| Owing to its distinguished advantages such as high sensitivity, wide linear range, rapid determination, simple instruments and easy automatization,Chemluminescence (CL) method has been extensively applied in various areas including environmental analysis, biochemical analysis, pharmic analysis and industrial analysis. However, it is difficult to detect CL because light emissions of some CL systems are too weak. So it is very necessary to develop enhanced CL systems. In this thesis, there kinds of chemiluminescence systems based on the enhancement effects of sodium hexametaphosphate (SP) or sodium hypochlorite were developed, and their analytical applications were also investigated combining flow-injection technique, the details are summarized as follows:1. A CL system CH3SHNH2-KMnO4, in which the emission intensity was greatly enhanced by the presence of sodium hexametaphosphate (SP), was developed. We found that the CL emission intensity of this CL system was remarkably inhibited when 6-mercaptopurine (6-MP) was also presented in the reaction system. Based on this, an inhibited CL method combining flow-injection technique for determination of 6-MP was established. Under the optimum experiments, the peak height was proportional to logarithmic 6-Mercaptopurine concentration in the range of 7.0×10-10 g/mL to 1.0×10-7 g/mL and the detection limit was 1.9×10-11 g/mL. The relative standard deviation was 1.5% for the determination of 8.0×10-8 g/mL 6-Mercaptopurine (n=11). The proposed sensor had been successfully applied to the analysis of 6-Mercaptopurine in human serum samples with satisfactory results.2. A CL system CdTe-KMnO4, in which the emission intensity was greatly enhanced by the presence of SP, was developed. We found that the CL emission intensity of this CL system was remarkably increased when L-ascorbic acid was also presented in the reaction system. Based on this, an enhanced CL method combining flow-injection technique for determination of L-ascorbic acid was established. Under the optimum experiments, the peak height was proportional to log L-ascorbic acid concentration in the range of 1.0×10-9 mol/L 5.0×10-6 mol/L and the detection limit was 2.9×10-10 mol/L. The relative standard deviation was 2.3 % for the determination of 5.0×10-7 mol/L L-ascorbic acid (n =11).3. A CL system CdTe-H2O2, in which the emission intensity was greatly enhanced by the presence of sodium hypochlorite, was developed. We found that the CL emission intensity of this CL system was remarkably inhibited when estrogens were also presented in the reaction system. Based on this, a flow-injection chemiluminescence method for the determination of estrogens including estradiol, estriol and estrone was proposed. Under the optimum experimental conditions, the linear ranges for estradiol, estriol and estrone were 1.0×10-6 1.0×10-4 mol/L, 3.0×10-7 7.0×10-5 mol/L and 1.0×10-7 1.0×10-4 mol/L respectively.
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