .Results:1 The comparison of rolling leucocyte count in anterior three groups . The rolling leucocyte count of each phase in control group 1 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the rolling leucocyte count in the electrical burn group 1 were significantly more than before the burn 15min, at 5min after HEB, the rolling leucocyte count was 51.40±3.20, have a trend of increase gradually 1h later after HEB. The treatment group 1 and the electrical burn group 1 have same trend, at 5min after HEB, the rolling leucocyte count in treatment group 1 was 24.60±1.88.The rolling leucocyte count of three groups at 15min before burn intercomparison(P﹥0.05). The rolling leucocyte count of electrical burn group 1 and treatment group 1 in each phase were more than the control group 1(P﹤0.05). The treatment group 1 each phase are less than the electrical burn group 1 (P﹤0.05).2 The comparison of rolling leucocyte speed in anterior three groups. The rolling leucocyte speed of each phase in control group 1 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the rolling leucocyte speed in the electrical burn group 1 after HEB were slower significantly than before the burn 15min, at 5min after HEB, the rolling leucocyte speed was (90.29±8.72)μm/ s, have a trend of increase gradually 1h later after HEB. The treatment group 1 and the electrical burn group 1 have same trend, at 5min after HEB, the rolling leucocyte speed in treatment group 1 was (175.22±13.42)μm/ s.The rolling leucocyte speed of three groups at 15min before burn intercomparison (P﹥0.05). The rolling leucocyte speed of electrical burn group 1 and treatment group 1 in each phase were slower than the control group 1(P﹤0.05). The treatment group 1 each phase were faster than the electrical burn group 1 (P﹤0.05).3 The comparison of adhesion leukocytes in anterior three groups.The adhesion leukocytes of each phase in control group 1 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the adhesion leukocytes in the electrical burn group 1 after HEB were significantly more than before the burn 15min, at 5min after HEB, the adhesion leukocytes was 23.13±3.34, have a trend of increase gradually 1h later after HEB. The treatment group 1 and the electrical burn group 1 have same trend, at 5min after HEB, the adhesion leukocytes in treatment group 1 was 5.87±1.60.The adhesion leukocytes of three groups at 15min before burn intercomparison (P﹥0.05). The adhesion leukocytes of electrical burn group 1 and treatment group 1 in each phase were more than the control group 1(P﹤0.05). The treatment group 1 each phase were less than the electrical burn group 1 (P﹤0.05).4 The comparison of leucocyte-endothelial contact time (TLECT) in anterior three groups.The TLECT of each phase in control group 1 after bogus HEB compared with 15min before the burn , no significant difference(P﹥0.05). But the TLECT in the electrical burn group 1 were significantly more than before the burn 15min, at 5min after HEB, the TLECT was (14.45±1.99)s/ min, have a trend of increase gradually 1h later after HEB. the treatment group 1 and the electrical burn group 1 have same trend. at 5min after HEB, the TLECT in treatment group 1 was(3. 66±0. 96)s/ min.The TLECT of three groups at 15min before burn intercomparison (P﹥0.05). The TLECT of electrical burn group 1 and treatment group 1 in each phase were more than the control group 1(P﹤0.05). The treatment group 1 each phase were less than the electrical burn group 1 (P﹤0.05).5 The comparison of selectin-P in center three groups.The selectin-P of each phase in control group 2 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the selectin-P in the electrical burn group 2 after HEB were significantly higher than before the burn 15min, have a trend of increase gradually. The treatment group 2 and the electrical burn group 2 have same trend.The selectin-P of three groups at 15min before burn intercomparison (P﹥0.05). The selectin-P of electrical burn group 2 and treatment group 2 in each phase were higher than the control group 2(P﹤0.05). The treatment group 2 each phase were lower than the electrical burn group 2 (P﹤0.05).6 The comparison of selectin-E in center three groups.The selectin- E of each phase in control group 2 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). The selectin- E in electrical burn group 2 at 5min after HEB compared with 15min before the burn , no significant difference (P﹥0.05). But 1h later after HEB, have increasing trend and higher than before the burn. The treatment group 2 and the electrical burn group 2 have same trend.The selectin- E of three groups at 15min before burn and 5 min after HEB intercomparison (P﹥0.05). The selectin- E of electrical burn group 2 and treatment group 2 in each phase(1h later ) were higher than the control group 2(P﹤0.05). The treatment group 2 each phase(1h later ) were lower than the electrical burn group 2 (P﹤0.05).7 The comparison of selectin-L in center three groups .The selectin-L of each phase in control group 2 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the selectin-L in the electrical burn group 2 after HEB were significantly higher than before the burn 15min, have a trend of increase gradually. The treatment group 2 and the electrical burn group 2 have same trend.The selectin-L of three groups at 15min before burn intercomparison (P﹥0.05). The selectin-L of electrical burn group 2 and treatment group 2 in each phase were higher than the control group 2(P﹤0.05). The treatment group 2 each phase were lower than the electrical burn group 2 (P﹤0.05).8 The comparison of adhesion leukocytes in center three group. The adhesion leukocytes of each phase in control group 2 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the adhesion leukocytes in the electrical burn group 2 after HEB were significantly more than before the burn 15min, most at 5min after HEB, the adhesion leukocytes have a trend of increase gradually 1h later after HEB. The adhesion leukocytes in the treatment group 2 after HEB were significantly more than before the burn 15min, and have a trend of increase gradually 5min later after HEB.The adhesion leukocytes of three groups at 15min before burn intercomparison (P﹥0.05). The adhesion leukocytes of electrical burn group 2 and treatment group 2 in each phase were more than the control group 2(P﹤0.05). The treatment group 2 each phase were less than the electrical burn group 2 (P﹤0.05).9 The comparison of chest wall skin perfusion in center three group.The skin perfusion of each phase in control group 2 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the skin perfusion in the electrical burn group 2 after HEB were significantly less than before the burn 15min, least at 1h after HEB. The skin perfusion in the treatment group 2 after HEB were significantly less than before 15min.The skin perfusion of three groups at 15min before burn intercomparison (P﹥0.05). The skin perfusion of electrical burn group 2 and treatment group 2 in each phase were lower than the control group 2(P﹤0.05). The treatment group 2 each phase were more than the electrical burn group 2 (P﹤0.05) .10 The comparison of sICAM-1 in aboral three groups.The sICAM-1 of each phase in control group 3 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the sICAM-1 in the electrical burn group 3 after HEB were significantly higher than before the burn 15min, most at 5min after HEB, the sICAM-1 have a trend of decrease gradually 1h later after HEB. The sICAM-1 in the treatment group 3 after HEB were significantly hegher than before the burn 15min.The sICAM-1 of three groups at 15min before burn intercomparison (P﹥0.05). The sICAM-1 of electrical burn group 3 and treatment group 3 in each phase were hegher than the control group 3(P﹤0.05). The treatment group 3 each phase were less than the electrical burn group 3 (P﹤0.05). 11 The comparison of CD11b/CD18 in aboral three groups.The CD11b/CD18 of each phase in control group 3 after bogus HEB compared with 15min before the burn , no significant difference (P﹥0.05). But the CD11b/CD18 in the electrical burn group 3 after HEB were significantly higher than before the burn 15min, most at 5min after HEB, the CD11b/CD18 have a trend of decrease gradually later. The CD11b/CD18 in the treatment group 3 after HEB were significantly hegher than before the burn 15min.The CD11b/CD18 of three groups at 15min before burn intercomparison (P﹥0.05). The CD11b/CD18 of electrical burn group 3 and treatment group 3 in each phase were hegher than the control group 3(P﹤0.05). The treatment group 3 each phase were less than the electrical burn group 3 (P﹤0.05).12 The adhesion leukocytes in aboral three groups and center three groups have same trend.13 The skin perfusion in aboral three groups and center three groups have same trend.Conclusion:1 High-voltage electrical burns can cause leucocyte rheological abnormity change : count increase, rolling speed decrease, adhesion leukocytes increase, TLECT value extend.2 Ulinastatin can reduce abnormity leucocyte rheological that were caused by high-voltage electrical.3 High-voltage electrical burns can increased selectin-P, and selectin-E, and selectin-L and adhesion leukocytes in mesentery venuel, decreased chest wall skin perfusion.4 Pentoxifylline can reduce selectin-P, and selectin-E, and selectin-L ,decrease adhesion leukocytes in mesentery venuel , increase chest wall skin perfusion.5 High-voltage electrical burns can increase sICAM-1, and CD11b/CD18, and adhesion leukocytes and decrease chest wall skin perfusion.6 Ulinastatin can decrease sICAM-1, and CD11b/CD18, and adhesion leukocytes and increase chest wall skin perfusion.7 High-voltage electrical burns can cause leucocyte rheological abnormity change, selectin-P, and selectin-E, and selectin-L, and sICAM-1, and CD11b/CD18 et al play an important role in leukocyte adhesion. Ulinastatin and Pentoxifylline can reduce leucocyte rheological abnormity change.