| Environmental hormones are environmental chemical substances, which can interrupt organism hormones secretion and function, and handicap the function of procreation enginery, neural system and immune system. Chemiluminescence is defined as some moleculars of reaction systems, such as reactants, productions or intermediates transition from ground state to excited state because of absorption in the energy by releasing from reaction, and in the process of returning to the ground state, the energy is released as a form of ray radiation and brings chemiluminescence. We added the Ag nanoparticles in the chemiluminescence system of the luminol species, and use these systems to detect the environmental hormones. The main reaserch contents include as follows:1. We found that Ag NPs could enhance the chemiluminescence intensity of luminol-potassium ferricyanide system. On the basis of enhancement effect, a flow injection method was developed for the determination of diethylstilbestrol. The structure and shape of Ag NPs were characterized by transmission electron microscopy (TEM). A possible mechanism of Ag NPs strengthening on luminol-potassium ferricyanide CL reaction was also discussed by the UV-Vis absorption spectra and chemiluminescence spectra. In the optimum conditions, the linear range was 6.0×10-8 1.0×10-6 g/L(r = 0.9960) and the detection limit was 4×10-8 g/L. The relative standard deviation was 2.6 % for 5.0×10-7 g/L diethylstilbestrol (n = 11). The recovery was between 98.0 % 102.0 %.2. It was found that Ag NPs could enhance the chemiluminescence intensity of luminol-potassium permanganate system. On the basis of enhancement effect, a flow injection method was developed for the determination of hydrocortisone, and a possible mechanism was also discussed. In the optimum conditions, the linear range was 3.0×10-10 7.0×10-8 g/L(r = 0.9987) and the detection limit was 1×10-10 g/L. The relative standard deviation was 2.1 % for 1.0×10-8 g/L hydrocortisone (n = 11). The recovery was between 98.0 % 103.0 %.3. It was found that Ag NPs could enhance the chemiluminescence intensity of luminol-hydrogen peroxide system. On the basis of enhancement effect, a flow injection method was developed for the determination of dexamethasone, and a possible mechanism was also discussed. In the optimum conditions, the linear range was 1.0×10-8 1.0×10-6 g/L(r = 0.9973) and the detection limit was 1×10-9 g/L. The relative standard deviation was 2.8 % for 1.0×10-7 g/L dexamethasone (n = 11). The recovery was between 98.0 % 101.0 %.
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