| Background: In the preceding high-voltage electric burned animal exper-iments, we have observed microcirculatory changes of mesenteric, intestinalmucosa, the cerebral pia mater, liver, pancreas, kidney cortex, gastric mucosa,deep fascia by WX-9B poly-position microcirculation instrument, and demons-trated the existence of microcirculation disorder in high-voltage electric burne-d animal modles. We also can found microcirculation disturbance caused bythe high-voltage not only in peripheral tissue, but also in distant tissue andorgans, leading to systemic microcirculation. Qbserved flow phenomenon incapillary, phenomenon of leukocyte rolling and adhesion along the vascular,phenomenon of white small thrombus mobility and erythrocyte aggregation. Itis one of the important changes that leukocyte adhere to vascular endotheliumafter burning. It was mediated by intercellular adhesion molcule-1(ICAM-1)and β2-integrin including lymphocyte founction-associated molecule-1(LFA-1, CD11a/CD18), macrophage surface antigen-1(Mac-1,CD11b/CD18),P150,95(CD11c/CD18) and αDβ2(CD11d/CD18,). In our proceeding animalexperiment, we had demonstrated that soluble intercellular adhesion molecule-1(sICAM-1) and macrophage surface antigen-1(Mac-1,CD11b/CD18) haveincreased in high-voltage electrical burned animal modles, however, correlatedstudy of CD11c/CD18is a relatively infrequent in high-voltage electrical burn(HEB), therefore we design the high-voltage electrical burned experiment.Objective: To observe the rate of positive expression of CD11c/CD18inleukocytes and the effect of pentoxifylline (PTX) and ulinaotatin(UTI), andinvestigate the pathogenesis of microcirculation disturbance in early high-voltage electric injury stage. We may offer theoretical foundation for theclinical therapy of HEB. Methods：1. Group:240healthy grown up S-D rats (taken from ExperimentalAnimal Center of Hebei Medical University, regardless of sex, no pregnant,280-360g weight, and the certificate number is786657) are randomly dividedinto four groups, and60rats in each group. The groups are fake high-voltageelectrical burned group(control group), high-voltage electrical burned experim-ent group (experiment goup), high-voltage electrical burned PTX reatmentgroup (PTX treatment group) and high-voltage electrical burned UTI reatmentgroup (UTI treatment group). There are six different time phases for eachgroup, ahead(T0), immediately(15min)(T1),1h(T2),2h(T3),4h(T4),8h(T5),and10rats are prepared for each time phase.2. Preparetion: Weigh the rats, number them, moult the rats in inner sidesof the left upper limbs, right lower limbs and belly, and put them in24-26℃and35-45%RH laboratory, supply no food but free water for a night.Constitute the experimental drug concentration according to experimentalrequirement.3. Duplication of high-voltage electric burned model: Anesthetic rats areburned by the1000volts high-voltage electric system for3seconds. They areduplicated by full-thickness electrical burn with body tissues that touch withthe electrodes. We immediately inject PTX (50g/kg) to rats of PTX treatmentgroup and UTI (20000u/kg) to rats of UTI treatment group through abdomen.Inject normal sodium to experiment group and control group. The progress ofcontrol group is the same as the electric burn group, but there is not electriccurrent in the body of control group rats.4. Collection and disposition: In different time phases, open pectoralcavity, take suction blood about1.5ml from each rat’ heart to k2EDTA plusblood collection tube, lyse erythrocyte, mark immunofluorescence monoclonalantibody, and finally constitute the leucocyte suspension.5. Detection index: Flow cytometric analysis (FCM) is used to detect therate of positive expression of CD11c/CD18in leukocytes of rats at differenttime phases after HEB. 6. Analysis of Statistics: Statistically analysis all data with SPSS13.0. Alldates should be expressed withx±s. we apply three factors factorial designof variance analysis among groups. It has significant statistically meaning ifp<0.05.Results: Expression of CD11c/CD18levels1. The expression levels of CD11c/CD18did not have obviously changewithin the control group.(P>0.05)2. Compared with the time phase before HEB and the control group, therate of positive expression of CD11c/CD18of the experiment group increasedobviously in early postburn stage (p<0.05). The expression levels of CD11c/CD18reached a peak later in4hours after HEB, and decreased. Within theexperiment group, there were significant changes between adjacent timephases (P <0.05).3. Compared with the time phase before HEB and the control group,therate of positive expression of CD11c/CD18of the PTX treatment groupincrease obviously also in early postburn stage (p<0.05). The rate of positiveexpression of CD11c/CD18increased gradually after HEB in8hours. Withinthe PTX treatment group, there were significant changes between T1and T2,T2and T3, T4andT5(P <0.05), but no obvious change between T3andT4(P>0.05).4. Compared with the time phase before HEB and the control group, therate of positive expression of CD11c/CD18of the UTI treatment group increas-ed obviously in early postburn stage (p<0.05). The expression ofCD11c/CD18reached a peak later in4hours after injury, and decreased.Within the UTI treatment group, there were significant changes between T1and T2, T2and T3, T3and T4(P <0.05), but no obviously change within T4andT5(P>0.05).5. Compared with the experiment group in each time phase, the rate ofpositive expression CD11c/CD18were decreased in the PTX treatment group.There were significant changes at T3, T4and T5time phases (P <0.05), but noobvious changes at T1and T2time phases (P>0.05). 6. Compared with the experiment group in each time phase, the rate ofpositive expression CD11c/CD18was decreased in the UTI treatment group.There were significant changes at T2, T3, T4and T5time phases (P <0.05),but no obviously change at T1time phases (P>0.05).7. Comparing the UTI treatment group with the PTX treatment group ineach time phase, the rate of positive expression CD11c/CD18did not haveobvious changes at T1, T2, T3and T4time phases (P>0.05), but have obviouschange at T5time phases (P <0.05).Conclusions:1. High-voltage electrical burn (HEB) can lead to upregulation of CD11c/CD18in leukocytes of rats, and increase the leukocytes adhesion. It may playan important role in microcirculatory disorders.2. PTX and UTI can downregulate the expression of CD11c/CD18inleukocytes, inhibit leukocytes adhesion, and improve microcirculationperfusion. However effects of PTX and UTI do not have obvious difference. |
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