Experimental Study On Formula Jiaotai Pill By Semi-Bionic Extraction

Objective: Optimizing the extract technology in the modification of the formula of Jiaotai Pill and further verifying the correctness and practicality of semi-bionic extraction(SBE). Methods: At first, optimizing the SBE technical conditions for Jiaotai Pill ingredients through even design while berberine, cinnamic acid, total alkaloids, volatile oil and dry extract were adopted as indexs; Then, dividing the ingredients into 2 combinations and extracting by the SBE method respectively, determining the contents of index components in the each extract, comparing the TLC of absolute methanol solution and the GC of volatile oil, and making out the SBE s best ingredients combination For the formula of Jiaotai Pill. The SBE extract of the optimized ingredients combination was put to further comparison of components in different conditions of alcohol precipitating concentrations, confirming the most ideal alcohol precipitating concentration of the semi-bionic extraction by precipitation with alcohol (SBAE) for the formula. On this base, comparison of components were conducted over the four extracts derived from four different kinds of extraction methods that are the SBE, the water extraction(WE), SBAE, and the water extraction by precipitation with alcohol (WAE), and the mitigtive functions of the extracts of SBE and WE were compared. Results: The technical condition of SBE for the formula of Jiaotai Pill was: The pH value in first extraction was adjusted to 2.20 by the use of HCL solution. And then the pH values in the second and third extraction should be adjusted to 6. 50 and 8.70 by the use of NaOH solution, the durations ofeach section were 170min, 80min and 40min, respectively. it is better to extract Rhizoma Coptidis and Cortex Cinnamomi seperately. The comprehensive values assessed by the contents of 5 index components in the four extracts were : SBE >WE>SBAE>WAE. The mitigtive function of SBE extract is obviously more effective than that of WE. Conclusion: SBE method is the best among the four methods in the extraction of Jiaotai Pill components. The study firmly verifies that the SBE method is correct and practical, consummates the basic study model of Traditional Medicine compound s modification by using the SBE method , provides some experiences for Traditional Medicine s matching study.