| As an physics agricultural technology, crop seeds treatment by means of HVEF has the characteristics of the easy to operate, low consume efficiently, low investment and no pollu--tion. The suitable treatment can enhance the seed vigor efficiently. I experiment the seeds germination in the tissue culture laboratory of Yuncheng Agricultural Vocational Technical College, testing of physiological and biochemical indexes of seeds germination in the physiological laboratory of Cotton Research Institute, Shanxi Academy of Agricultural Sciences, testing of field experiment in the lanhua farm of Cotton Research Institute, Shanxi Academy of Agricultural Sciences. In the study, the method of quadratic general rotary unitized design was adopted in the experiment, sorghum seeds (Jinza 122) were processed by high-voltage electronic field generating device. Firstly, the study simplified seven seeds germination indexes into comprehensive index Z by principal component analysis to establish regression model of two factors(electric field and treatment time) and Z, parsed it to obtain optimized areas of HVEF treatment; Secondly, the study analyzed the causes of this change of physiological and biochemical indexes of seeds germination in the optimized areas by completely random experimental design,and studied its mechanisms to obtain the optimal conditional; Thirdly, the study analyzed the physiological and biochemical indexes and photosynthetic characteristics of field sorghum leaf in the optimal conditional by randomized block design to reveal the growth effects of electric field on sorghum seeds during seedling period. The conclusion is as follows:Firstly, testing results of seeds germination in the range electric field strength was 100-800 kV/m, and treatment time was 5-60 min by quadratic general rotary unitized design indicted that the germination indexes had significant difference (P<0.05), compared to the control. Germination rate, germination potential, bud length, root length, germination index, fresh weight, vigor index of all the treatment all is higher than the control group except germination rate of T4、T6 and fresh weight of T5. In order to simplify the procedures, I simplified seven germination indexes into comprehensive index Z to obtain the mathematical model:Y=2.36600+0.64096X,+0.56077X2-2.61613X12-0.76112X22-1.08750X1 X2. The main factor effect of the model analysis shows that the effects of electric field strength were more than that of treatment time on the comprehensive index Z, and explained that the effects of HVEF on sorghum seeds exit a threshold effect, the electric field threshold is the key to cause the biological effect, but treatment time is a very important factor. So we must think about the treatment time when we select the optimal electric field dose; The model indicated that the influence of electric field strength and treatment time on the comprehensive index Z had thresholds effects, the reason was that organisms showed boost, restraint and no response in a different dose. Meanwhile, the interactive influence of electric field strength and treatment time on the germination comprehensive index Z presented that respectively a negative and a positive effect which was significant (P<0.05). After optimizing the model, the optimization scheme which should be met simultaneously that the germination comprehensive index of Jinza 122 were greater than or equal -0.1723 was that electric field strength was 400-600 kV/m, and treatment time was 20-55minSecondly, testing results of physiological biochemical in the seed germination indicated that the treatment could enhance the restorability of cell membrane, protective enzyme activity, a-amylase activity, SP content and mass fraction of endogenous GA; analysis of main effect indicated that it had a significant correlation among various physiology index but CAT, and EC, endogenous ABA and other indexes showed a significantly negative correlation(p< 0.05); the accumulative contribution rate of the first components and the second components accounted for 86.07% which reflected correlation information of all the indexes. The results of principal component analysis showed the effects in sequence is T5, T4, T2, T3, T6, T1, T7, T8, T9,CK.Thirdly, testing results of physiology, biochemistry, photosynthetic characteristic showed that CHI, N, Pro, SP content and SOD, POD activity of treatment were higher than that of controls in seedling stage, and the affecting order was T1,T2,T3. CHI and N content, SOD activity of leaf had significant difference but other indexes between blocks (P<0.05); compared to the control, all physiology and biochemistry indexes had significant difference among treatments(P<0.01). photosynthetic characteristic of leaf in seedling stage showed that Pn, Tr, GS were higher than that of controls in each period, the order was T1,T2,T3,CK; Ci were were all lower than those in normal group in each period. It has shown positive correla--tion between change in value of Pn and that of Tr, GS, opposite to Ci. All those indicated that the increase of Ci could was the main factor of enhancing Pn. |
PDF Full Text Request