The Change Of FIB, GM-CSF In The Plasma Which Were Received High-voltage Electrical Burn And The Intervention Effect Of Ulinastatin And Methylprednisolone

Objective: High voltage electric burn which is different from the generalthermal burns can cause a complex series of pathological changes.，resulting inthe micro microcirculation microcirculation disorders can lead to rheologicalchanges which platelets and the number of anomalies is one of the key factorsleading to the body’s microcirculation. After the burn damage or rupture ofblood vessels, platelets are activated by stimuli from the stationary phase tothe phase function, further affecting platelet production.Thrombopoietin(thromboietin,TPO) and megakaryocyte colony stimulating factor(megakaryocyte colony stimulating factor, MK-CSF) is a positive regulator ofplatelet production.When the body thrombocytopenia, TPO, and MK-CSFregulate proliferation of megakaryocyte progenitor cells to producemegakaryocytes, MK-CSF stimulation of megakaryocyte maturation andpromote megakaryocyte cytoplasm and nucleus from the split form andrelease into the blood platelets. IL-3and IL-6plays in the formation ofplatelets positive regulatory role, IL-3stimulates megakaryocyte proliferation,IL-6may accelerate the maturation of megakaryocytes and platelets acceleratethe generation of both synergistic.Granulocyte-macrophage colony-stimulating factor (granulocyte-macrophage colony stimulating factor,GM-CSF) and erythropoietin (Erythropoietin, EPO) on megakaryocytes toproduce platelets also have non-specific positive regulatory role.Which IL-3,granulocyte-macrophage colony-stimulating factor (granulocyte-macrophagecolony stimulating factor, GM-CSF) is a positive regulator of plateletproduction is an important element, to reflect changes in platelet count andcoagulation processes. Therefore, we designed this experiment to study thehigh-voltage burns early rat serum IL-3, GM-CSF changes, Through the intervention of ulinastatin, and MP we discuss the expression on themechanism of thrombopoietinand rheologicalchange after studying burn，andexplore the feasibility of early treatment.Methods:1Animal groups:240healthy adult male SD rats(Hebei MedicalUniversity Experimental Animal Center of certificate No.1303144)weredivided into three groups according to the random number table, fakehigh-voltage burns group (control group), high-voltage burn group (electricalinjury group), high-voltage burns UTI MP treatment group (treatmentgroup).Each group was composed of60SD rats.According to the randomnumber table each group was divided into six-phase group,15min beforeelectric shock,5min after electric shock,1h,2h,4h,8h after electric shocksix-phase group.Each phase group was composed of10SD rats.Preparation: The rats were numbered, and weighed. Then shuck off therats’ feather of left upper limbs, light legs and the chest. Experimental drugsprepared in accordance with the desired concentration.Modeling of high voltage electric burn: Connect the transformer andpressure regulator wire. Rats are anesthetized with1%sodium pentobarbitalby means of intraperitoneal injection,40mg/kg administered. After thesuccess of the anesthesia, put rats in special shock experiments on the stage,fixed limbs, then two1cm×1cm electrode plate were fixed in rats’ left upperlimb (current entry), the right lower limb (current export) depilatory area.Switch on the power, adjust to boost pressure regulator of output voltage to2kV, connect the power of output, high-voltage electric gets through rat, shocktime is3s.The control group which offers only even wires and no electricityproduce false electrical injury model. Rats with electric injury within5min,offer intraperitoneal injection of5×104u/kg UTI、250mg/kg MP in treatmentgroup, and the control group and the experiment group are injected of sameamount of saline by means of intraperitoneal injection.4Specimen collection and preservation:Operate the rat model which arereplicated successful, open the chests, exposure heart, collect open-heart blood 6ml, Wherein is placed in a one-time use of vacuum vessels(red),and gentlyinvert several times and stand for30min, put the serums in centrifuge, to3000r/min, centrifuge10min, take clear liquid in red tube to Eppendorf tubeand preserve them in-70℃conditions.5Index test: Serum placed in the refrigerator was taken ELISA doublesandwich antibody method to detect the content of fibronectin.6Analysis of Statistics:Using SPSS13.0statistical software to finishvariance analysis of two factors factorial design, multiple comparisons withLSD of t test method. It has significant statistically meaning if P<0.05.Results: The changes of IL-3content in rats’ plasma1IL-3contents in electrical injury groups are generally higher thanthose in control groups (P<0.01),and IL-3content over time after the injuryshowed significant differences (P<0.01), showing a rising trend, but thechange is more complex.IL-3contents in UTI treatment groups aregenerally lower than those in electrical injury groups (P<0.01); and IL-3contents over time after the treatment showed significant differences (P<0.01),but only in the treatment group, the level of content in5min afterinjury is higher than the value of this group before injury (P<0.01),2h,4h,8h after injury is no significant difference with the group of before injury(P>0.05).2The changes of GM-CSF content in rats’ plasmaGM-CSF contents in electrical injury groups are generally higher thanthose in control groups (P<0.01),and GM-CSF content over time after theinjury showed significant differences(P<0.01),and GM-CSF contents overtime after the treatment showed significant differences (P<0.01).In thetreatment groups after injury, from1h to8h, the level of content in eachphase is higher than the value before injury (P<0.001).Conclusion:1IL-3, GM-CSF of rat plasma in the high-voltage burns early haveincreased to varying degrees. It is proved that there is existing an increaseof platelet generate to abnormalities rheology high condensation state, fibrinolytic system dysfunction and microcirculation after burn.2In high-voltage electric burns UTI、MP treatment group, the contentof IL-3, GM-CSF reduces compared with electrical injury group. It suggeststhat UTI、MP can inhibition of plateletactivation andgeneration, and plateletrheology so as to improve the microcirculation and prevent the formation ofblood clots.