Studies On Submerged Fermentation Technology And Application Of Polysacchride In Pleurotus Ferulae Lenzi

This paper mainly included four aspects: screening of liquid culture medium of Pleurotus ferulae Lanzi, optimization of fermentation conditions and extraction, analyses of physical and chemical properties of polysaccharide , the manufacture of the functional beverage,the results of which were as follows:1. By monofactorial and orthogonal test, the optimal composition of liquid culture medium for the mycelia growth and the producing of intracelluar polysaccharide were respectively determined. The best culture medium(%) contained glucose2, sucrose2,starch1.3,bran2, soybean2,KH2PO40.2,MgSO40.2,ZnSO40.2,NaCl0.2。2. The optimal flask (250ml) fermentation conditions were as follows: culture volume was 125mL, initial pH 5.5, culture temperature was 250℃, rotation speed was 150r/pm. And get the optimal jar (5L) fermentation conditions kinetics curve.3. The optimal intracelluar polysaccharide extracting conditions are 15 times water ,100℃, 3h ,extract of polysaccharides 3 times, It's molecular weight was estimated to be 313.5×104,59.0×104,21.1×104,3.4×104 by HPLC. Through UV spectrum, the structure of intracelluar polysaccharide is chiefly linked byβ-glycoside bonds and dominated by glucopyranose.4. The optimum manufacture condition and technical parameters.for two kinds of Pleurotus ferulae Lenzi functional beverage were obtained by this research.