Refer To Dryness Syndrome Origin Analysis To Explore&Build Detailed Plan Of Animai Model In Northwest Dryness Syndrome Research

Objective: Basing on the discussion of Northwest Dryness Syndrome’s multiple essenceetiological factor and complicated internal syndrome structure, to explore the methods ofhow to build up animal model, including the experimental animal selection, experimentalintervention conditions, experimental factors combination and experimental processdesign, etc, forming a detailed plan of building Animal Model in order to lay thefoundation for further study.Methods:1) Basing on the systematic literature review of Dryness evil and DrynessSyndrome, investigating the origin and development of the theory, and analysising on thedifferences and relationship between Dryness Syndrome and Northwest DrynessSyndrome, revealing the multiple essence etiology of Northwest Dryness Syndrome andbuilding up the complicated internal syndrome system of Northwest Dryness Syndrome,finally provide the evidence for the research of Northwest Dryness Syndrome Model.2)According to the comprehensive analysis and comparison between the experimental cost,operating difficulties, and the various animals’ biological characteristics, selecting theideal experimental animal for establish the Northwest Dryness Syndrome’s model.3)Refer to the experimental methods and Syndrome-building-pattern of six evil study indomestic and select the appropriate Syndrome-building-pattern of Northwest DrynessSyndrome’s experiments4) Basing on the Six Evil’s factor analysis result of NorthwestDryness Syndrome’s external etiology, make sure that the main meteorological factor andits lever, which can be used to simulate the etiology of Northwest Dryness Syndrome.Refer to six evil study to select the appropriate experimental methods, which can be usedto explore the etiology of Northwest Dryness Syndrome, such as Dryness Evil, Heat Evil,Wine Evil and Cold Evil.5) Modifying the manual climatic box and Sand dust experimentbox, run them in order to simulate the Northwest Dryness Syndrome’s external etiology bycontrolling the factors of temperature, relative humidity, light intensity, wind and floating dust.6) Adapting mathematical methods to build up a climatic combination cycle of timeand space, making sure that the previous five factors would produce different levelsinteraction and application among Dryness Evil, Heat Evil, Wine Evil and Cold Evilduring a certain time (1week) and in certain space (manual climatic box, Sand dustexperiment box, and internal environment of animal laboratory).7) Basing on the analysisof the congruent relationship between the human’s syndrome and animal’s correspondenceand setting up the metrological syndrome discrimination.8) Under the guidance of thetheory of Western Medicine and TCM, from the perspective of the application of thedirection guidance method, simple screening method and metrological selecting method inproper order, select the appropriate indexes which can be used to conduct the observationand syndrome model evaluation.Results:1) Literature review reveals that Northwest Dryness Syndrome belongs to thebroad category of Dryness Syndrome, Northwest Dryness Syndrome is caused by thecombined evil of Dryness Evil, Heat Evil, Wine Evil and Cold Evil externally, also byanaphylactic body constitution internally. Northwest Dryness Syndrome does harm to lung,spleen, heart, kidney and other more viscera. Therefore, it would appear in internal andexternal Dryness Syndrome at the same time. Multiple etiology and complicatedsyndrome structure determine that the Northwest Dryness Syndrome is a multiple andgeographical regions Syndrome, which is different from the Normal Dryness Syndrome,such as seasonal characteristic Dryness Syndrome or Resemble Dryness Syndrome.2)Select the Etiology-model-building-pattern and from Main Syndrome of NorthwestDryness Syndrome to start the Northwest Dryness Syndrome’s animal model.3) Take thetemperature, relative humidity, light intensity, wind and floating dust for consideration asthe treatment factors to simulate the Northwest Dryness Syndrome’s etiology. Take theearth from Khotan to get the raw material as experimental dust, choose0.52,0.27,0.13,0.08.as the constituent ratio in determination of the intensity of Dryness unit, Heat unit,Wine unit and Cold unit in sequence.4) Take the temperature, relative humidity, lightintensity, wind and floating dust for consideration as the treatment factors, carry on it byapplying the manual climatic box, sand dust experiment box, and internal environment inanimals’ experiments.5) The relationship between the six evil and prosessing factor likethis four: Dryness-Qi-move-change(q1) presents temperature18℃, relative humidity20%,illumination60~80Lux, wind blown2h/d, dust4h/d; Heat-Qi-move-change(q2) presentstemperature30℃,relative humidity30%, illumination90~120Lux, wind blown2h/d, dust2h/d; Wine-Qi-move-change(q3) presents temperature18℃, relative humidity of30%, illumination90~120Lux, wind blown4h/d, dust2h/d; Cold-Qi-move-change(q4) presentstemperature6℃, relative humidity of40%, illumination60~80Lux, wind blown2h/d,dust1h/d;6) To set6d is a manmade climatic period, so Dryness Evil unit, Heat unit,Wine unit and Cold unit is3d,3d,2d and1d in sequence in the above time scope.7) Bodyweight, food and water intake, exploration competence, independent activity frequency,the maximum speed, orifice appearance, posture and excrement character with the nakedeye are chosen to be the indicators of animal Northwest Dryness Syndrome differentiation,different levels index-structure are the evidence of model evaluation in terms ofTraditional Chinese Medicine.8) Choose18indexes as the Microscopic observationindicators, such as SP-D expression of lung, β-defensin lever of nasal mucosa etc.Conclusions:1) Northwest Dryness Syndrome is a kind of multiple syndrome, which hasmultiple etiological factors and complicated pathogenesis, so the Syndrome can do harmto several viscera.2) Apply the etiological-model-building pattern and Syndrome-model-building pattern to establish the SD rat model of Northwest Dryness Syndrome.3)Etiological model-building pattern requires to simulate Kohtan’s climatic environment bycontrolling the temperature, relative humidity, light intensity, wind and floating dusttimely and spatially, so we need to simulate the main ecology of Northwest DrynessSyndrome in laboratory.4) Apply the metrological syndrome discrimination in carrying onthe animal’s Northwest Dryness Syndrome discrimination is a highly-efficient method toincrease the similarity between the animal experiment and clinical syndromediscrimination, and then achieve this transformation from the human beings to animal.5)Symdrome model-building pattern implements the transformation from human syndromediscrimination to animal syndrome discrimination as to Northwest Dryness Syndrome. Bythe way of mathematical formula, select18indexes from hundreds of indexes which areused by six evil animal model studying in domestic. It is a efficient and effectiveevaluation method by applying the direction guidance, simple screening, metrologicalselecting in proper order to select the appropriate indexes from the domestic Six Evilstudy.