Study About Zedoray Turmeric Oil On Angiogenesis And Expression Of Related Regulatory Factors In Breast Precancerous Of Rats

Objective: the study evaluate reversal effect of Zedoray Turmeric oil on rat breastprecancerous lesions animal models, from rat survival of the state of the body, pathologicalmorphological aspects. From angiogenesis (microvessel density MVD, VEGF), withdetection method Histopathology, immunohistochemistry, in situ hybridization, exploreeffect and mechanism of the the Zedoray Turmeric oil to reverse breast precancerouslesions and Expression of Related Regulatory Factors VEGF mRNA in animal body of rats,in systemic, tissue, cell and molecular level. Methods：275SD rats were randomly dividedinto7groups: the blankcontrol group(BC),the precancerous lesion model controlgroup(PL),the kanglaite control group(KLT),the tamoxifen control group(TAM),the lowdose group of ZTO(ZTO-L),the middle dose group of ZTO(ZTO-M) and the high dosegroup of ZTO(ZTO-H). Animal model of precancerous lesion of rats mammary wasinduced bye DMBA and was treated by ZTO,KLT and TAM for4weeks. All the animalswere killed from8th to14th week once every two weeks. Observe breast shape,pathologydetection in each group, Determinate the microvascular markered with CD34density usingmmunohistochemical methods in the specimens,examination of histopathology wasperformed and measured the expression of VEGF mRNA by in situ hybridization. Results:1. The disease model group compared with the blank control group,rat mortality andincidence of precancerous lesions increased (P <0.05). the incidence of precancerous lesionof the high-dose group and the middle dose of Zedoray Turmeric oil reduced comparedwith disease module group and KLT group showed significant difference (P <0.05),compared with the tamoxifen control group show no significant difference (P>0.05).2.The positive rate of expression of VEGF mRNA and MVD trend to increase in every groups except BC corresponding to normal，usual ductal hyperplasia(UDH),atypical ductalhyperplasia(ADH) and invasive carcinoma.3. In ADH, The positive-cell rate of MVD ofZTO-M, ZTO-H and TAM was much lower than PL(P<0.05),the positive-cell rate ofVEGF mRNA of ZTO, KLT and TAM was much lower than PL(P<0.05),and thepositive-cell rate of VEGF mRNA of ZTO was much lower than KLT and TAM(P<0.05).In the case of ADH which the expression of VEGF mRNA was positive，the positive-cellrate of VEGF mRNA of ZTO-H was was much lower than ZTO-L(P<0.05). Conclusion:The breast precancerous lesion model induced by DMBA is able to replicate thepathological changes of the various stages in the process of mammary gland hyperplasia tocarcinoma. In the evolution process of simple mammary hyperplasia→breastprecancerous lesions→breast cancer, the MVD, VEGFmRna expression presentedsignificantly higher in the stage of precancerous lesions, this show that angiogenesis andrelated regulatory factor can reflect the vicious tendency of precancerous lesions of breastcancer. edoray Turmeric Oil decrease the expression of VEGF mRNA in precancerouslesion of rats mammary induced by DMBA, which could be the mechanism to inhibitsangiogenesis and prevent the breast cancer.