The Research On The Immune System Reaction Of Rats And Relevant Mechanisms To Hypoxic Training Of Different Models

Hypoxic training is developing these years. It has been proved that immoderate exercise leads to inhibition of immune system in normal oxygen environment and hypoxic environments also cause immunodeficiency. The level of stress increases seriously during hypoxic training because body undertakes the stimulus of both exercise and hypoxia. But the study about the mechanism of hypoxic training is not enough, especially about immune function and its mechanism. The researches mainly focus on the aerobic training and there is a divergence of the results now. The changes of immune function after hypoxic training of high intensity have not been reported because there is not a moderate model of high intensity training.Objective: 1 set up a model of high intensity training; 2 inquire into the effects of moderate and high intensity HiLo and hypoxic training on the raising of aerobic and anaerobic ability; 3 find out the law of immune system reaction to moderate and high intensity HiLo and hypoxic training, provide scientific basis for immune function supervising during hypoxic training; 4 study the possible mechanism of immune function changes after hypoxic training of different model; 5 explore the influence of oxygen environment under different training intensity, provide the theory foundation for making the hypoxic training plan rationally.Method: 225 rats were randomly divided into 24 combinations according to the oxygen environment [normal o₂ , living high and training low (HiLo) , living low and training low (LL) ]and the training intensity [no training , moderate intensity training (MT) and high intensity training (HT) ]and the load of exercise before drawing samples (no load and regular exercise ).The period of training was 5 wks (MT) and 6 wks (HT) respectively. We drew the blood to measure the white blood cells (WBC) and their classification, interleukin (IL)-2, IL-4, stress hormone and super oxidative level, and drew the spleen and separate lymphocyte cells and nature killer cells (NK) to measure the function of lymphocyte proliferation and NK activity.Conclusion: We set up animal models of moderate and high intensity hypoxic training of different methods. Our results showed that HiLo and LL could improve not only aerobic ability (the effect of LL was better) but also anaerobic ability (there were no difference between two groups) , but they brout changes of immune system. Compared with the training in normal o₂, training in LL had no significant effect on the immune system in quiet state and the immune system was even economical in regular exercise Stat. But HiLo did harm to the immune system in quiet state, especially the aerobic training. The influence of oxygen environment and training intensity on different function of immune system in quiet state was different. The oxygen environment had independent influence on B lymphocyte proliferation and the training intensity did on WBC, B lymphocyte proliferation and NK activity. Compared with normal 02 and HiLo enviument, LL environment could stimulate B lymphocyte proliferation. Compared with no training and MT, HT could decrease the number of WBC and monocyte; improve the function of B lymphocyte and NK activity.WBC and its classification, NK activity, IL-4 is the effect targets to judge the changes of immune system during hypoxic training of different models. WBC and its classification could be used as the first target because it can be measured easily and is highly sensitive to hypoxic training.There were close relationships among the changes of immune system and the neuro-endocrine, oxidative stress. The increase of cortisol, ACTH, oxidative level and the decrease of synthetic hormone was the important mechanism of the immune system injury of HiLo.