| Gastric ulcer is a common clinical digestive disease, in traditional Chinese medicine,it is included in "stomachache", "fullness" and similar areas. The clinical manifestations are mostly boring epigastric pain associated with swelling of the fullness, acid regurgitation, nausea, vomiting, embolism, and easy bleeding, perforation, obstruction, and cancer, endangering human health. Short-term cure for the disease is no longer a problem, but the prognosis for recurrence remains a medical problem. Chinese medicine to disappearing carbuncle and growing muscle method used in clinical treatment of peptic ulcer therapy is effective with low recurrence rate.Purpose:This topic is for the purpose of seeking the curative mechanism of the traditional Chinese medicine to disappearing carbuncle and growing muscle method treated in acetic acid of peptic ulcer's,which is innovative applications standing by"toxic heat"cause and using"eliminate ,hold ,fill"method of treatment for surgical diseases.Through we study and contract in aspects of histopathology, molecular biology, serology and so a comparative study,based on series of experiments of predecessor`s works, to explore the disappearing carbuncle and growing muscle method mechanism of the treatment of gastric ulcer and recurrence prevention in order to prove gastric ulcer"toxic heat"as the cause .Materials and methods:1. Experimental animal group therapy: SPF grade SD 70 rats , male, body weight (200±10) g. The rats used were randomly divided into 7 groups, namely normal control group (normal group), sham operation group (sham group), model control group (model group), xiao yong kui de kang group, (kui de kang group ), the Chinese medicine decoction group (decoction group), ulcer capsule group (capsule group), omeprazole group, 10 in each group.2.Production of acetic acid induced gastric ulcer model: improve the traditional burning method with acetic acid, to produce rat gastric ulcer model. 60kg calculated according to the weight of ordinary people, people with 200g of rat body weight conversion ratio of 6.25:1, calculate the corresponding concentration of each drug: Disappearing carbuncle and growing muscle decoction 1.28g/kg, Kui de kang granule 0.2 g/kg, ulcer capsule 0.018 g/kg, Omeprazole tablete 0.2 mg/kg.3.Experimental Method: The groups of rats were fed with pellet feed at full size, free feeding, free drinking water, after two days of experimental feeding adaptation.After 24-hour fasting, modeling was started, after 2 days, starts to gavage, two times every day, altogether 12 days. The normal group, normal raising, does not carry on gavage; The sham-operation group, the model group, gavage distilled water 2ml two times every day; The decoction group, gavage 1.28g/kg decoction solution 2ml two times every day; Kui de Kang group, gavage 0.2g/kg density to Kui de Kang solution 2ml two times every day; The capsule group, gavage 0.018g/kg every day the density ulcer capsule solution 2ml two times every day; Omeprazole group, gavage 1.28g/kg density Omeprazole solution 2ml in morning, gavage distilled water 2ml in everning.Gavage for 12 days, after a night of fasting subjects. Rats in each group with 10% chloral hydrate anesthesia, laparotomy, the stomach found in the pylorus, cardia cut at both ends to remove the stomach, making biopsy, frozen for inspection. Took blood form abdominal main blood vessel, centrifuged to obtain supernatant, froze to reserve. The rats could be exposed to water during the whole process of experiment.4. Histological evaluation of gastric ulcer: the ulcer lesion or ulcer scar and surrounding tissue was fixed with the conventional formaldehyde, paraffin-embedded and serial sections, and coloured with HE, using optical microscopy observe pathological changing , mucosal thickness was also measured.5. Serum superoxide dismutase (SOD) detection: enzyme immunoassay kit purchased,use of ELISA method.6.Serum prostacyclin (PGI2), thromboxane (TXA2): enzyme immunoassay kit purchased,use of ELISA method .7. Serum transforming growth factor (TGF) detection: enzyme immunoassay kit purchased,use ELISA method to detect TGF level. Use RT-PCR to detect the expression of growth factors.8.The use of drugs: Chinese medicingto disappearing carbuncle and growing muscle method Decoction, Xiao yong kui de kang granules, capsules ulcer, omeprazole magnesium enteric-coated tablets; 100% glacial acetic acid, 10% solution of chloral hydrate, 10% formaldehyde solution, aprotinin; SOD kit;PGI2, TXA2 ELIASA enzyme immunoassay kit; TGF ELIASA enzyme immunoassay kit; DEPC; 100% ethanol.9. Experimental apparatus: TDL-5A Bench Centrifuge, LeicaRM2135 slicer, Olympus optical microscope, SN-682-type radioimmunoassay r counter, DU-600 nucleic acid analyzer, Electric thermostat temperature box, constant temperature oscillator, 721B spectrophotometer, INFINITE M200 microplate reader; vernier caliper;.Results:1, The results showed pathological damnification of rats is moste severe of the modern group rats,which mucosa is discontinuous ,the deeper ulcer reaches the muscle and chorion ,necrotic tissue on the top of it ;the most gentle of the decoction group rats,regenerated mucosa and acestoma tissue and scar tissue observed .2, The thickness of the regenerated mucosa of rats from thick to thin is from the normal group, decoction group, sham operation group, Kui de kang group, Omeprazole Group, capsule group to model group. Among them, the model group was significantly differently from all the other groups (P <0.05); Kui de kang group was also very different from model group, capsule group and Omeprazole Group (P<0.05); Kui de kang group was also different from model group and capsule group (P<0.05); while similar to Omeprazole group (P> 0.05).3, The SOD content of the rats descent from decoction group through Kui de kang group, Omeprazole Group, capsule group to model group. Among them, the model group was significantly different from all the other groups. (P <0.05); decoction group was also very different from model group, capsule group and Omeprazole Group. (P<0.05); Kui de kang group was not much different from capsule group and Omeprazole Group (P>0.05).4,The PGI2 content was seen lowest in the model group,PGI2 content from high to low are: decoction group, Omeprazole Group, Kui de kang group, sham operation group, capsule group, the normal group and model group. Among them, the model group was significantly different from all the other groups(P <0.05); in decoction group , PGI2 were higher than the normal group, sham operation group, compared with a very significant difference (P <0.01); decoction group was very different from model group, Kui de kang group, capsule group and the Omeprazole group (P <0.05); Kui de kang group and model group were significant different (P <0.05);while not much different compared with the capsule group and Omeprazole group (P> 0.05).5, The highest content of TXA2 was found in the model group, TXA2 content from low to high are seen in normal control group, sham operation group, decoction group, Kui de kang group, capsule group, Omeprazole group and the model group. Among them, the model group and other groups were significantly different (P <0.05); decoction group was significantly different from the model group, the capsule group and the Omeprazole group (P <0.05); but not much different from Kui de kang group (P>0.05); Kui de kang group was significantly different from model group (P <0.05), while compared with the capsule group and the Omeprazole group, it was not much different (P> 0.05).6, The lowest ratio PGI2/TXA2 was seen in the model group, the ratio of PGI2/TXA2 of group of rats from high to low was from normal control group,through decoction group, sham operation group,Kui de kang group, capsule group, Omeprazole group to model group. Among them, the model group and other groups was significant different (P <0.05); decoction group was significantly differently from model group, Kui de kang group, the capsule group, and the Omeprazole group(P <0.05); Kui de kang group compared with the capsule group was significantly different (P <0.05), while not much different compared with the Omeprazole group(P> 0.05).7, TGF content was found lowest in the model group, TGF in groups of rats descent from decoction group, Kui de kang group, Omeprazole Group, the normal group, sham operation group, capsule group and the model group. Among them, the model group and other groups was significantly different (P<0.05); decoction group was also significantly different from the model group, the capsule group and the Omeprazole group (P <0.05); while not much different from the Kui de kang group (P>0.05); the Kui de kang group was significantly different from the model group (P <0.05), but not much different compared to the capsule group, and the Omeprazole group (P> 0.05).Conclusion: 1, Chinese medicine to disappearing carbuncle and growing muscle method can hasten absorb of necrotic tissue ,increase the thickness of regeneration of mucosal.It will promote gastric epithelial cell proliferation, resulting in the rapid formation of granulation tissue, and promote the quanlity of ulcer healing.2, Chinese medicine to disappearing carbuncle and growing muscle method will increase the content of SOD serum levels, lessen free radicals injured.therefore, we believe that Chinese medicine to disappearing carbuncle and growing muscle method has strong mucosal protection and regeneration promotion.3,Chinese medicine to disappearing carbuncle and growing muscle method will reduce the content of TXA2 serum levels, therefore increasing serum levels of PGI2, relieve inflammatory medium release , reduce inflammation, reduce local vascular spasm, and enhance the defensive function of gastric mucosa in order to promote quanlity of ulcer healing and prevent ulcer recurrence.4, Chinese medicine to disappearing carbuncle and growing muscle method can effectively enhance TGF activity to near normal levels, maintain mucosal integrity and defensive function, and adjust the gastrointestinal mucosal blood flow, so as to achieve the purpose of promoting ulcer healing and prevent ulcer recurrence.5, We believe that Chinese medicine to disappearing carbuncle and growing muscle method can promote the quanlity of ulcer healing,is an exact and effective method to cure experimental gastric ulcer, and is expected to achieve a certain breakthrough in preventing ulcer recurrence.6, Gastric ulcer disease is caused by a "toxic heat", the Chinese medicine to disappearing carbuncle and growing muscle method ,based on the surgical treatment of carbuncle ulcers using the "eliminate, hold, and fill " method will add practical applicated value to a peptic ulcer.
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