| ObjectiveBased on the idea of "preventing disease" with acupuncture,this study firstly screened the early stress-induced gastric mucosal lesions(SGML)by comparing the effects of water-immersion restraint stress(WRS)on the morphological structure and function of gastric mucosa in rats.Then,the effects of electroacupuncture preconditioning at Zusanli acupoint on the morphological structure and function of gastric mucosa in early SGML rats and the distribution,number and degranulation of mast cells(MCs)in the acupoint area were observed.Subsequently,the correlation between the protective effect of electroacupuncture preconditioning at Zusanli acupoint on gastric mucosa in early SGML rats and Mast cells in Zusanli acupoint was evaluated.the correlation between the protective effect of electroacupuncture preconditioning at Zusanli acupoint on gastric mucosa in early SGML rats and Mast cells in Zusanli acupoint.On this basis,acupuncture sera of rats preconditioned with electroacupuncture at Zusanli acupoint were extracted and used for in vitro culture of rat normal gastric mucosal epithelial cell line RGM1,and the effect of different sources of rat acupuncture serum on the function of gastric mucosal epithelial cell line RGM1 was compared with the acupuncture serum of SCG group.Comprehensive analysis of the above in vivo and in vitro experimental results,to explore the protective effect and mechanism of electroacupuncture preconditioning at Zusanli acupoint on gastric mucosa in rats with early SGML,to provide experimental basis for clinical application of electroacupuncture to prevent early SGML related diseases.Method40 SD male rats were divided into 4 groups according to the random number table: normal control group(WRS 0min group),WRS for 10 min group(WRS 10 min group),WRS for 30 min group(WRS 30 min group),WRS for 60 min group(WRS 60 min group),10 rats in each group.The rats in each group were taken out of the water immediately after the end of the WRS for the corresponding period of time,and cardiac perfusion fixation was performed.The p H value of gastric juice of rats was measured;the gross damage of gastric mucosa was observed with naked eyes and the gastric mucosal damage index(DI)was counted.After HE staining,the morphological structure of gastric mucosa in rats were observed under light microscope and histopathological scores were calculated;after PAS staining,the content of gastric mucosal mucus was observed under light microscope and semi-quantitative of gastric mucosal mucus was performed.48 SD rats were divided into 4 groups according to the random number table: normal control(Control group): Except for the same grasping and immobilization as the EA group for consecutive 7 days,no other treatment was performed,n=12.WRS for 10 min group(WRS group): The rats in the EA group were grasped and immobilized in the same way as the EA group for consecutive 7 days,and the WRS was performed on the 8th day,n=12.Electroacupuncture preconditioning + WRS modeling group(EA+WRS group): firstly,electroacupuncture preconditioning at bilateral Zusanli acupoint was performed for consecutive 7 days,and WRS for 10 min was performed 24 hours after the last electroacupuncture preconditioning,n=12.The only electroacupuncture preconditioning group(EA group): Except for electroacupuncture preconditioning at bilateral Zusanli acupoint for consecutive 7 days,no other treatment was performed,and the tissues were directly collected 24 hours after the last electroacupuncture preconditioning.n=12.In each group,6 rats were randomly selected for cardiac perfusion fixation,and the gastric tissue and Zusanli acupoint tissue were taken for postfixation;the remaining 6 rats were used for scraping gastric mucosa and obtaining skin and subcutaneous tissue in the acupoint area in the living organism.First,the general condition of gastric mucosal injury was observed with the naked eye and the gastric mucosal DI was counted;the histopathological changes of the gastric mucosa of the rats were observed under the light microscope after HE staining and the histopathological score was calculated;the gastric mucosal mucus of the rats was observed under the light microscope after PAS staining.Toluidine blue staining was used to observe the distribution,number and morphology of MCs in gastric mucosa,and the number and degranulation rate of MCs were counted.After immunohistochemical staining,the distribution and number of gastric mucosal Mast Cell-Tryptase positive cells were observed under light microscope,and the positive cell rate was counted;ELISA was used to detect the levels of inflammatory factors IL-33,TNF-?,IL-6,L-17 and the content of 5-HT and HA in gastric mucosal tissue;Western blot was used to detect the expression levels of NF-?B signaling pathway related proteins p-I?B?(Ser32),I?B?,pNF-?B(Ser468),NF-?B in gastric mucosa.Then,the distribution,number and morphology of MCs in Zusanli acupoint were observed by toluidine blue staining,and the number of MCs and degranulation rate were counted;ELISA was used to detect the content of 5-HT and HA in Zusanli acupoint tissue.Finally,the Pearson correlation coefficient was used to analyze the correlation between the degranulation rate of MCs in Zusanli acupoint area and the gastric mucosal injury index DI,gastric mucosal histopathological score,and the content of gastric mucosal mucus respectively.48 SD rats were randomly divided into 2 groups according to the random number table: One group was given bilateral Zusanli acupoints injection of normal saline(NS);the other group was given bilateral acupoints injection of the mast cell membrane stabilizer Sodium cromoglicate(SCG).These two groups both included two subgroups,namely,the WRS modeling group and the electroacupuncture preconditioning + WRS group,with a total of 4subgroups: NS+WRS group: 10?l of NS was injected into the bilateral Zusanli acupoint of the rats every day from 1st to 7th day,followed by WRS for 10 min for modeling on the 8th day,n=12.NS+EA+WRS group: from day 1 to day 7,rats were injected with 10 ?l of NS at the bilateral Zusanli acupoints each day,and half an hour later,the rats were preconditioned with electroacupuncture at bilateral Zusanli acupoints,n=12.SCG+WRS group: from day 1 to day 7,10?l of SCG was injected into the bilateral Zusanli acupoints of the rats every day,and on day 8,WRS was performed for 10 min,n=12.SCG+EA+WRS group: from day 1 to day 7,rats were injected with 10?l of SCG at the bilateral Zusanli acupoints of the rats every day,and half an hour later,the rats were preconditioned with electroacupuncture at bilateral Zusanli acupoints,and on day 8,WRS was performed for 10 min,n=12.Six rats in each group were randomly selected for cardiac perfusion fixation,and Zusanli acupoints tissues and stomach tissue were collected for subsequent fixation.The remaining 6 rats were used to scrape gastric mucosa in the living organism.Toluidine blue staining was used to observe the distribution,number and morphology of MCs in Zusanli acupoints and gastric mucosa,and the number and degranulation rate of MCs were calculated.The general situation of gastric mucosa injury was observed by naked eye and the gastric mucosa DI was counted.The histopathological changes of gastric mucosa of rats were observed under light microscope after HE staining and the histopathological scores were counted.After PAS staining,the content of gastric mucus was observed under light microscope.The levels of IL-33,IL-6,IL-17,TNF-? and the contents of 5-HT and HA in gastric mucosa were detected by ELISA.40 SD rats were randomly divided into 4 groups according to the random number table,with 10 rats in each group: NS+Control group: 10?l of NS was injected into bilateral Zusanli acupoints of the rats,once a day for 7consecutive days.NS+EA Group: The rats were injected with 10?l of NS into bilateral Zusanli acupoints of the rats,and half an hour later,they were precondioned with electroacupuncture on the bilateral Zusanli acupoints,once a day,for 7 consecutive days;(3)SCG+Control group: the rats were injected with bilateral Zusanli acupoints were injected with 10?l of SCG,once a day,for 7 consecutive days;(4)SCG+EA group: rats were injected with 10 ?l of SCG at the bilateral Zusanli acupoints,half an hour later,the bilateral Zusanli were preconditioned with electroacupuncture,once a day,for 7 consecutive days sky.24 h after the corresponding treatment,the rats in each group were collected blood from the apex of the heart,and serum was extracted,sterilized by filtration through a 220 nm filter membrane,and inactivated at 56°C for use.The normal rat gastric mucosa epithelial cell line RGM1 was cultured in vitro,and the extracted rat serum was used to culture RGM1 in vitro.RGM1 cells were divided into 4 groups according to the rat serum received from different groups: NS+C+R group: RGM1 cultured with serum from NS+Control group;NS+EA+R group: NS+EA group with acupuncture RGM1 cultured in serum;SCG+C+R group: RGM1 cultured in serum from rats in SCG+Control group;SCG+EA+R group: RGM1 cultured in acupuncture serum from rats in SCG+EA group.MTT colorimetric method was used to screen the optimal concentration of RGM1 in rat serum in vitro;trypan blue staining was used to calculate the survival rate of RGM1 cells;PAS staining was used to observe the mucus secretion capacity of RGM1;ELISA was used to detect the level of inflammatory factors IL-33,TNF-?,IL-6;Western blot was used to detect the protein expression levels of p-I?B?(Ser32),I?B?,p-NF-?B(Ser468),NF-?B in RGM1 cells.Result1 Effects of different durations of water-immersion restraint stress on morphological structure and function of gastric mucosa in rats(1)General view of gastric mucosa: Compared with the WRS 0min group,the gastric mucosa of the rats in the other three groups showed different degrees of damage,ranging from mucosal edema and scattered punctate hemorrhages on the surface in mild cases,to bleeding,erosion and even ulcer in severe cases.Statistical analysis showed that with the increase of WRS time,the gastric mucosal damage index DI increased(P<0.01,P<0.01,P<0.05).(2)pH value of gastric juice: The differences in p H of gastric juice between the four groups were statistically significant.Although,compared with the WRS 0min group,the gastric p H value of the rats in the WRS 10 min group did not change significantly.However,compared with the WRS 0min and WRS 10 min groups,the p H of the gastric juice of the rats in the WRS30 min group was significantly lower(P<0.01,P<0.01).values were significantly lower(P<0.01,P<0.05).(3)Histopathological changes of gastric mucosa: No obvious pathological changes were found in the gastric mucosa of rats in the WRS0 min group.In the WRS 10 min group,the gastric mucosal epithelium was incomplete,ruptured,and epithelial cells were swollen and degenerated;the glands in the lamina propria were sparse and disordered;the small blood vessels in the submucosal layer were clearly visible,with vasodilation and congestion,and red blood cell exudation;Inflammatory cell infiltration.In the WRS 30 min group,the gastric mucosal epithelium was shed,the thickness was reduced,and the epithelial cell degeneration was increased;the glands were sparse and disorderly arranged;the submucosa was congested and edematous,there were obvious red blood cell exudation in the blood vessels,and the local inflammatory cell infiltration in the mucosa was aggravated.In the WRS 60 min group,the gastric mucosal epithelium was obviously detached and the mucosa was necrotic;the lamina propria glands were obviously atrophied and the number was reduced;the submucosa hyperemia and edema were aggravated,the intravascular red blood cell exudation was aggravated,the mucosa was infiltrated by a large number of inflammatory cells,and the damage was deep to the muscle Floor.Compared with the WRS 0min group,the histopathological scores of the gastric mucosa of the other three groups were significantly increased(P<0.01,P<0.01,P<0.01),and with the increase of the WRS time,the pathological tissue of the rats increased significantly(P<0.01,P<0.01,P<0.01).The higher the academic score(P<0.05,P<0.01).(4)The content of gastric mucosal mucus: The gastric mucosal epithelium of the rats in the WRS 0min group was covered with a thick and continuous layer of mucus,which was stained into clusters of purple-red strips by PAS stain;Packed tightly with cytoplasm at the top and filled with mucin granules.The gastric mucosal epithelium of the rats in the WRS 10 min group was incomplete and occasionally ruptured;the surface mucous cells were swollen and degenerated;the amount of mucosal mucus was reduced.The gastric mucosal epithelium of the rats in the WRS 30 min group was incomplete,and mucosal gaps were seen in many places;the surface mucous cells were degenerated,decreased in number,and sparsely arranged;the amount of mucosal mucus was significantly reduced.The gastric mucosal epithelium of the rats in the WRS 60 min group fell off,the surface mucous cells were absent,and the mucous layer disappeared.Compared with WRS0 min,the content of gastric mucosal mucus in the other three groups was significantly decreased(P<0.01,P<0.01,P<0.01);and,with the prolongation of WRS,the content of gastric mucosal mucus decreased more land(P< 0.01,P< 0.05).2 The effect of electroacupuncture preconditioning at Zusanli acupoint on the morphological structure and function of gastric mucosa in rats with early SGML(1)General view of gastric mucosa: The gastric mucosa of the rats in the Control group was smooth,ruddy in color,and no obvious bleeding spots and erosions were found.Compared with the Control group,the gastric mucosal DI of the WRS group was significantly increased(P<0.01);compared with the WRS group,the gastric mucosal DI of the EA+WRS group was significantly decreased(P<0.01).(2)Histopathological changes of gastric mucosa: The morphology and structure of gastric mucosa of rats in the Control group and EA group were intact,with clear layers,and no obvious histopathological changes were found in each layer of the mucosa.In the WRS group and the EA+WSR group,the gastric mucosal epithelium was incomplete and ruptured.The gastric mucosal glands were atrophied and disordered to varying degrees.Different degrees of inflammatory cell infiltration.Compared with the Control group,the gastric mucosal histopathological score of the WRS group was significantly increased(P<0.01);compared with the WRS group,the gastric mucosal histopathological score of the EA+WRS group was significantly decreased(P<0.05).(3)Changes of the content of gastric mucosal mucus: Compared with the Control group,the gastric mucosal mucus volume in the WRS group was significantly decreased(P<0.01);compared with the WRS group,the gastric mucosal mucus volume in the EA+WRS group was significantly increased(P<0.05).(4)Changes of the number and degranulation rate of gastric mucosal MCs: Compared with the Control group,the number and degranulation rate of gastric mucosal MCs in the WRS group were significantly increased(P<0.01,P<0.01);The number and degranulation rate of gastric mucosal MCs in the EA+WRS group were significantly decreased(P<0.01,P<0.01).The results of immunohistochemical staining showed that compared with the Control group,the rate of Mast Cell-Tryptase positive cells in the gastric mucosa of the rats in the WRS group was significantly increased(P < 0.01);The rate of Tryptase positive cells was significantly decreased(P<0.05).The results of ELISA showed that,compared with the Control group,the contents of 5-HT and HA in the gastric mucosa of the rats in the WRS group were significantly increased(P<0.05,P<0.05);the contents of 5-HT and HA in mucosal tissue were significantly reduced(P<0.01,P<0.01).(5)Changes of the levels of inflammatory factors in gastric mucosa:Compared with the control group,the contents of IL-33,TNF-?,IL-6 and IL-17 in the gastric mucosa of the WRS group were significantly increased(P<0.01,P < 0.05,P < 0.01,P < 0.01).Compared with the WRS group,the contents of IL-33,TNF-?,IL-6 and IL-17 in the gastric mucosa of the EA+WRS group were significantly decreased(P<0.05,P<0.05,P<0.05,P<0.01).(6)Changes of the expression of NF-?B signaling pathway-related proteins in gastric mucosa: Compared with Control group,the value of cytoplasmatic protein P-I ?B?(Ser32)/ IKB? in WRS group was significantly increased,with statistical significance(P<0.01);the expression of cytoplasmic protein NF-?B was significantly decreased,with statistical significance(P<0.01);the expression of p-NF-?B(Ser468)was significantly increased,and the difference was statistically significant(P<0.01).Compared with WRS group,the plasma protein p-I?B?(Ser32)/ IKB? value in EA+WRS group was significantly decreased(P<0.01);the expression of cytoplasmic protein NF-?B was significantly increased,with statistical significance(P<0.01);the expression of p-NF-?B(Ser468)was significantly decreased,and the difference was statistically significant(P<0.01).3 The relationship between the protective effect of electroacupuncture preconditioned at Zusanli on gastric mucosa in early SGML rats and the mast cells in the acupoint tissue(1)Changes of the number of MCs and degranulation in the Zusanli acupoint tissue: Compared with the control group,the number of MCs and the degranulation rate in the Zusanli acupoint tissue of the rats in the EA group increased significantly(P< 0.01,P< 0.05),as well as the content of 5-HT and HA(P< 0.01,P< 0.01);compared with the WRS group,the number of MCs and the degranulation rate in the Zusanli acupoint tissue of the rats in the EA+WRS group were also significantly increased(P< 0.05,P< 0.01),and the contents of 5-HT and HA were also significantly increased(P< 0.01,P< 0.01).(2)Correlation analysis: Pearson correlation coefficient was used to analyze the correlation between the degranulation rate of MCs in the Zusanli acupoint tissue and the gastric mucosal DI,gastric mucosal histopathological score,and the content of gastric mucosal mucus IOD(sum)/Area(sum).The results showed that there was a negative linear correlation between MCs degranulation rate and gastric mucosa injury index DI,and the correlation coefficient was r =-0.9063(P< 0.0001).There was a negative linear correlation between MCs degranulation rate and gastric mucosa histopathological score(r =-0.8765,P< 0.0001).There was a positive linear correlation between MCs degranulation rate and gastric mucous volume,and the correlation coefficient was r = 0.9017(P< 0.0001).4 Effect of injection of mast cell membrane stabilizer at Zusanli acupoint on gastric mucosa protection by electroacupuncture preconditioning(1)The number of MCs and degranulation in the acupoint tissue:Compared with the NS+WRS group,the number of MCs and the degranulation rate in the Zusanli acupoint tissue of the rats in the SCG+WRS group were significantly reduced(P<0.05,P<0.01);Compared with the +EA+WRS group,the number of MCs and the degranulation rate in the Zusanli acupoint tissue of the rats in the SCG+EA+WRS group were also significantly reduced(P<0.01,P<0.01).(2)General view of gastric mucosa: The gastric mucosa of rats in the NS+EA+WRS group had a smooth surface with only a few bleeding points,and the damage was relatively minor;the other three groups of rats had more gastric mucosal bleeding points,in the form of dots or strips.Bulk-like,severe damage.Compared with the NS+WRS group,the gastric mucosa DI of the NS+EA+WRS group was significantly decreased(P<0.01);compared with the NS+EA+WRS group,the gastric mucosa DI of the SCG+EA+WRS group was significantly increased(P<0.01).(3)Histopathological changes of gastric mucosa: the morphological structure of gastric mucosa of rats in NS+EA+WRS group was relatively complete,the mucosal epithelium was more continuous,and a small amount of epithelial cell degeneration and necrosis were occasionally seen;the lamina propria glands were regularly arranged,and the submucosa Mild edema,a small amount of erythrocyte exudation in the submucosal blood vessels,and scattered inflammatory cell infiltration in the mucosa;the other three groups of rats showed more serious tissue morphological damage than the NS+EA+WRS group.Compared with NS+WRS group,the histopathological score of gastric mucosa of rats in NS+EA+WRS group was significantly lower(P<0.01);compared with NS+EA+WRS group,the gastric mucosa of rats in SCG+EA+WRS group Histopathological scores were significantly increased(P<0.01).(4)Changes of the content of gastric mucosal mucus: The gastric mucosal epithelium of the rats in the NS+EA+WRS group was occasionally broken,and the mucous layer covered by the mucosal epithelium was stained purple-red by PAS dye,while the gastric mucosal mucus appeared in the other three groups.varying degrees of reduction.Compared with the NS+WRS group,the amount of gastric mucosal mucus in the NS+EA+WRS group was significantly increased(P<0.05).Significantly decreased(P< 0.01).(5)Changes of the number of gastric mucosal MCs and degranulation:Compared with the NS+WRS group,the number of gastric mucosal MCs,the degranulation rate and the content of 5-HT and HA in the NS+EA+WRS group were significantly increased(P<0.01,P<0.01;P<0.01;P<0.05);compared with the NS+EA+WRS group,the number of MCs and HA content in the gastric mucosa of the SCG+EA+WRS group were significantly decreased(P<0.05;P<0.05),the MCs degranulation rate and 5-HT content had no significant difference between the two.(6)Changes of the levels of inflammatory factors in gastric mucosa:Compared with the NS+WRS group,the contents of IL-33,TNF-?,IL-6 and IL-17 in the gastric mucosa of the NS+EA+WRS group were significantly higher.Compared with the NS+EA+WRS group,the content of IL-33,TNF-?,IL-6 in the gastric mucosal tissue were significantly increased(P<0.05,P<0.01,P<0.05),but there was no significant difference in the content of IL-17 between the two groups.Compared with the SCG+WRS group,the content of IL-17 in the gastric mucosa of the rats in the SCG+EA+WRS group was significantly decreased(P<0.05),but the contents of IL-33,TNF-? and IL-6 in the gastric mucosa of the two groups were not significantly different.5 Effects of acupuncture serum on the function of gastric mucosal epithelial cells RGM1(1)MTT colorimetric method to screen the optimal concentration of acupuncture serum for in vitro culture of RGM1: MTT assay results showed that the concentration of 20% rat acupuncture serum had the greatest effect on the proliferation of RGM1 cells,and its OD value was 1.555±0.03,The OD values under the intervention of other concentrations were significantly decreased(P<0.01,P<0.01,P<0.01,P<0.01).(2)Survival rate of RGM1 cells: The survival rate of RGM1 cells was calculated after trypan blue staining.One-way ANOVA showed that there was no significant difference in the survival rate of RGM1 cells among the four groups(F=0.3779,P=0.7699).(3)Mucus secretion of RGM1: RGM1 in NS+EA+R group were stained darker,the nucleus was blue,the cytoplasm was stained dark red,the cell membrane was surrounded by thick red stains,the intercellular space was not clear,visible Crimson clumps;there is little difference in staining among the other three groups,all of which are lighter than the NS+EA+R group,the nucleus is blue,the cytoplasm is light red or pink,and the cells are surrounded by light red stains,the intercellular space is clear.Compared with the NS+C+R group,the mucus secretion of the cells in the NS+EA+R group was significantly increased(P<0.05);compared with the NS+EA+R group,the mucus secretion of the SCG+EA+R group was significantly decreased(P<0.05).(4)The release of inflammatory factors of RGM1: Compared with the NS+C+R group,the content of IL-33 and IL-6 in the cell culture supernatant of the NS+EA+R group were significantly decreased(P<0.05,P<0.05);Compared with the NS+EA+R group,the contents of IL-33 and IL-6 in the culture supernatant of the SCG+EA+R group were significantly increased(P<0.05,P<0.05);however,there was no significant difference in the content of TNF-? among the four groups.(5)Expression of NF-?B signaling pathway-related proteins in RGM1cells: Compared with NS+C+R group,the value of RGM1 cytoplasmic protein P-I ?B?(Ser32)/I?Ba in NS+EA+R group was significantly decreased(P <0.01);the expression of cytoplasmic protein NF-?B was significantly increased,with statistical significance(P < 0.01);meanwhile,the expression of p-NF-?B(Ser468)was significantly decreased,the difference was statistically significant(P < 0.01).Compared with NS+EA+R group,the plasma protein p-I?B?(Ser32)/I?Ba value in SCG+EA+R group was significantly increased,and the difference was statistically significant(P <0.01);the expression of cytoplasmic protein NF-?B was significantly decreased,with statistical significance(P < 0.01);meanwhile,the expression of p-NF-?B(Ser468)was significantly increased,and the difference was statistically significant(P < 0.01).Conclusion(1)Water-immersion restraint stress for 10 min can successfully induce the early SGML rat model with shallow lesion and mild damage.(2)electroacupuncture preconditioning at Zusanli can reduce the damage of gastric mucosal morphological structure in early SGML rats,increase gastric mucosal mucus volume,reduce the number and degranulation of gastric mucosal MCs,inhibit the activation of gastric mucosal NF-?B signaling pathway,and reduce the release of inflammatory factors IL-33,TNF-?,IL-6,and IL-17 in gastric mucosa,exerts a protective effect on gastric mucosa in early SGML rats.(3)Electroacupuncture preconditioning at Zusanli can increase the number of MCs and degranulation in the acupoint tissue;and the degranulation rate of MCs in Zusanli acupoint tissue has a linear negative correlation with gastric mucosal DI and gastric mucosal histopathological score,and has a linear postive correlation with gastric mucosal mucus.These results suggested that the protective effect of electroacupuncture preconditioning at Zusanli on gastric mucosa of early SGML rats was related to the activation of mast cells in acupoint area by electroacupuncture preconditioning(4)Injection of mast cell membrane stabilizer SCG at Zusanli acupoint can inhibit MCs aggregation and degranulation in acupoint area caused by electroacupuncture preconditioning,reduce gastric mucosal mucus increase caused by electroacupuncture preconditioning,weaken the inhibition effect of electroacupuncture preconditioning on gastric mucosal MCs aggregation in early SGML rats,and increase the release of gastric mucosal inflammatory factors.Thus,the protective effect of electroacupuncture preconditioning at Zusanli on gastric mucosa of early SGML rats was weakened.These results suggested that the degranulation of mast cells in the Zusanli acupoint was involved in the protective effect of electroacupuncture preconditioning on gastric mucosa of early SGML rats.(5)The acupuncture serum of rats preconditioned with electroacupuncture at Zusanli acupoint can increase the mucus secretion of RGM1,inhibit the activation of NF-?B signaling pathway in RGM1 cells,and reduce the release of inflammatory factors in RGM1,thereby exerting the protective effect of gastric mucosa epithelial cells;and the protective effect of acupuncture serum on RGM1 was weakened after acupoint injection of mast cell membrane stabilizer SCG.(6)Based on the above in vivo and in vitro experimental results,this study found that electroacupuncture preconditioning at Zusanli can induce the aggregation and degranulation of mast cells in the acupoint area,and produce bioactive substances with complex components into the blood,which are transported to the gastric tissue along the body fluid pathway,then directly acts on gastric mucosal epithelial cells,promotes the release of gastric mucosal epithelial cell mucus,inhibits the activation of NF-?B signaling pathway in gastric mucosal epithelial cells,and reduces the release of inflammatory factors in gastric mucosa,thereby protecting the gastric mucosa in early SGML rats. |
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