The Dynamic Change Of GPⅠa/Ⅱa, GPⅣ In The Serum Of Rats Which Were High—voltage Electrical Burn And The Intervention Effection Of Pentoxifylline(PTX) And Ulinastatin(UTI)

Object:As a green and high-efficiency energy,electrical energy play an irreplaceable role in the production and living.However, we should not ignore electrical damage, when electrical energy bring convenience.Especially high-voltage electrical injure has many of the features such as the pathological mechanisms of complex, widespread impact to body, more complications and so on.So the treatment of the high-voltage electrical injure is a troublesome to clinician all the time.Through designing this test,we can offer some references data to clinical work.And we can contribute our achievement to recovery of patient of high-voltage electrical injure.The pre-experimental has proved that the high-voltage electrical injure lead not only platelet Glucoprotein Ⅰb/Ⅸ/Ⅳ(CD42) reduction and GlucoproteinⅡb/Ⅲa(CD41/CD61) rise,but activation of platelet,thrombogenesis,blood stream slow and microcirculation disturbance;Pentoxifylline can reduce the degree of platelet activation and improve microcirchlation disturbance.Through model of rats of high-voltage electrical injure research the effect of high-voltage electrical injure to GPⅠa/Ⅱa and GPⅣ,the effect of pentoxifylline and ulinastatin in this experiment.Methods:1 Group:According to the way of randomized block design, 240 healthy adult male SD-rats(taken from Experimental Animal Center of Hebei Medical University, 300-350 g weight, the certificate number is 1411023) were divided into five groups randomly using random number table,includ blank control group,high-voltage electrical burn group, were treatment group 1(ulinastatin group),treatment group 2( pentoxifylline group),There were 60 rats in the each group.These groups were divided six different time phases,which included 10 rats.Includ ahead(T0), immediately(with in15min)(T1), 1h(T2), 2h(T3), 4h(T4), 8h(T5).Every rat numbered and fed dividually.2 Preparation:According to experimental requirement dispensed the drug.Number and weigh the rats; record data.3 Duplication of high-voltage electric burned model:We needed to connect experimental facilities properly. Rats were anesthetized with chloral hydrate(0.3ml/100g) by intraperitoneal injection.After anestheticed, rats were laid on the electric shock experiments stage. Electrode slice(1cm×1cm) was connected to left upper limb and right lower extremity severally.Left upper limb was extrance. Right lower limb is exit.Then we needed to connect the power source and closed the loop.Finally the rats group was shocked by the 2000 volts electric current for 3 seconds.According to the text rule,blank control group connects electrode slice,but there wasn’t electric current, The rats were injected normal saline 5ml/kg.high-voltage electrical burn group gave were given electric current,but no the dung. UTI(2×104u /kg)was gaven the treatment group1 and 1%PTX(5ml/kg) were given the treatment group2.According to time phase, the above groups were collected blood and made serue.4 Collection and disposition:In different time phases,we fixed the rats on the operating table,then we open pectoral cavity and collected blood about 3ml from each rat’heart(3ml pink coagulation tube) tube.If rats of high-voltage electrical burn waked,we need to inject chloral hydrate(0.3ml/100g) in the abdomial cavity.Usually each rats can collected 6ml blood.After settlement 30 minutes,plasma would form.the tube would be centrifuged with 3000r/min about 10 minute.Then we would collect plasma into 2ml Eppendorf tuber and preserve in ultra low temperature freezer.5 Index test:Applying ELISA double antibody method, according to six different time phases,we detected OD level of GPⅠa/Ⅱa, GP of rats Then Ⅳwe convert OD to concentration according to specification.6 Analysis of Statistics:Applying SPSS 13.0 statistical software,we analysed the data.The experiment data were expressed with the means 士SD.we used two factors factorial design and multiple comparisons with LSD of t test method. P<0.05 was selected as significant standard.Result: 1 the change of GPⅠa/Ⅱa in the blood stream 1.1 serum content of GPⅠa/Ⅱa in high-voltage electrical burn group is generally higher than it in the blank control group(P<0.01),and along with the time change,the content of GPⅠa/Ⅱa express a significant statistical different after electrical injury(P<0.01). The change trend is increased firstly and then decreased.The serum content go to the summit at 2 hours after injury,afterward drops.The GPⅠa/Ⅱa of serum content of high-voltage electrical burn group higher then the blank control group at 8 hours after injury(P<0.05). 1.2 serum content of GPⅠa/Ⅱa in treatment group1(UTI)is generally lower than it in the high-voltage electrical burn group(P<0.01).And GPⅠa/Ⅱa of serum content increase gradually then decrease.It go to the summit at 2 hours after injury.The content of UTI group lower then the high-voltage electrical burn group at 15minute-8 hours after injury and express a significant statistical different(P<0.05). 1.3 serum content of GPⅠa/Ⅱa in treatment group2(PTX)is generally lower than it in the high-voltage electrical burn group(P<0.01).The contend of PTX have complex change after electrical injury.Serum content of GPⅠa/Ⅱa in treatment group2 lower than the high-voltage electrical burn group at 15minute-8hours after injury and have significant change. 2 the change of GP in the blood sⅣ tream 2.1 serum content of GPⅣa in high-voltage electrical burn group is generally higher than it in the blank control group(P<0.01),and along with the time change,the content of GP Ⅳexpress a significant statistical different after electrical injury(P<0.01). The change trend is increased firstly and then decreased.The plasma content go to the summit at 2hours after injury,afterward drops.The GPⅣof plasma content of high-voltage electrical burn group higher then the blank control group at 8 hours after injury(P<0.01). 2.2 serum content of GPⅣ in treatment group1(UTI)is generally lower than it in the high-voltage electrical burn group(P < 0.01).And GPⅣof serum content increase gradually then decrease.it go to the summit at 2 hours after injury.The content of UTI group lower then the high-voltage electrical burn group in the at 15minute-8hours after injury and express a significant statistical different(P<0.01). 2.3 serum content of GPⅣ in treatment group2(PTX)is generally lower than it in the high-voltage electrical burn group(P<0.01).The change of contend of PTX is complex after electrical injury.The serum content of GPⅣin PTX lower then the electric injury group at 15minute-2hours.There is a significant statistical different(P<0.01).And then between them have no a significant statistical different(P>0.05) at 4 hours after injury.There is a significant statistical different at 8 hours after injury.It is express that treatment group higher then electrical group.Conclusions:1 After electrical injury,the serum content of GPⅠa/Ⅱa and GPⅣ have increase in various degree.It prove that high-voltage electrical burn can lead to platelet activation and thrombogenesis.So body will enter into hypercoagulable state.2 The content of GPⅠa/Ⅱa and GP lower then the electrical injuryⅣ in the treatment group 1( UTI).So it prove that UTI help lower serum content in the GPⅠa/Ⅱa and GPⅣ, improve blood high condensation state and prevent the thrombosis.The content of GPⅠa/Ⅱa and GP lower then the electrical Ⅳinjury in the treatment group 2(PTX).So it prove that PTX help lower serum content in the GPⅠa/Ⅱa and GPⅣ, improve blood high condensation state and prevent the thrombosis.