The Research Of The Conduction Resistance Physiological Mechanisms And Effect On Yield Quality In Grafting Flue-cured Tobacco

This paper studied the studied the different genotypes of flue-cured tobaccovarieties (or lines) grafting on flue-cured tobacco growth and development, productionquality traits, black shank disease resistance, drought resistance and easy to bake andphysiological mechanism in pot experiment and field experiments and indoor test with themethod of application of physiological and biochemical and molecular biology method, Themain results were as follows:1, with disease resistance not the root stock grafting disease-resistant scion, blade of PODand CAT activity increased, gsh-px activity; Rhizosphere bacteria increased significantly;Root GST gene expression level, SOD genes, Hsr203j, BG gene expression of cut; Graftingof tobacco black shank disease incidence rate significantly reduced.2, scion grafting drought resistance is poor, strong drought resistance of the stock and scion,compared to natural plants under drought stress conditions, grafting photosynthesis declineis small, can still maintain a certain growth potential, the blade of a significant rise in SODand CAT activity, proline, malondialdehyde content increased, the drought resistance gene,AREB CDPK2gene, gene and ERD10C LEA5gene expression.3, with nicotine and potassium accumulation of high efficiency genotype for stocks and canimprove the scion of nicotine and potassium accumulation efficiency low nicotine andpotassium content of tobacco leaves, use nicotine and potassium accumulation, lowefficiency genotypes for the stock can reduce nicotine and potassium accumulationefficiency of scion nicotine and potassium content of tobacco leaves.4, using fresh tobacco maturity index calculation method, the proposed quantitativevalidation of the different varieties, different maturity and different parts of tobacco maturityindex. Mapped the camera obscura yellow varieties under different conditions, the area, thematurity of tobacco leaf maturity index curve, prove that different types of roasting andbaking resistance, good varieties for stock in baking properties, which can improve poorbaking characteristics of scion of roast and bake resistance.5,8-10true leaf in the stock and scion leave1-2leaf, cleft grafting method is used to cut orgrafting of tobacco seedling grafting efficiency is highest. The field early grafting grewmore natural miao lag10-15days, gradually catch up with, in the late wang zhen long fieldgrowth period are basically the same; Grafting field performance leaf shape, leaf color, leafAngle characteristics such as the same as the scion, and leaf number, stem circumference,the phenotype production between rootstock and scion; Grafting after the appearance of the tobacco leaf quality and scion basic same, identity, oil content, such as length ofperformance under the influence of the scion; Chemical composition and content of neutralaroma substances between stock and scion, present a partial root stock or partial scion isneutral, parts processing exhibiting pro effect; Under the condition of moderate adversity,after grafting to roast tobacco aroma, mixed gas and the concentration of the quality ofaroma, concentration of aroma, smoking index effect was not significant, to stimulus andfinish a certain improvement.