The Research Of The Extraction, Purification, Structure Analysis Of Xinjiang Pleurotus Ferulae Polysaccharide

In this paper, Using pleurotus ferulae lenzi (PFL) from province of Xinjiang as material. Pleurotusferulae lenzi polysaccharide (PFLP) was studied systemically, including optimization of extractionparameters, isolation and purification, purity identification, determine molecular weight andmonosaccharide composition, and clarify its basic chemical structure. Main results are listed as follows:1. Optimization for extraction of PFLPThe key extraction technical parameters of PFLP by compared traditional hot water extraction methodwith ultrasonic assistant method were investigated. Depend on the extraction rate of PFLP product. Theoptimal technological process determined by the orthogonal tests on the basis of single factor experiment.The result showed that the optimal extracting conditions of traditional hot water were mass/volumeratio1︰30, extraction temperature90℃,extraction time2h. The extraction rate of PFLP product wasabout9.01%. The optimal extracting conditions of ultrasonic assistant technology were mass/volume ratio1︰40, extraction time70min, extracting power160W, extraction temperature60℃. The extraction rate ofPFLP product was about11.03%. Considering various factors, ultrasonic extraction is the optimalextraction.2. Isolation, purification and purity identification of PFLPCompared the effect of de-protein on PFLP that precipitated by ethanol between sevag method,enzyme combined with sevag method and TCA method, the results showed enzyme method combined withsevag has higher de-protein efficiency. And the polysaccharides lose rate is22.56%and the proteinremoval rate is81.2%.The PFLP that has been removed protein was separated firstly through DEAE-52cellulosechromatography column and further purified through chromatography column of sephadex G-100. Weobtained two fractions, named as PFLP l, PFLP2. And they was tested and verified as singlepolysaccharide through ultraviolet absorption spectrometry, polyacrylamide gel electrophoresis andsephadex G-100column chromatography.3. Basic chemical structure of PFLPThe molecular weight of PFLP was determined by sephadex G-100gel filtration. The result showedthat the molecular weight of PFLP1and PFLP2were about5500~6000Da,7900~8400Da.Monosaccharide composition and molar ratio of PFLP1and PFLP2was identified by Gaschromatography (GC). The result showed that they were rhamnose︰mannose︰glucose︰galactose＝1︰1.66︰19.6︰3.98, rhamnose︰xylose︰mannose︰glucose︰galactose＝1︰0.40︰0.66︰6.76︰4.27.Results of periodate oxidation and smith degradation suggested that glu in PFLP1and PFLP2werelinked to produce glu backbone chain mainly by1�'6glycosidic bond. Glu backbone chain were branchedby1�'2and1�'2,6glycosidic bond and some termination glycosyl by1�'glycosidic bondin PFLP1andPFLP2. Rha, man and gal in PFLP1and rha, xyl, man and gal in PFLP2were linked to glu backbone chainor side chain mainly by1�'3glycosidic bond. Spectra of FTIR implied that there were pyranose rings in PFLP1. Finally, through the analysis by theAFM proved that PFLP1molecule had highly branched structure, even between the sugar chains formedring structure.