| Objective:1.In this dissertation, we select four acupuncture points of the aging model rats, respectively of dinghui,huoshuai, stomach and kidney, to investigate the curative effects of the three-points-group Mongolian moxibustion treatment on the ability of learning and memory, physical strength, weight change, thymus index and spleen index of the rats. Furthermore, the contents of DA, Ach, protein, and as well as the activity of MAO are observed to compare among the normal group, model group and moxibustion group to study the compatibility law of the three-points-group Mongolian moxibustion treatment for the anti-aging.2.Through the experimental investigation of the three-points-group moxibustion, we discuss the influencing mechanism on the nerve-endocrine-immunological network and histocyte, and the acting mechanism on the health care and anti-aging of the mongolian moxibustion treatment.Methods:A total number of60health SD rats of three months old with body weight of180±20g, in half male and half female, are divided into six groups (each contains10rats equally), respectively of the normal group, the model group, the dinghui-huoshuai-stomach points group, the dinghui-huoshuai-kidney points group, the dinghui-stomach-kidny points group and the huoshuai-stomach-kidney points group. For the normal and therapy groups, the rats in the model and therapy groups were given subcutaneous injection of5%D-galactose on the nape back (125mg/kg). The injection was performed in the morning (9:00-12:00) once daily for a period of42days. During the modeling period for the subacute aging rats, the body was weighted once a week and the changes of the fur colors, activities and diets were carefully observed. For the normal group, the rats were given only subcutaneous injection of 0.9%physiological saline on the nape back for42days. While setting treatment was performed on the rats in the normal and model groups, the moxibustion treatment was taken to the other groups starting from the seventh week. According to the available references, after the selection of the acupuncture points, we cut the fur and perform the moxibustion treatment with the covering of vaseline for protecting the skin and fixing the moxa cone. The moxa cones with the diameter of3mm, length of1cm and the cushion with the thickness of0.5mm were used in the treatments, which were lasted5min for each acupuncture point once a time, every other day. After the treatment, the learning ability and memory, body strength were evaluated and then the contents of DA, MAO, Ach and protein, and as well as the thymus and spleen indices were measured.Results:The change of the aging factors in the hippocampus shows that the contents of the DA, Ach, and protein and activity of MAO in the dinghui-huoshuai-kidney points group are not significantly different from those in the model group (P>0.05), however, other points groups show a significant increase in the content of the DA, Ach and protein with the obvious decrease in the MAO activities (P<0.01or P<0.05)The change in the thymus and spleen indices in the dinghui-huoshuai-kidney points group shows a similar trend with that in the model group (P>0.05), but other points group shows a significant increase of these indices when compared to the model group (P<0.01or P<0.05).The learning ability and memory capacity of the rats in the dinghui-huoshuai-kidney points group are comparable with those of the model group (P>0.05); however, other points group shows a significant increase in the learning ability and memory capacity when compared to the model group(P<0.01or P<0.05).The body strength of the rats in the dinghui-huoshuai-kidney points group are comparable with those of the model group (P>0.05); however, other points group shows a significant increase in the body strength when compared to the model group(P<0.01or P<0.05). Conclusions:1. Mongolian treatments of the three-points-group moxibustion can increase the contents of the DA, Ach and protein in the central neurotransmitter, and inhibit the MAO activity, which delays the recession of the cholinergic nervous system function and the biochemical change in the central cholinergic nervous system, and achieve the goals of preventing the aging of the central nervous system.2. Mongolian treatments of the three-points-group moxibustion can increase the thymus and spleen indices of the rats and then improve the body’s immunity and antioxidant capacity and achieves the anti-aging goals.3. Mongolian treatments of the three-points-group moxibustion can improve the rat’s ability of positioning navigation and space exploration, indicating that the anti-aging is realized through the improvement of the brain’s advanced functions, which is manifested by the learning ability and memory capacity.4. Mongolian treatments of the three-points-group moxibustion can prevent the D-galactose-based aging rat model’s chronic fatigue due to the swimming in the water, showing the function of the improving the body’s physical strength and health care.5. According to the current experimental results, the therapeutic effects of the A, B and C groups are multiple times of that of the single-point Moxibustion rather than their simple plus. There are following possible causes may responsible for the observation made after the combination of those specified acupuncture points:(i) considerably increased dissolution rate of the active ingredients,(ii) the synergy of the different ingredients, and (iii) the anti-aging mechanisms of each acupuncture point are realized through the the same passageway. The anti-aging effect of the D and M groups is insignificant. This may be correlated with the fact that the anti-aging mechanisms of the dinghui, huoshuai and kidney points are neutralized with the dynamic interactions. Therefore, the combination of these three acupoints is contraindicant in the clinical therapy. |
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