| In this paper,the cordyceps militaris was inoculated into the egg urinary cavity to successfully cultivate the egg cordyceps militaris culture,and the egg cordyceps militaris culture powder was prepared by spray drying.The morphology of egg cordyceps militaris culture was observed under the microscope and the mycelium of cordyceps militaris was consistent.Similar to cordyceps militaris,animal experiments can improve non-specific immunity,cellular immunity and humoral immunity in normal mice and significantly improve immunocompromised mice,improve egg quality in laying hens,improve serum biochemical parameters,and improve newcastle disease antibodies.Level,improve the utilization rate and economic benefits of laying hens during the laying period.1.Select the cordyceps militaris strain,inoculate 0.1ml,0.3ml,0.5ml in the 360-day-old pollution-free egg allantoic cavity and yolk sac cavity,inoculate 0.3ml strain in the allantoic cavity for 18 days of age.When the mycelium grows significantly,the spray-dried egg cordyceps militaris culture powder has a significant weight.2.Egg cordyceps militaris culture white grass after disinfection,crushing,beating,filtering and spray drying,taking the weight of egg cordyceps militaris culture powder as an indicator,using single factor and orthogonal test method to optimize the optimal process parameters affecting product index: inlet air temperature 150 ° C,into when the sample volume is 300 ml/h,the air flow rate is 600 l/h,and the desiccant ratio is 4%,the color is normal,and the egg cordyceps militaris culture powder weight is up to 7.59 g.3.Under the microscope,egg cordyceps militaris culture and the cordyceps militaris mycelium were consistent,and they were tubular and branched.The total genomic DNA was extracted by magnetic bead method,the primers of ITS region were designed,and the PCR amplification products were sequenced by two-way.The BLAST program and sequence analysis of various related strains were used to construct a developmental tree.The similar sequence of strain ZK-1 in GenBank was found.More,especially with cordyceps militaris strain CICC 14013(AY899259),the homology is as high as 100%,it is determined that the two strains are of the same species in taxonomy,belonging to cordyceps militaris.4.Egg cordyceps militaris culture white grass powder according to high,medium and low doses of stomach,body temperature,drinking water,body weight and other general conditions and normal mice are not obvious,High-dose egg cordyceps militaris culture significantly increased the spleen index,thymus index,clearance index,and phagocytosis index of normal mice.High-dose,medium-dose and low-dose egg cordyceps militaris culture increase feed intake and body weight in immunocompromised mice,proliferate immune organ cells,significantly increase the spleen index,thymus index,clearance index,and phagocytic index,and significantly improve the immune-impaired mice.The ability of monocytes to phagocytose enhances the non-specific immune function of mice.5.High-dose,medium-dose and low-dose egg cordyceps militaris cultureand positive drug mice significantly increased leukocytes compared to normal mice.Other indicators were not significantly different,and immunity to normal mice was improved.The white blood cells of high,medium and low doses of egg cordyceps militaris culture and positive drugs were significantly increased by 56.55%,42.77%,37.93% and 44.84%,and the number of neutrophils increased significantly by 127.29%,90.93%,63.65% and 109.11%,red blood cells increased significantly by 18.08%,14.94%,13.25%,9.01%,the average hemoglobin concentration decreased significantly by 3.75%,3.58%,2.12%,1.04%,and platelets increased significantly by 30.76%,31.38%,0.69%,34.65%.The lymphocytes of high and medium dose mice were positively increased by 20.45%,14.39%,and 15.91%,and the low dose did not increase significantly by 9.85%,indicating high,medium,and low doses of egg cordyceps militaris culture and positive.The drug significantly improved the cellular immune function of the immunocompromised mice,and the level of immune function regulation of the mice was comparable.There was no significant difference in blood parameters between the high,medium and low doses of egg cordyceps militaris culture.The high dose of egg cordyceps militaris culture could increase the cellular immunity of mice compared with the medium and low doses.6.Serum hemolysin and antibody-producing cells of high-dose egg cordyceps militaris culture mice were significantly increased,and compared with normal mice.Medium-dose antibody-producing cells increased significantly compared to normal mice,and high and medium-dose egg cordyceps cultures significantly increased humoral immune levels in normal mice.High-dose egg cordyceps sinensis cultures significantly increased serum hemolysin and significantly increased antibody-producing cells in immunocompromised mice;the serum hemolysin of the medium-dose egg cordyceps militaris culture has a significant increase of 14.87% compared to the immunocompromised mice,the antibody-producing cells of mice with medium and low doses of egg cordyceps militaris cultures increased significantly by 93.04% and 84.58% compared with those with low immunity,indicating that high-dose egg cordyceps militaris cultures significantly increased the humoral immune level of mice compared with positive drugs the dose of egg cordyceps militaris culture and the positive drug significantly increased the humoral immunity level of mice,and the high,medium and low doses of egg cordyceps militaris culture increased the humoral immunity level of mice.7.Adding egg cordyceps militaris culture powder to the feed of laying hens,the total protein and albumin increased significantly,triglycerides and cholesterol decreased,and high-density lipoprotein increased.The protein content is reduced,which promotes the full absorption and utilization of nutrients such as protein and fat in the body.The egg production rate and average egg weight increase significantly,and the sand egg is significantly reduced,which improves the production of eggs and eggs in the late laying period.The egg shape index improved significantly by 1.82%,the eggshell strength increased significantly by 4.37%,the eggshell weight increased significantly by 6.94%,the protein weight decreased significantly by 3.77%,the egg yolk color significantly increased by 6.55%,the protein height increased significantly by 3.08%,and the huff unit increased significantly 2.48%,egg freshness and uniformity increased,egg yolk color deepened,egg quality was significantly improved.The antibody titer of newcastle disease was significantly increased by 14.58%,which enabled the body to produce an effective immune response,which significantly improved the body's immune level.The use of egg cordyceps militaris culture as a feed additive for laying hens has certain economic value.To sum up,inoculating cordyceps militaris strains in eggs successfully cultivated the egg cordyceps militaris culture,and the egg cordyceps militaris culture powder was prepared by the spray drying process.The morphology and structure of egg cordyceps militaris culture were consistent with that of cordyceps militaris.The gene sequence was similar to that of cordyceps militaris.As a new immune enhancer and feed additive for animal production,egg cordyceps militaris culture production performance has broad application prospects. |
PDF Full Text Request