| Luminescence analysis is the abbreviation of molecular spectrum, including molecuar fluorecence phosphorescence and chemliluminescence analysis (including bioluminescence) .Chemiluminescence analysis is a trace analysis method which determines the content of substance according to the light emission of the chemical reaction. In 1877, Radziszewski found that lophine emmitted green light when it reacted with oxygen in the prescence of a base.This is the first example of chemiluminescence using a synthetic organic compound. There are gas phase, flame and liquid chemiluminescence reaction systems, chemiluminescence analysis can has high sensitivity (Detect limit can be 10-12-10-18mol/L), wide linear range (three to six magnitude order) and simpler, lower instruments. Chemiluminescence method with coupling reaction, chemiluminescence immunoassay which has developed for recent ten years is the popularization of chemiluminescence technology. For the above advantages, Chemiluminescence has been widely applied in environment science, biological science and clinical science.Fluorescence emits when the electrons transfer from the fisrt excited single state to the base state .Since 1867, Goppelsroder has carried out the fluorescence analysis for the first time in history, in addition to the general fluorescence analysis, such as the nonfluorescence substance being transferred into fluorescence ones which can be determined, fluorescence quenching method, energy transferring method and so on, with the introduction of laser, computers and advancement of electronics, synchromous fluorimetry , deivative fluorimetry , time-resolved fluorescence , fluorescence polaruzation, fluorescence immunoassay, low temperature fluorescence and other methods have greatly developed, accelerating the advent of all kinds of new fluorescence instruments.Now, the fluorescence analysis has being concerned inbiological reaction control, biosensor, pharmaceuticals minitor, biochemical analysis and multi-content in complicated system. And it has been developed into an important effective spectrum analysis technology. The thesis consists of six parts:Part one Determination of Ceftriaxone Sodium by Quenchingof Flow Injection ChemiluminescenceIntensity chemiluminescence can be emitted by oxidation reaction of potassium permanganate with acridine orange in sodium hydrogen medium, chemiluminescence is greatly quenched by the addition of Ceftriaxone Sodium .The calibration curve is linear over the range of 2~20mg/L.The detection limit is 8ng/mL. A 3.1% relative standard deviation is obtained in a repeatability study (n=11) at the 10mg/L level of ceftriaxone sodium. The method has been applied to determine ceftriaxone sodium in pharmaceutical with good result.Part two Study on Chemiluminesecence Reaction of Trimethoprim- KMnO4- Pyronine -H2SO4 by Flow Injection MethodA new CL reaction of Trimethoprim - KMn(V Pyronine -H2SO4 was studied by flow injection method.The factors of influencing CL intensity were investigated .A new method based on the CL reaction for the determination of TMP has been established by optimizing the experimental conditions.The linear range for the determination of TMP was 1.0 X10-4 mol/L~3.0 X 10-7mol/L and the determination limit was 3.4 X 10-9mol/L .The relative standard for the determination of 5.0 X 10-5mol/L TMP (n = 11) was 2.6% .The method has been used to determination in tablets and the results were in good agreement with those obtained by classical method .Part three Determination of Piroxicam by Quenchingof The Chemiluminescence A method is described for the determination of Piroxicam by quenching of thechemiluminescence signal of the acridine orange-KMnO4-OH-. The method is based on the fact that the concentration of potassium permanganate solution is lower by the reaction of permanganate with Piroxicam.The method is sensitive, the limit of detection is 4.5 X 10-6g/mL. The linear range of the working curves is 1.0X10-5~7.0X 10-4 g/mL. The relative standard devia...
|
PDF Full Text Request