Study On The Binding Characteristics Of Aminopyrine And Paclobutrazol Imprinting Polymer

Molecular imprinting technique (MIT) is a technique to prepare polymers with recognition sites having a predetermined selectivity for given molecules. Polymerization is occurred between functional monomers and crosslinker around a template. After extration of the template from polymer, the molecularly imprinted polymer (MIP) has memory to the template. Much more attention has been paid to this technique in recent years. Molecularly imprinted polymers (MIPs) have been applied to chromatographic stationary phase, artificial antibodies, catalysts, sensors and drug assay tools. In normal condition, molecularly imprinted polymers only have effective recognition in non-polar or weak polar organic solutions. Strong polar solution or water could disrupt the template-monomer interaction. However, for some biological samples whose biological recognition mainly occurs in aqueous solution and the application of MIPs were limited to some extent. So it is extremely important to prepare MIPs that are able to go on biological recognition in aqueous solutions. In this paper, it was consisted of the following three parts. Chapter one: The development, principle and application of molecular imprinting technology were introduced in this paper. Chapter two: The molecularly imprinted polymer with aminopyrine as template was prepared by in-situ polymerization. The adsorption property and selective recognition ability of the imprinted polymer in pure water and additives-water media was studied respectively. The result showed that the polymer rod column had higher adsorption and higher selective ability in pure water mobile phase. The additives, such as HAc, N(CH2CH3) 3 and ion pair solvent SDS in the water phase, had very strong influence to the analytes'retention behavior with a dramatic decrease of the retention factors. Chapter three: In this experiment an effective synthetic procedure for preparation of molecularly imprinted polymers was used. The polymerization process was initiated by γ-radiation, in the model system with paclobutrazol as a template molecule. The experiment revealed that the polymer prepared with paclobutrazol as template showed higher and better selective recognition abilities to paclobutrazol. But the non-imprinted polymer had no such...