Experimental Study On Formula Guizhi Gancal Decoction By Semi-Bionic Extraction

Objective: Optimizing the extract technology in the modification of the formula of Guizhi Gancao Decoction and further verifying the correctness and practicality of semi-bionic extraction(brief name SBE method). Methods: At first, optimizing the SBE technical conditions for Guizhi Gancao Decoction ingredients through even design while Glycyrrhetinic Acid, Cinnamic Acid, total flavones, volatile oil and dry extract were adopted as indexes; Then, dividing the ingredients into 2 combinations and extracting by the SBE method respectively, determining the contents of index components in the each extract, comparing the total integral calculus area of HPLC by gradient washing, then make out the SBE s best ingredients combination For the formula of Guizhi Gancao Decoction. The SBE extract of the optimized ingredients combination was put to further comparison of components in different conditions of alcohol precipitating concentrations, confirming the most ideal alcohol precipitating concentration of the semi-bionic extraction by precipitation with alcohol (SBAE) for the formula. On this base, comparison of components were conducted over the four extracts derived from four different kinds of extraction methods wich are the SBE method, the water extraction (WE) method, SBAE method, and the water extraction by precipitation with alcohol(WAE) method. Results: The technical condition of SBE for the formula of Guizhi Gancao Decoction was: The pH value of each section were2.00 7. 20> 8.50, the durations of each section were 190min. 90min and 50min respectively. It is better to extract Radix Glyeyrrhizae and Cortex Cinnamomi together. The comprehensive values assessed by the contents of 5 index components in the four extracts were: SBE>WE>SBAE>WAE. Conclusion: SBEmethod is the best among the four methods in the extraction of Guizhi Gancao Decoction components. The study firmly verifies that the SBE method is correct and practica, consummates the basic study model of Traditional Medicine compound' s modification by using the SBE method, provides some experiences for Traditional Medicine s matching study.