| In this study, we prepared nano rhizoma chuanxiong by superfine technology. Pharmacological effect of Chinese tradition medicine are not only due to the special chemical components, but also closely connected with the physical state. Pharmacological effect might well change paroxysmal. It is critical of combining nanotechnology and Chinese traditional medicine to improve the utilization ratio and rapid releasing.Using the super fine mill---MICROS, a typical Chinese traditionalmedicine- rhizoma chuanxiong is ultrafine grinded. So much factors such as processing, properties, and the ultrafine effect are systematically studied. The main body of the MICROS comprises of a casing, revolving main shaft in the casing and several sub-shafts interlocked with the rotation of the main shaft. The ring moves radially as much as the clearance allows by being pressed toward the inside wall of the casing. At this moment, the ring rotates itself around the sub-shaft by the friction force of the wall surface. This means that as the ring revolves in the casing it also rotates. The particles are held between the revolving ring and wall surface are subjected to compressing force created by the centrifugal force of the ring and the friction created by the rotating ring. This process generates pul-verization and dispersion of the particles. The results show that besides structure diversity of grinding machine, the mass percent concentration C(%) , rotational speed R (r/min), time t (min) all have significant influence on the average grain diameter d (run). The optimum processing is : the mass percent concentration C is from 4% to 6% , rotational speed R is from 1200 to 1500 r/min, time t>30min is enough.The integral optical microscope photos of chuanxiong contain all identified microscopic tissues. Optical microscope photos of chuanxiong after ultrafine grinding demonstrates that cell wall is broken and most cells are broken into pieces except that a few of small sized starch grain / Grease chamber and calcium oxalate crystal are still can be seen. The reason is medicine subject tofierce forward extrusion and tangential shearing force during the course of grinding. Comparing with common pulverized powder, super fine mill can grid cells up to nearly nano-size, so cellular active components can contact dissolvent even can enter dissolvent completely. It can minimize the loss of active components.The dissolution quantities in vitro of ferulic acid that one of active components of rhizoma chuanxiong is increased by MICROS processing. The dissolution quantities in vitro of ferulic acid before and after ultrafine grinding at the same complex external condition is calculated by external reference method. The results show that the dissolution quantities of super fine powder after ultrafine grinding is by 36.4% higher than that of original powder. The extraction quantities of TMP (tetram ethylpyrazine) before and after ultrafine grinding at the same complex external condition are calculated by external reference method. The results show that the quality of TMP from chuanxiong after ultrafine grinding is by 32.2% higher than that TMP from original chuanxiong.In conclusions, ultrafine rhizoma chuanxiong was successfully prepared by MICROS in this paper. The influence of processing parameters on pharmic effect, the different properties between ultrafine rhizoma chuanxiong and originally fine powder were systematically studied by optical microscope, Zeta Potential Analyzer and high-performance liquid chromatography(HPLC). The results illustrated that the cellular tissue of rhizoma chuanxiong were broken into pieces and dispersed stably and homogeneously, in addition, the dissolution quantities in vitro of ferulic acid and the extraction quantities of TMP were accelerated by micros processing. Last, the work condition is under common temperature that can minimize the loss of active components.
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