| The first step of this research is that T. amurensis’s ectomycorrhiza, collected in Cold water national forest reserve, Laoshan ecological station of MaoEr mountain and northeast forestry university campus of Heilongjiang Province, were initially classified by the morphological and anatomical features.Then, with fresh and puerile root tips of different types ectomycorrhiza as experiment materials, the homologous species of ectomycorrhizal fungus were identificated by Molecular identification. On this basis, ectomycorrhiza’s community composition were investigated. Then, the author explored and analyzed the relationship between the mycorrhizal infection rates of T. amurensis and the soil factors in Laoshan ecological station of MaoEr mountain. Finally, T. amurensis’s annual seedlings were singly inoculated by 2 ectomycorrhizal fungus which have grew mainly under T. amurensis. The author compared drought resistance of different treatments by continuous drought stress. The results as follow:1. The Ectomycorrhizal resources of T. amurensis were investigated in 3 areas of Heilongjiang province. Combining with structure feature of the external morphology, inside and outside surface structure of mantle, Hartig net and External hyphae et al, the author preliminary divided into 18 Ectomycorrhizal types of T. amurensis. Subsequently, ectomycorrhizal fungus of T. amurensis were identified by ITS-PCR technology, the preliminary identification 17 species of ECMF were identified,2 Inocybe sp.,1 Boletus sp.,1 Tuber sp.,3 Leccinum sp.,4 Lactarius sp.,3 Russula sp.,1 Tomentella sp.,1 Thelephora sp., and an unknown fungi had not been identificated.2. There were 13 mycorrhizal types in cold water national forest reserve, mycorrhizal infection rate was 26.8%; there were 12 mycorrhizal types in Laoshan ecological station of MaoEr mountain, mycorrhizal infection rate was 43.1%; there were 9 mycorrhizal types in Northeast Forestry University campus, mycorrhizal infection rate was 61%. the relative abundance and frequency of Tuber sp.l and Boletus sp.1 were higher in Laoshan and Liangshui. Diversity index of ECM in Liangshui and Laoshan were relatively higher than Northeast Forestry University campus.3. The relationship between ectomycorrhizal colonization and rhizosphere soil fertility of Tilia amurensis Rupr. in Laoshan ecological station of Maoer mountain was analyzed by means of biostatistics methods, Pearson correlation analysis and stepwise regression analysis. The results showed that there were highly significant negative correlations between ectomycorrhizal colonization rates and four parameters which are alkaline phosphatase, alkaline hydrolysis nitrogen, available phosphorus and soil water content; ectomycorrhizal colonization rates and urease showed highly significant positive correlation; ectomycorrhizal colonization rates and available K showed significantly positive correlation. The main soil fertility factors which impact ectomycorrhizal colonization rates of Tilia amurensis Rupr. are alkaline phosphatase and soil water content.4. Annual seedlings of T. amurensis were singly inoculated by Agaricus silvaticus Schaeff.: Fr.and Leccinum scabrum (Bull, ex Fr.) Gray., the results showed that mycorrhizal colonization of seedlings inoculated by Agaricus silvaticus Schaeff.:Fr. and Leccinum scabrum (Bull, ex Fr.) Gray, respectively were 17.8% and 35.2%. In the continuous drought stress, the biomass of seedling inoculated ECMF were inordinately higher than the compared seedling, the biomass of seedlings inoculated by Leccinum scabrum (Bull, ex Fr.) Gray, were significantly higher than other treatment. And the stress physiological indexes of seedlings inoculated by Leccinum scabrum (Bull, ex Fr.) Gray. were obviously higher than other treatment. Rhizosphere soil enzyme activity of seedlings inoculated ECMF were relatively stabler than the compared seedling. |
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