Studies On Isolation, Purification And Antitumor Activity Of Polysaccharides From Pleurotus Ferulae

Two polysaccharides(PFW, PFA)were isolated from fruit body of Pleurotus ferulae by using hot water, 3%trichloroacetic acid, respectively. Proteins were removed from PFW and PFA by Sevag method. After DEAE-Cellulose chromatography, PFland PF2were isolated from PFW, and PFA was further fractionated and purified, then PF3 was obtained.The weight-average molecular weight was determined by using multiangle laser photometer combined with GPC, the results showed:PFl contains two fractions, the weight-average molecular weights(Mw) are 7.875 105 and 3.245 104, the Mw of PF2 is 3.187 106,the Mw of PF3 is 1.975 106.The components and structure of polysaccharides were analyzed by IR and GC. The polysaccharide PF1 consists of rhamnose, glucose, galactose, mannose; PF2 and PF3 consist of rhamnose, xylose, glucose, galactose, mannose; PF1 contains -glucoside and mannoside, PF2 and PF3 contain a -glucoside.It was found polysaccharides have signficant antitumor activity on the implanted Sarcoma 180 in mice, there were not obvious difference among the polysaccharides.