Realgar Nanocrystalline Induced Apoptosis In Human Lung Cancer Cell Lines And Its Anti-angiogenic In Vitro

Objective:To study the toxic compound attack "Liushenwan" in the main drugs "realgar" in nanoparticles and cisplatin-induced apoptosis in cultured A549cells and anti-angiogenic mechanism,then attack drug therapies and treatment of lung cancer Research.Methods:Human lung adenocarcinoma A549cells were cultured in vitro, the toxic compound attack "Liushenwan" in the main drug "realgar" nano-technology, with different concentrations of nanometer realgar (25ug/ml,50ug/ml,100ug/ml) and cisplatin (2ug/ml) alone or in combination with, A549cells under the microscope and morphological changes in the state; Annexin V/PI double staining to detect cell apoptosis; MTT assay inhibition of cell proliferation; Immunohistochemistry used to detect Bcl-2, Caspase-3expression; RT-PCR assay Survivin, c-Myc in the mRNA; immune staining integrinβ1and E-cd; immunocytochemistry method β-catenin; flow cytometry detection of b-FGF, MMP-9expression. Nanometer realgar of A549human lung cancer cells inhibited cell proliferation, induce apoptosis, anti-metastasis and the possible role of anti-angiogenesis mechanism for attacking drug therapies based on the treatment of lung cancer provide the basis for theory and laboratory evidence.Results:Different concentrations of nanometer realgar can damage human lung cancer A549cells in the normal form; low concentrations to induce early apoptosis realgar nano-based, high concentrations of nanometer realgar Induced apoptotic based; on A549cells was significantly inhibited, and cisplatin a synergistic effect, and showed time and concentration dependent; inhibit the expression of Bcl-2protein, activation of Caspase-3has the role of inhibiting the expression of Survivin; of integrinβ1,E-cd, β-catenin and the expression of c-myc inhibit anti-metastasis effect of play; also can inhibit b-FGF, MMP-9expression, high concentrations than low concentrations, and DDP combined with the best results.Conclusion:The in vitro cell culture experiments confirmed that realgar nanoparticles may inhibit A549cell proliferation and induce apoptosis, and play the role of multi-target anti-metastatic and anti-angiogenic efficacy for the treatment of lung cancer based on the challenged rule method provides the basic theory andlaboratory evidence; a lot of theory and clinical practice shows that attack toxic governance and effective treatment of lung cancer, with a good application prospect.