| Insomnia is a series of psychiatric symptoms that a sleep onset and sleep maintenance disorder result in lack of sleep and can not meet the physiological needs of the organism. In Mongolian Medicine,it belongs to the Mongolian "Heyi" disease category.Using the western therapy still remain problems which are hardly to cure and different degrees of adverse reactions,drug resistance and dependence.The Mongolian Medicine Warm Acupuncture treatment can imprve the qulity of sleep through multi-level and various ways and methods. The most advantage is avoiding the side effects by the oral medication.Therefore,searching a proper,effctive acupuncture method is one of the key projects.Mongolian Medicine Warm Acupuncture can adjust unbalanced Heyi,can improve the flow of Heyi and Blood and can restore the harmonious blance of Mahbud. According to the recent study,the Mongolian Medicine Warm Acupucture can affect certain neurotransmitter levels and cytokin levels.ObjectiveFrom experiment research,to observe the influence of the sleeping time of rats induced by sodium pentobarbital and explicit that Mongolian Medicine Warm Acupuncture improving effect the sleep. Use the chronic intraperitoneal injection of amphetamine acid(PCPA) animal model of insomnia as the objects, put warm acupuncture on "Ori"."Heyi ","Zurih" acupuncture point and choose capability of behavioral changes of each group, changes of content of5-HT,5-HIAA,IL-1β,IL-2,IL-6, TNF-a as indicators. The object of this study is to explore the mechanism of using Mongolian Medicine Warm Acupuncture to cure insomnia.Methods①We chose30healthy male SD rats and random divided them into nonnal guoup and Mongolian Medicine Warm Acupuncture treatment group and15rats for each group, then treated the cure group with Mongolian Medicine Warm Acupuncture for7days. After that, observed the sleep time.②We chose60healthy male SD rats and random divided them into normal guoup, insomnia group, Mongolian Medicine Warm Acupuncture treatment group and diazepam injection group and15rats for each group. Used the chronic intraperitoneal injection of amphetamine acid(PCPA) animal model of insomnia, and then treated the cure groups with Mongolian Medicine Warm Acupuncture for7days.④Observe the rat’s jeneral status and free activities through open field test.⑤According to the HPLC method to measure the content of5-HT.5-HIAA in the cerebrum.⑥Using the ELISA method to measue the content of L-1β,IL-2,IL-6,TNF-a.Result1. Sleep time:Mongolian Medicine Warm Acupuncture treatment group can prolonge the pentobarbital sodium induced sleep time,compared with the normal group there were significant differences(P<0.01).2. Changes of body weight:In the third days and seventh days of insomnia, compared with the model group, Changes of body weight of Mongolian Medicine Warm Acupuncture treatment group were significant differences(P<0.05);compared with other groups, body weight of the rat of the nomal group.3. Behavioral changes:In open field test, the time of movement of rat of model group higher than normal group with highly significant differences(P<0.01);compared with model group, Mongolian Medicine Warm Acupuncture treatment group were significant differences (P<0.05).The time of inner zone of rat of model group more than control group> but there were not significant differences (P>0.05);compared with model group, Mongolian Medicine Warm Acupuncture treatment group were not significant differences (P>0.05).rats of model group made significantly more than normal group in stand-up times; Mongolian Medicine Warm Acupuncture treatment group less than model group in stand-up times(P<0.05).4.5-HT,5-HIAA content:From the study on the influence on5-HT,5-HIAA content of hypothalamus, hippocampus, pallium, brianstem showed that it can significantly increase the5-HT,5-HIAA content of hypothalamus, hippocampus of rats of Mongolian Medicine Warm Acupuncture treatment group. Which were significantly different than that of model group(P<0.05); it can not significantly increase the5-HT,5-HIAA of pallium, brianstem (P>0.05). 5. IL-1β content:From the study on the influence on IL-1β content of hypothalamus, hippocampus, pallium,brianstem showed that it can significantly reduce the IL-1β content of hypothalamus, pallium of rats of Mongolian Medicine Warm Acupuncture treatment group. Which were significantly different than that of model group(P<0.05); there is a little reduce the IL-1β of hippocampus, but there is not significantly different compared with that of model group (P>0.05).it not influence the IL-1β of brianstem of rats, there is not significantly different compared with that of model group (P>0.05).IL-2content:From the study on the influence on IL-2content of hypothalamus. hippocampus, pallium, brianstem showed that it can significantly reduce the IL-2content of hypothalamus、hippocampus、 pallium of rats of Mongolian Medicine Warm Acupuncture treatment group. Which were significantly different than that of model group(P<0.05, P<0.05, P<0.01);there is no influence the IL-2of brianstem of rats, there is not significantly different compared with that of model group (P>0.05).IL-6content:From the study on the influence on IL-6Content of hypothalamus, hippocampus, pallium, brianstem showed that it can significantly reduce the IL-6content of hypothalamus, hippocampus, pallium of rats of Mongolian Medicine Warm Acupuncture treatment group. Which were significantly different than that of model group (P<0.05, P<0.01, P<0.01); there is no influence the IL-6of brianstem of rats, there is not significantly different compared with that of model group (P>0.05).TNF-a content:From the study on the influence on IL-6Content of hypothalamus、 hippocampus、 pallium、 brianstem showed that it can significantly reduce the IL-6content of hypothalamus of rats of Mongolian Medicine Warm Acupuncture treatment group. Which were significantly different than that of model group(P<0.05);there is no influence the IL-6of hippocampus、 pallium、 brianstem of rats, there is not significantly different compared with that of model group (P>0.05).Conclusion1. Mongolian Medicine Warm Acupuncture can improve sleep, can prolong the sleeping time of rats indued by sodium pentobarbital.2. Mongolian Medicine Warm Acupuncture can reduce the spontaneous activity, improve behavioral effects as reaction. 3.5-HT,L-1β,IL-2,IL-6,TNF-a participate in improving sleep of Mongolian Medicine Warm Acupuncture.4. Effect of Mongolian Medicine warm acupuncture on the hypothalamus, hippocampus, cerebral cortex of insomnia rats was significant. Mongolian Medicine Warm Acupuncture could improve rat’s sleep,which might be possibly achieved through changing the5-HT content of hypothalamus, hippocampus and IL-1, IL-2, IL-6content of hypothalamus、 hippocampus and pallium.5.Mongolian Medicine Warm Acupunture with the advangates of simple oeration, safety,effective,few side effects. |
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