Studies Of Rhodiola Extracting Mehtod And Its Preparations

In the thesis, Salidroside, as the main component of Rhodiola ,is extracted and its complex with phospholipid is prepared.The research focuses on the preparation of refined extract and complex,the absorption and tissue distribution characteristics in animals .In order to provide a scientific basis for the development and application of this study ,a series of experiments have been done in it. Up to now, it is not reported on Salidroside-Phospholip Complex .HPLC method was developed in invitro and invivo analysis for assay during the study of preparation and pharmacokinetics.On the extraction of Rhodiola, the conditions were optimized by the orthographic design among the folio whig effecting factors: extracting solvent, extracting time and times. According to the properties of the active components, Column Chromatograghy of AB-8 resin and polyamide and ultrafiltration were used to refine the crude extract, which greatly improved the accumulation of Salidroside, lowed the impurities and guaranteed the transparence and stability of the injection.New attempts are made to find the reaction condition of Salidroside-Phospholip Complex, and a reasonable criterion of accurate evolution was established, by which such factors as temperature, the ratio of Salidroside to phospholipid and the concentration of reactant are examined .Theorthographic design is used in the optimization of reaction condition and the best are obtained through statistical analysis and proof test. Some differences of apparent oil/water partition coefficients in systems consisted of different pH water and n-octyl alcohol are found between the complex and salidroside. It is shown that a great improvement of apparent solubility of salidroside-phospholipid complex both in water and in n-octyl alcohol.The pharmacokinetics after injection of an equivalent dosage of salidroside and its complex are compared by cross test design. The results demonstrate that the concentrations of salidroside in rat and mouse plasma after injection intake of them are different. Further research shows that the concentrations of in heart and brain after injection complex are higher than the those of free drug on the tissue distribution of the complex hi mouse.