The Analysis Of Acetaniprid And Sulfamethoxayole With Molecular Imprinting And Relaed Technology

Molecular imprinting technique (MIT) is a polymerizing technique to produce molecular recognition materials with high selectivity and affinity. It has draw increasing attention because of its higher molecular recognition ability and stability .MIT has been used in a wide rang of applications .such as chiral separation .solid phase extraction .sensors,etc .In this thesis ,A molecularly imprinted pilymer highly selective for acetaniprid and cefaclor were prepared by molecular imprinting technique. Its selectivity to acetaniprid and cefaclor were evaluated by batch adsorption experiments .Molecularly imprinted polymers based on sulfamethoxazole has been used as HPLC stationary phases,The preparation with close analogues was simple .There are three chapters in this thesisIn chapter one :The principle of MIP polymerization methods, application of this technique and its future developments were introduced.and the applications of HPLC on separation and HPLC-ELSD technology are also described..In chapter two : A molecularly imprinted polymer highly selective for acetaniprid and cefaclor were prepared by molecularly imprinted technique . Its selectivity to acetaniprid and cefaclor were evaluated by batch adsorption experiments, which showed that acetaniprid-imprinted polymer and cefaclor -imprinted polymer exhibited higher affinity to acetaniprid and cefaclor.In chapter three :Sulfamethoxazole was employed as a template for the preparation of in-situ molecularly imprinted polymer rods for use as stationary phases of liquid chromatography,High performance liquid chromatography was employed to study the recognition property of the resulting Mff ,It was shown that the Sulfamethoxazole imprinted polymer has specific selectivity and high affinity to the sulfamethoxazole.The interaction between the MIP and target molecules were found to arise from hydrogen bonding in the imprinted area and the hydrophobic interaction in the non-imprinted area .The effects of water and flow rate on the separation, The preparation procedure was simple,The results showed that was more strongly retained than its analogues in the imprinted polymer rod-type column.