Experimental Study For The Influence Of Type 2 Diabetes Mellitus On The No Content In Prostate And Bladderissues In Rats With Benign Prostatic Hyperplasia

Objective: To explore the experimental study for the influence of type 2 diabetes mellitus (T2DM) on the NO content in prostate and bladder tissues for bladder outlet obstruction in rats with benign prostate hyperplasia. Methods: 40 male SD rats were randomly divided into four groups: BPH+T2DM group; T2DM group; BPH group and control group. The BPH model were established by subcutaneously injecting testosterone propionate in 28days in the neck. After feeding with high glucose and fat four weeks, STZ (50mg/kg) were injected by tail vein and established T2DM animal model; After the model successfully established, the rats were sacrificed and remove the prostate and bladder tissues to scale the weight and prostate weight index. The NO content in prostate and bladder tissues were detected by nitric acid deoxidized methods. Results: 1. The wet weight of prostate in four groups had statistical significance (F=10. 17, P<0. 05) . The prostate wet weight in BPH+T2DM group were obviously higher than that were in T2DM and control group, and had statistical difference (P<0. 05) ; The prostate wet weight in BPH group were increased than that were in control group (P<0. 05) . However, there were no statistical significance between BPH+T2DM group and BPH group (P>0. 05) . 2. The content of NO in prostate tissue in four groups showed statistical significance (F=10. 92, P<0. 05) . The content of NO in prostate tissue in BPH+T2DM group were markedly lower than that were in T2DM, BPH and control group (P<0. 05) . Meanwhile, the NO content in BPH was lower than in control group (P<0. 05) . However, there were no statistical significance between T2DM group and control group (P>0. 05) . 3. The NO content in bladder tissue in BPH+T2DM, T2DM and BPH groups were lower than in control group. Nevertheless, there was no statistical significance (P>0. 05) . Conclusions: The decreased content of NO in prostate tissue in BPH+T2DM group may reveal the down regulation of NO when BPH combined with T2DM, T2DM may be one of the cause leading the bladder outlet obstruction with the function of androgen; The decreased content of NO in prostate tissue in BPH group reveal that in male sex organ (the target organ of androgen) the NO content was highly affected by the androgen level.